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Tran, The-Vinh,Shin, Eun-Joo,Dang, Duy-Khanh,Ko, Sung Kwon,Jeong, Ji Hoon,Nah, Seung-Yeol,Jang, Choon-Gon,Lee, Yu Jeung,Toriumi, Kazuya,Nabeshima, Toshitaka,Kim, Hyoung-Chun Elsevier 2017 Food and chemical toxicology Vol.110 No.-
<P><B>Abstract</B></P> <P>We investigated whether ginsenoside Re (Re) modulates phencyclidine (PCP)-induced sociability deficits and recognition memory impairments to extend our recent finding. We examined the role of GPx-1 gene in the pharmacological activity of Re against mitochondrial dysfunction induced by PCP in the dorsolateral cortex of mice. Since mitochondrial oxidative stress activates NADPH oxidase (PHOX), we applied PHOX inhibitor apocynin for evaluating interactive modulation between GPx-1 and PHOX against PCP neurotoxicity. Sociability deficits and recognition memory impairments induced by PCP were more pronounced in GPx-1 knockout (KO) than in wild type (WT) mice. PCP-induced mitochondrial oxidative stress, mitochondrial dysfunction, and membrane translocation of p47<I>phox</I> were more evident in GPx-1 KO than in WT. Re treatment significantly attenuated PCP-induced neurotoxic changes. Re also significantly attenuated PCP-induced sociability deficits and recognition memory impairments. The attenuation by Re was comparable to that by apocynin. The attenuation was more obvious in GPx-1 KO than in WT. Importantly, apocynin did not show any additional positive effects on the neuroprotective activity of Re, indicating that PHOX is a molecular target for therapeutic activity of Re. Our results suggest that Re requires interactive modulation between GPx activity and PHOX (p47<I>phox</I>) to exhibit neuroprotective potentials against PCP insult.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genetic depletion of GPx-1 facilitates PCP-induced mitochondrial dysfunction, oxidative parameters and PHOX activity. </LI> <LI> Genetic depletion of GPx-1 facilitates sociability deficits and recognition memory impairments induced by PCP. </LI> <LI> Re-mediated protective potentials against PCP required the interactive modulation between GPx-1 and PHOX (p47<I>phox</I>). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Hyoung-joo Ham,Geun-ho Kang,Yun-sang Choi,Tae-jun Jeong,Ko-eun Hwang,Cheon-jei Kim 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.5
The objective of this study was to evaluate effects of Gaeddongssuk powder (GP) on quality characteristics and shelf stability of emulsion sausages during storage. Proximate composition properties showed no significant differences in all treatment (p>0.05). Control showed the highest cooking loss while the treatment with GP showed decreased cooking loss depending on increasing GP content (p< 0.05). Apparent viscosity of batter was increased as the amount of GP increased, whereas hardness of emulsion sausages was decreased with increasing GP level. In sensory evaluation, emulsion sausage with 0.1% GP resulted in the highest score in overall acceptability. The pH values of all treatments decreased at the early storage stage, followed by gradual increase. The lightness and redness of treatments were decreased when the level of GP was increased. However, the yellowness of sausages with GP were higher than that of control (p0.05). Therefore, Gaeddongssuk powder up to 0.1% has a potential as a natural antioxidant for meat products because it can inhibit lipid oxidation of sausages without decreasing their sensory properties.
Hyoung-Joo Kim,Joohyun Shim,Nayoung Ko,Yongjin Lee,Jae-Kyung Park,Kyungmin Kwak,Jun-Hyeong Kim,Pulip Kang,Jeong-Woong Lee,Hyunil Kim,Kimyung Choi 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
The COVID-19 pandemic is caused by SARS-CoV-2, which continues to raise, public health concerns worldwide. Coronaviruses have an outer crown-like spike protein that binds to the human Angiotensin-converting enzyme 2 (ACE2) gene, resulting in infection via endocytosis. Therefore, research on hACE2 is expected to be critical for developing our understaining of COVID-19. To facilitate this, we developed Yucatan miniature pigs expressing hACE2 as animal models for COVID-19 research. First, vector containing hACE2 gene, FLAG tag, and GFP was constructed using the CMV promoter. Three lines of stable cell lines with hACE2 protein expression were created by transfecting Yucatan miniature pig ear fibroblasts with the constructed vector. The established cells then underwent somatic cell nuclear transfer, and were transferred to surrogate sows as donor cells, resulting in the successful production of four transgenic cloned pigs. PCR analysis confirmed that the hACE2 gene was inserted into the genome, and that hACE2 mRNA was well-expressed in the lung, heart, and small intestine. In addition, differences in protein expression between transgenic clones and wild-type pigs were confirmed using ear fibroblasts. Finally, karyotyping and fluorescence in situ hybridization analysis revealed that the transgenic cloned pigs had the same number of chromosomes as normal pigs (36 chromosomes, plus two sex chromosomes), and that the hACE2 gene was inserted into chromosome 17. In this study, we succesfully produced transgenic Yucatan miniature pigs expressing the hACE2 gene for use as prelinical COVID-19 target models. *This work has supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (NRF-2020M3A9I2105803).
Generation of the GGTA1/CMAH/hCD46 Genetically Modified Pigs for Xenotransplantation
Hyoung-Joo Kim,Joohyun Shim,Nayoung Ko,Yongjin Lee,Jae-Kyung Park,Kimyung Choi 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
The demand for organ transplantation has rapidly increased all over the world during the past decade. Genetically modified pigs provide a solution to the severe shortage of organs available for human transplantation. Porcine α-1,3-galactosyltransferase (GGTA1) gene is generates Gal-T epitopes that trigger hyperacute rejection in pig-to-human transplantation. Since production of GGTA1 knock-out pigs in 2002, non-gal antigens are considered to be the next xenoantigen involved in the rejection phenomenon. Here, we targeted the GGTA1 and CMP-Neu5Ac hydroxylase (CMAH) genes with CRISPR-Cas9 systems resulting in double knock-out pigs that no longer express α-Gal or Neu5Gc. Similar to GGTA1 gene, CMAH is widely expressed on the endothelial cells of many mammals except humans and this epitope is a potential porcine target for the antinon- gal antibody in humans. CMAH is responsible for the expression of Neu5Gc that key non-gal antigen. Additionally, hCD46 controls complement activation and when this gene expressed sufficiently as a transgene protects xenografts against complement-mediated rejection. This is report to describe generation of transgenic pigs that modify GGTA1, CMAH and hCD46. We expect to remove α-gal and Neu5Gc antigens and express hCD46 from pig for reducing human antibody mediated cytotoxicity.