흰쥐 간장에 존재하는 카드뮴 결합 단백질의 정제 및 아미노산 조성에 관한 연구

한중수 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.2

In order to investigate whether the newly synthesized cadimium(Cd)-bound metallothionein(MT) accumulates in livers of rats which have received subcutaneous injections of Cd, isolation and purification of these protein were performed using Sephacryl S-200 and DEAE-Sephadex column chromatographies, and SDS-PAGE. And PICO.TAG HPLC analysis of amino acids was carried out to find out amino acid composition of MT-II. To confirm that the newly synthesized components are protein in nature, the Cd-bound fractions were examined for incorportation of [??S]cysteine. The effect of actinomycin-D on the synthesis of MT was also studied. 1. Cd induced newly synthesized peak which was identified by gel filtration on a Sephacryl S-200 column. This peak was detectabel only by measurement of Cd and zinc by atomic absorption analysis, and was labelled with [??S]cysteine. These results indicated that the peak components were protein which bound with Cd and zinc, e.g. MT. 2. MT was resolved into two isoforms i.e. MT-I and MT-II that had an apparent molecule mass of 10,000 by ion-exchange chromatography on a DEAE-Sephadex A-25 column 3. Formation of MT was completely abolished by actinomycin-D treatment. 4. SDS-polyacrylamide gel electrophoresis of MT-II showed the presence of a single band which appeared as a single peak on scanning at 590nm. 5. The most typical characteristic of MT-II was a extremely high cysteine content(32.8%) followed by lysine, serine and alanine. Also MT-II showed completely lacking in aromatic amino acids and histidine. These results suggested that newly synthesized MT-II containing high cysteine content was theh major isometallothionein and might have protective role against the toxic effects of Cd through the formation of metal-thiolate complexes and that the induction of formation of MT was due to the de novo synthesis of the protein after the induction ofmRNA synthesis by Cd.

급성 백혈병환아의 혈청내 변형 Ribonucleoside의 변동에 관한 연구

한중수,이정국,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

In order to find out whether the modified ribonucleosides in serum could be used as specific tumor markers for acute lymphocytic leukemia (ALL) and acute myelogenous leukemia (AML), analysis of serum samples from normal child hood controls and patients with the leukemia was performed using reversedphase HPLC. For this study, a rapid and precise chromatographic method for the determination of very small amounts of modified nucleosides in serum by HPLC has been developed. The ribonucleosides were prefractionated with boronate affinity gel column. 1. The concentration of total modified nucleosides in serum of patients with ALL was increased by 46% as compared to normal control level. Patients with ALL had significantly higher concentrations of pseudouridine (ø), 1-me-thyladenosine(m A), 4-thiouridine(sU), 1-methylinosine(mI), 1-methylguanosine(m G ), N,N-dimethylguanosine(m G ) and -methyl-adenosine( ) than normals. Among these nucleosides elevated, positive rates of ø, m A, m I, m G and N A as a marker for the leukemia were observed to be 79%,79%,64%,93% and 93%, respectively. 2. When compared to normal control value, total modified nucleoside content in serum of patients with AML was increased by 21%, Contents of , mA, sU, mI,mG and NA were significantly incresed in serum of patients with AML. Positive rate of mI, mG and NA were 83%, and that of mA was 67%, respectively in AML studied in this experiment. 3. In follow-up study for seven of fourteen patients with ALL, contents of , mA, mI and mG were decreased to normal value after effective chemotherapy. The results suggested that serum levels of ø, m A,m I, m G and N A for ALL and those of m A, m I, m G and N A for AML could be used as diagnostic or biochemical markers. These markers might be useful to monitor the effectiveness of chemotherapy for leukemia.

좌우각도 변화에 따른 안면 특징점 변화

한한수,김석경,이중,남금문 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

분산중합을 이용한 Poly(MMA-co-2-HEMA)의 고 고분자량의 합성 및 특성 연구

임수빈,현수용,한병학,김형중 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

Poly[methyl methacrylate(MMA)-co-2-hydroxyethyl methacrylate(2-HEMA)] particles were synthesized by dispersion polymerization in a cyclic hydrocarbon medium. The reactions were carried out at 72~80℃ in the presence of SIS block copolymer and t-butyl peroxy-2-ethyl hexanoate, as steric stabilizer and initiator, respectively. The used cyclic hydrocarbon medium were cyclohexane and methyl cyclohexane. The resulting poly(MMA/2-HEMA) latexes were found to be monodisperse particle diameter of 2~3㎛. Dispersion polymerization of poly(MMA/2-HEMA) were carried out to investigate the effect of concentration of initiator. The molecular weight of poly(MMA/2-HEMA) in presence of methyl cyclohexane than cyclohexane was increased.

경골 골수강내 금속정 삽입술시 적절한 도입부에 대한 해부학적 분석

강수용,이은우,강기서,이한준,정호중,정평호 대한골절학회 2004 대한골절학회지 Vol.17 No.2

Gunn Diode Dipole Layer Mode의 周波數 特性

金秀重,朴成漢 경북대학교 공과대학 1974 工大硏究誌 Vol.3 No.-

When the n_0L product is greater than 10^12 cm^-2 in n-GaAs, the space charge perturbation in the material increases exponentially in space and time to form mature dipole layer which propagate to the anode. This is due to the inherent differential negative resistance characteristics of bulk n-GaAS. A velocity-field equation, whose characteristic is similar to that given by Ruch and Kino, was derived from the McCumber and Cynoweth's temperature model of electron distribution. Using this equation, a computer analysis was made to find out the variation of frequency of the Dipole Layer Mode of Gunn Oscillator due to the variation of notch width and doping donsity in n-GaAs bar. The result showed that the frequency is more affected by width than by doping density variation of the notch.

유전율이 다른 두 기판의 접합에 의한 마이크로스트립 불연속의 반사특성 해석

전중창,김태수,한수용 위덕대학교 산업기술연구소 2001 산업기술연구소 논문집 Vol.5 No.1

The finite-difference time-domain (FDTD) method has been applied to the analysis of microstrip discontinuities comprised of junctions of two substrates having different dielectric constants. The discontinuities treated in this paper include microstrip step-in-width on a single substrate for the validation of analysis method, and microstrip junctions of two different substrates where strip widths are varied to analyze the reflection characteristics numerically. It is noted that the result for the junction having a uniform strip width but different dielectric constants shows a similar characteristic with a conventional step-in-width discontinuity. The importance of this result is the implication that the junctions treated in this paper can be applied to the design of microwave passive circuits such as filters and matching networks.

소출력 송수신기용 광대역 안테나 개발

전중창,한수용 진주산업대학교 산업과학기술연구소 2007 산업과학기술연구소보 Vol.- No.14

As the wireless services are popular for convenience, antennas having wider bandwidth are required more often. In this paper, a new type of monopole antenna is invented for multiple band applications. Multiple band property for the proposed antenna is achieved by adjusting the coupling structure of the antenna base. -10 dB impedance bandwidths are 1.74-3.06 GHz and 5.59-10.62 GHz, which can cover various kinds of wireless services, such as PCS(1.75-1.87 GHz), IMT-2000(1.92-2.17 GHz), WiBro(2.3-2.39 GHz), WLAN (2.412-2.483 GHz, 5.725-5.825 GHz), DMB(2.63-2.655 GHz), High-band UWB(7.2-10.2 GHz).

영상분석 시스템 구현(Ⅰ)

김동욱,한한수,김석경,이중,하영호,김희수 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

토끼 간조직의 Stimulatory Guanine Nucleotide 결합 단백의 αsubunit 에 대한 cDNA 염기서열에 관한 연구

이상훈,노연희,한중수,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

G proteins, a family of guanine nucleotide-binding protein superfamily, are involved in transmembrane signaling systems as transducers. In this study cDNAs encoding αsubunit of the stimulatory G protein (G α ) from rabbit liver were cloned and their nucleotide sequences were determined : a λgt 11 cDNA library from total cellular poly (A)' mRNA in rabbit liber was screened for recombinant λDNA that codes for G α with labeled probe. The probe used was an EcoRI digested 3' end fragment of cDNA for G α from olfactory neuroepithelium of rat. Six of the approximately recombinant clones were screened by in situ hybridization and by autoradiography, detecting sixteen positive clones. These clones were tested using polymerase chain reaction (PCR) technique and thirteen of them were turned out to have two sets of conserved sequence in αcDNA. Four clonse among them were selected form analysis of their cDNA sequences, showing the presence of two types of cDNA, namely and with the total length of nucleotide sequence of1.392bp and 1.613bp, respectively . From nucleotide sequence analysis the amino acid residues of -1 and α-2 were deduced : they contain 335 and 386 amino acid residues (including the initiator methionine), respectively. The calculated molecular weights for and were 38.9kd and 44.6kd, respectively. The significant difference in size between two cDNAs for G αseemed to be due to the assumption that alternative promoter and leading exon might be involved in transcribing the mRNA for . Two types of cDNA from rabbit liver shared over 95% of amino acid homology with that from rat olfactory neuroepichelium if deleted portions are not counted. More information on the cDNAs could be obtained through further studies such as Sl nuclease protection experiment, expression and mutation study.