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Jung-Pil Suh,Ji-Ung Jeung,Jong-Hee Lee,Jeom-Ho Lee,Sae-Jun Yang,Yeon-Gyu Kin,Kshirod K. Jena 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Brown planthopper (BPH) is a major insect pest of tropical indica and temperate japonica rice in Asia and Africa. A major BPH resistance gene, Bph18 derived from IR65482-7-216-1-2 has been fine mapped on chromosome 12 and confers strong resistance to the Korean biotype of BPH. The Bph18 gene is tightly linked to the STS marker, 7312.T4A and is non-allelic to previously reported resistance genes present on chromosome 12. The Bph18 gene has been transferred into two elite japonica cultivars (Jinbubyeo and Junambyeo) background through marker-assisted backcross breeding (MAB) strategy. Foreground selection using STS markrs linked to the Bph18 gene in advanced backcross progenies confirmed homozygous marker alleles associated with BPH resistance. Background selection of the breeding lines with 260 simple repeat (SSR) markers revealed rapid conversion toward recurrent parent genotypes with less donor chromosomal segments (5.3-16.7%). Major agronomic traits of the progenies were analyzed and some breeding lines have agronomic traits comparable to the recurrent parent. One breeding line (S.523) with multiple-resistance to BPH and major diseases, desirable agronomic traits and grain quality has been recommended for regional testing in Korea. MAB is the suitable strategy to incorporate new genes into susceptible japonica to develop elite breeding lines.
Jung Pil Suh,Young Chan Cho,Soo Jin Kwon,Im Soo Choi,Ha Chul Hong,Yeon Gyu Kim,Sang Nag Ahn,Hung Goo Hwang 한국육종학회 2006 한국육종학회지 Vol.38 No.2
A recombinant inbred lines (RILs) consisting of 231 lines, derived from a japonica (Suweon365) and a japonica (Chu-cheongbyeo) rice, was used to investigate the genetic factors affecting cooking and eating quality of rice. Alkali digestion valueloci (QTLs
Effectiveness of marker-assisted backcrossing breeding for biotic resistance in rice
Jung-Pil Suh,Kshirod K. Jena,Young-Chan Cho,Ji-Ung Jeung,Yong-Jae Won,Im-Soo Choi,Jeom-Ho Lee,Myeong-Ki Kim,Chung-Kon Kim 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
The transfer of a biotic resistance gene from indica rice cultivars into japonica cultivars by conventional breeding methods often difficult due to high sterility of the progenies, poor plant type, and linkage drag. Molecular markers provide opportunities to map resistance genes and accelerate the application of marker-assisted backcross(MAB) breeding through the precise transfer of target genomic regions into the recurrent parent. The basis of MAB breeding is to transfer a specific gene/allele of the donor parent into the recurrent parent genome while selecting against donor introgressions across the rest of the genome. The effectiveness of MAB breeding depends on the availability of closely linked DNA markers for the target locus, the size of the population, the number of backcrosses and the position and number of markers for background selection. We have successfully developed Bph18 version of the commercially cultivated japonica elite cultivar by using MAB and incorporating the resistance gene Bph18 that conferred enhanced resistance to BPH. MAB breeding provides a new opportunity for the selective transfer of biotic resistance genes into elite indica rice cultivars devoid of linkage drag. In additon, molecular markers precisely estimate the introgression of chromosome segments from donor parents and can speed up the recipient genome recovery via background selection.
Identification of QTLs for Cold Tolerance at Seedling Stage in Korean Weedy Rice (Oryza sativa L.)
Jung Pil Suh,Sang Nag Ahn,Im Soo Choi,Young Chan Cho,Hung Goo Hwang,Hak Soo Suh 한국육종학회 2003 한국육종학회지 Vol.35 No.2
Molecular markers were used to map and characterize quantitative trait loci (QTLs) for traits related to cold tolerance in an intrasubspecif ic backcross population of r ice. The parents of the cross were a cold susceptible Tongil-type cultivar 'Milyang23
Field Performance and SSR Analysis of Drought QTL Introgression Lines of Rice
( Jung Pil Suh ),( Yong Jae Won ),( Eok Keun Ahn ),( Jeong Heui Lee ),( Woon Goo Ha ),( Myeong Ki Kim ),( Young Chan Cho ),( Eung Gi Jeong ),( Bo Kyeong Kim ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.2
A set of five Korean rice cultivars and seven drought-tolerant indica lines were screened under irrigated non-stress and drought-stressed conditions in the 2011 and 2012 dry seasons at IRRI, Philippines. The drought-stressed experiment received mild to moderate stress. Under drought stress, ‘IR86918-B-439-B’ had the highest grain yield among all tested lines and ‘Hanarembyeo’ had the highest grain yield among the five Korean rice cultivars. ‘IR86918-B-439-B’ also had the highest yield under irrigated non-stress conditions. The grain yield of ‘Hanareumbyeo’ was similar to ‘IR86918-B-439-B’ under non-stress conditions. SSR marker analysis was performed using 125 SSR markers for detection of polymorphic markers between the Korean rice cultivars and the drought-tolerant indica lines, and for genetic diversity analysis. Twelve polymorphic markers were identified in the region of three major drought QTLs (DTY1.1, DTY2.2, DTY3.1) in two of the Korean rice cultivars and three of the drought-tolerant lines. These polymorphic markers will be useful as foreground genotyping markers for drought-QTL introgression in Korean rice genetic backgrounds.
Jung-Pil Suh,Arvind Kumar,Mallikarjuna Swarmy,Eok-Keun Ahn,Young-Hwan Yoon,Young-Chan Cho,Myeong-Ki Kim,Chung-Kon Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
A set of nine Korean rice germplasm (KRG) along with the six indica lines were screened under irrigated non-stress and drought stress situations at IRRI in dry season (DS) 2011. The experiment received mild to moderate drought stress. Under irrigated situation, among all lines, IRRI119 yielded highest followed by PSBRc80 and PSBRc14. Among nine KRG, Hanareumbyeo yielded highest followed by Gayabyeo. Yield of Hanareumbyeo was similar to high yielding indica lines. Under drought, PSBRc14 provided the highest yield among indica lines and Hanarembyeo provided highest yield among nine KRG. Among nine KRG, Hanarembyeo provided the highest yield both under irrigated non-stress and drought stress situation. Parental polymorphism was performed with 125 SSR markers taking six KRG and three drought tolerant donors and polymorphic markers and japonica lines background specific markers were identified. The polymorphic markers in the region of three QTLs (DTY1.1, DTY2.2, DTY3.1) will be used for foreground genotyping for QTL introgression and background specific markers will be used for background genotyping. Sixteen rice germplasm could be separated into two main groups, japonica and indica groups by cluster analysis. The japonica and indica groups also classified as two subgroups, respectively. Based on results of screening of japonica lines under irrigated non-stress and drought stress situations, two KRG- Hanarembyeo and Jinmibyeo were selected for introgression of three QTLs (DTY1.1, DTY2.2 and DTY3.1) associated with grain yield under drought stress.
Jung-Pil Suh,Jae-Hwan Roh,Young-Chan Cho,Seong-Sook Han,Yong-Hee Jeon,Kyung-Ho Kang,Yeon-Gyu Kim 한국육종학회 2008 한국육종학회지 Vol.40 No.3
Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.
Jung-Pil Suh,Sang-Nag Ahn,Young-Chan Cho,Kyong-Ho Kang,Im-Soo Choi,Yeon-Gyu Kim,Hak-Soo Suh,Hung-Goo Hwang 한국육종학회 2005 한국육종학회지 Vol.37 No.5
An interspecific BC3F2 population (Milyang 23/O. glaberrima/3*Milyang 23) consisting of 141 lines was evaluated for8 traits associated with yield. Oryza glaberrima (IRGC #103544) was phenotypically inferior to all of the traits examined in thisicle and grain yield. A genetic linkage map generated for this population consisted of 124 SSR markers with an average intervallength of 13.1 cM. The percentage of O. glaberrima genome, which was calculated on the number of the exotic gene introgres-sions contained in the 141 BC3F2 lines, ranged from 3.9 to 25.4% with a mean of 12.8%. Of the 37 QTLs associated with yield andyield components, 24 alleles from O. glaberrima were favorable. However, eight QTLs were significantly associated with yieldand five alleles of O. glaberrimafrom 1.5 to 2.2 tons/ha. Exploitation of the genetic potential of exotic species using molecular markers might provide a new oppor-tunity to improve yield and quantitative traits in rice breeding program.
Suh, Jung-Pil,Jeung, Ji-Ung,Noh, Tae-Hwan,Cho, Young-Chan,Park, So-Hyun,Park, Hyun-Su,Shin, Mun-Sik,Kim, Chung-Kon,Jena, Kshirod K Springer New York 2013 Rice Vol.6 No.-
<P><B>Background</B></P><P>The development of resistant cultivars has been the most effective and economical strategy to control bacterial leaf blight (BB) disease of rice caused by <I>Xanthomonas oryzae</I> pv. <I>oryzae</I> (<I>Xoo</I>). Molecular markers have made it possible to identify and pyramid valuable genes of agronomic importance in resistance rice breeding. In this study, three resistance genes (<I>Xa4</I> + <I>xa5</I> + <I>Xa21</I>) were transferred from an indica donor (IRBB57), using a marker-assisted backcrossing (MAB) breeding strategy, into a BB-susceptible elite japonica rice cultivar, Mangeumbyeo, which is high yielding with good grain quality.</P><P><B>Results</B></P><P>Our analysis led to the development of three elite advanced backcross breeding lines (ABL) with three resistance genes by foreground and phenotypic selection in a japonica genetic background without linkage drag. The background genome recovery of the ABL expressed more than 92.1% using genome-wide SSR marker analysis. The pathogenicity assays of three resistance-gene-derived ABL were conducted under glasshouse conditions with the 18 isolates of <I>Xoo</I> prevalent in Korea. The ABL exhibited very small lesion lengths, indicating a hypersensitive reaction to all 18 isolates of <I>Xoo</I>, with agronomic and grain quality traits similar to those of the recurrent parent. Pyramiding the resistance genes <I>Xa4, xa5</I> and <I>Xa21</I> provided a higher resistance to <I>Xoo</I> than the introduction of the individual resistance genes. Additionally, the combination of two dominant and one recessive BB resistance gene did not express any negative effect on agronomic traits in the ABL.</P><P><B>Conclusions</B></P><P>The strategy of simultaneous foreground and phenotypic selection to introduce multiple R genes is very useful to reduce the cost and the time required for the isolation of desirable recombinants with target resistance genes in rice. The resistance-gene-derived ABL have practical breeding value without a yield penalty by providing broad-spectrum resistance against most of the existing isolates of BB in South Korea and will have a high impact on the yield stability and sustainability of rice productivity.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/1939-8433-6-5) contains supplementary material, which is available to authorized users.</P>