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A New Cancer Cell Detection Method Using an Infectivity-enhanced Adenoviral Vector
Uchino, Junji,Takayama, Koichi,Nakagaki, Noriaki,Shuo, Wang,Hisasue, Junko,Nakatom, Keita,Ohta, Keiichi,Hirano, Ryosuke,Tashiro, Naoki,Miiru, Izumi,Fujita, Masaki,Watanabe, Kentaro,Nakanishi, Yoichi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Cytological examination is widely used as a diagnostic tool because of the ease of collecting cells from the involved area. However, the diagnostic yield of cytological examination is unsatisfactory; the reasons include sampling error, poorly prepared samples, small numbers of malignant cells, and low grades of cellular atypia. In this study, we focused on the high infectivity of adenovirus towards epithelial cells and applied the luciferase-expressing adenoviral vector to a new cancer cell detection tool. In addition, adenoviral infectivity was enhanced by modifying viral fiber proteins. The sensitivity of the diagnostic tool was tested using the NCI-H1299 lung cancer cell line, and validated in body fluid samples from cancer patients with a variety of etiology. Results showed that the adenovirus efficiently transfected NCI-H1299 with high sensitivity. Only 10 cancer cells were sufficient for detection of luciferase signals. In body fluid samples, the adenovirus confirmed the diagnosis for malignant and benign cancer, but not in non-epithelial cell derived samples. This study provides proof-of-concept for a more reliable and sensitive diagnostic tool for epithelium-derived cancer.
( Mariko Mochizuki ),( Yukari Osada ),( Nami Shimaoka ),( Mayumi Ito ),( Naomi Sannda ),( Junko Fujita ),( Hiroyuki Matumoto ),( Tadashi Matushita ) 대한임상병리사협회 2015 임상미생물검사학회 발표자료집 Vol.2015 No.-
Case The patient was a 45-year-old man. He was diagnosed as having malignant melanoma ten years ago, and received chemotherapy including alkylating agents. After nine years, he developed treatment-related myelodysplastic syndrome / myeloproliferative neoplasm (MDS / MPN), and he received hematopoietic stem cell transplantation. He had recurrence as MDS and re-transplanted. The blood culture after re-transplantation were repeatedly positive for Enterococci and yeast-like fungi of black-colored colony. Micafungin (MCFG) and Fluconazole were added to the treatment with liposomal-amphoterisin B. Hematopoetic stem cell transplantation was performed again due to bone marrow graft failure, however, his condition got worsened and died. Microbiological findings A yeast-like form with budding was found under microscopy, and black-colored colonies were grown on a CHROMagarTM Candida plate (Becton Dickinson). Species identification was not possible using VITEK and ApiC AUX (bioMerieux). Further culture revealed brown filamentous form on the melted chocolate-like colonies, which was strongly suggestive of dimorphic fungi. This fungus was identified as Exophiala dermatitidis by sequence analysis of ITS region of rRNA gene. Susceptibility test using the ASTY (KYOKUTO PHARMACEUTICAL INDUSTRIAL Co., Ltd) showed that, after 72hr growth, a high MIC values of > =16㎍/ml in MCFG and relatively good susceptibility to other antifungal drugs. Discussion Exophiala dermatitidis is a type of pathogenic dematiaceous fungi which was widely found in residential environment. It mainly causes infections of cutaneous and subcutaneous tissue in immunocompromised patients, but sometimes fatal infections such as bloodstream infection. In clinical settings, it is important to speculate this species from morphological features, which will lead to the early diagnosis and treatment especially in immunocompromised cases. Acknowledge Takashi Yaguchi, Aki Hirabayashi, Daizo Kato, Mitsutaka Iguchi, Satoshi Morioka, Tetsuya Yagi
Essential protocols for in vitro evaluation of nanoparticle
Hitoshi Iwahashi,Masanori Horie,Keiko Nishio,Shigehisa Endoh,Haruhisa Kato,Katsuhide Fujita,Shinichi Kinugasa,Arisa Miyauchi,Ayako Nakamura,Junko Takahashi,Etsuo Niki,Yasukazu Yoshida,Junko Nakanishi 한국환경독성학회 2010 한국독성학회 심포지움 및 학술발표회 Vol.2010 No.11