초파리 자연집단에 대한 미토콘드리아 DNA와 수용성 단백질의 유전적 변이

추종길,조 현 中央大學校 遺傳工學硏究所 1997 遺傳工學硏究論集 Vol.10 No.1

D. melanogaster와 이 종의 근연종인 D. simulans를 재료로 종내 및 종간 분화과정을 규명하기 위하여 mitochondrial DNA (mtDNA)의 변이를 조사하였고, mtDNA분석 결과와 핵분석 결과를 비교하여 종의 유연관계를 추정하고자 TDE에 의한 수용성 단백질 분석을 실시하였다. D. melanogaster와 D.simulans의 mtDNA의 전체 genome size는 약 19.0kp로 추정되었다. D. melanogaster에서는 site variation (Hae Ⅲ 및 Sca Ⅰ)과 length variation (Hind Ⅲ)이 나타났다. 또한 4종류 (M1-M4)의 mtDNA genotype이 검출되었었으며, 종내 평균 염기치환율이 0.0033로써 낮은 지역분화를 나타내었다. 한편 D. simulans에서는 site variation 및 length variation이 검출되지 않았다. mtDNA와 핵내 DNA에서 유래된 TDE에 의한 cluster 분석 결과는 서로 일치하지 않았다. Mitochondrial DNA (mt DNA) polymorphism of two closely related species of Genus Drosophila (D. melanogaster and D. simulans) was investigated. mt DNA was isolated from 35 and 8 isofemale strains in D.melanogaster and D. simulans, respectively, and were analysed by eight restriction endonucleases. On the basis of mt DNA fragment differences, site variation appeared two restriction endonucleases, HaeⅢ and Sca I, and four genotypes were identified in D.melanogaster. Nucleotide substitution rates among four genotypes were ranging from 0.0032 to 0.0088 (average 0.0033). In addition to, 400bp length variation was found in eight geographical populations. A possible explanation of for low divergence was that mtDNA variation of eight geographical populations in D. melanogaster could not be accumulated sufficiently owing to recent divergence of few individuals, and that sequence divergence was prevented by frequent migration.

향류분배 장치에 의한 주광성 행동의 인위도태와 자연도태의 유전적 효과

추종길,한영주,임귀순 中央大學校 自然科學硏究所 1987 自然科學硏究所 論文集 Vol.1 No.-

Directional selections for positive and negative phototaxis were carried out with hte populations, derived from Jeju and L-F strain of Drosophila melanogaster, using the countercurrent distribution apparatus. Effects of selection for positive and negative phototaxis were clearly recognized in the early generations, and the populations were reached a selection plateau from the 5th generation onwards. After 9 generations of artificial selection, the populations selected for positive and negative phototaxis were relaxed in the following 16 generations. The phototactic responses to natural selection of positive and negative populations were not returned to their neutral states. As a result of artificial and natural selection, genes of phototactic behaviors measured by means of countercurrent distribution apparatus are possibly controlled by major genes, and they seem to be fixed in the early generations of artificial selection. Walking behavior in populations of flies with positive and negative phototactic respones at the 9th, 16th and 17th generations was measured by the connected test tube apparatus. The negative populations turned out slow walking behavior. It seems reasonable to assume that genes controlling negative phototaxis and slow walking behavior were accumulated and selected within populations simultaneously.

광주기변화가 초파이의 우화리듬에 미치는 영향

송기덕,추종길,한영주 高神大學校 1987 論文集 Vol.15 No.-

An examination was carried out with Drosophila melanogaster to analyze the genetic effect of different photoperiod on the mean eclsion time and eclosion rhythm. 1. The mean eclosion time in accordance with changes of photoperiod showed the high speed under the situation of LL (constant light). The eclosion phae of strain managed under LD (periodical light and dark) developed the clear rhythm of eclsion restricted in dark time and realized in light time. The synchronization of its rhythm was most strongly realized right upon the illumination. 2. The mean eclsion time (hours) of five region of natural populations was ranged from 206.6 hours (cheju) to 217.3 hours (Anyang). The eclosion time under LL as faster than DD and LD and male was faster than female. 3. From these resuts, eclsion rhythm in D. melanogaster was synchronized by the circadian cycle under the periodical light and dark environment (12L : 12D).

人工光環境이 금파리(Lucilia caesar)의 發育에 미치는 影響

추종길,김승곤 최신의학사 1977 最新醫學 Vol.20 No.4

The experiments were to analyze the effects on larval developmental time and eclosion rate of Lucilia caesar under artificial light environments. 1. The rate of pupation of Lucilia caesar larvae under the constant light environment (about 2500 lux) was accelerated the developmental time comparing to both environments of constant dark and periodical light and dark environment. 2. The developmental time from 2nd and 3rd instar larvae to adult emergence of Lucilia caesar under the constant light environment was accelerated about 4 or 8 days comparing to another environments.

우리나라 청정지역에서 측정한 PM_(2.5) 입자의 특성

이종훈,김용표,문길주,김희강,정용승,이종범 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

Atmospheric fine particles (PM_(2.5)) were collected at the background sites, Kangwha, Taean, and Kosan and characterized to understand their behaviors at the sites. Daily samples of PM_(2.5) mass were measured and ionic species, carbonaceous species, and gaseous species were analyzed. Four-daγ backward trajectory analysis was also carried out. The mean concentrations of anthropogenic species were highest at Kangwha among three sites, while contributions from sea salts were highest at Taean during the measurement period due to higher wind speed at Taean. Major chemical components in fine particles were sulfates organic carbon, nitrate, and ammonium. Most of the non-sea-salt (nss) sulfates in PM_(2.5) might be present as ammonium sulfates at these sites. Most air parcels arriving at Kangwha and Taean were from northern China. Therefore, both sites were thought to be affected by the same air parcel. At Kosan, during the measurement period, air parcels were from either northern China or southern China. The nss sulfate concentration in the air parcels from southern China was higher, while the nss calcium nitrate, and ammonium concentrations were higher when the air parcels were from northern China.

NP계 비이온 계면활성제/물/용제 시스템의 상거동에 관한 연구

고현길,임종주 동국대학교 산업기술연구원 2000 산업기술논문집 Vol.11 No.2

본 연구에서는 polyoxyethylene nonylphenyl ether(NP) 계의 비이온 계면활성제, 물, 용제의 3성분 계에 대하여 계면활성제, 용제, 온도 등의 변화에 따른 상평형 실험을 수행하였다. 단일상의 microemulsion은 NP-8 시스템의 경우 50℃ 부근의 조건에서 가장 넓은 영역에 걸쳐서 형성되었으며, 계면활성제의 친수성이 증가할수록 저농도의 계면활성제 영역에서 형성되었다. NP series의 계면활성제 시스템에서 생성된 1 phase microemulsion은 bicontinuous microemulsion 보다는 oil-continuous microemulsioin에 가까운 것으로 전기전도도 측정으로부터 확인할 수 있었다. 동일한 계면활성제 시스템을 온도에 따라 비교하였을 때 온도가 증가함에 따라 상전이가 계면활성제의 고농도 방향으로 진행하였으며, 이때 형성된 1 phase microemulsion은 용제 apex 쪽에서 형성되었다. 즉, 비이온 계면활성제의 친수성이 증가할수록 1 phase microemulsion이 생성되는 온도는 증가하며, 계면활성제 농도는 낮아지는 것을 알 수 있었다. 용제로 D-limonene 대신에 YK series의 탄화수소를 사용했을 경우에는 middle-phase microemulsion을 포함한 3 phase 영역이나 1 phase의 microemulsion이 형성되지 않음을 알 수 있었다. Phase behavior for systems containing polyoxyethylene nonylphehyl ether(NP) nonionic surfactant, water, and solvent was studied. One-phase microemulsion was found over a wide range of compositions at 50℃ in NP-8 surfactant system and surfactant concentration necessary for one-phase microemulsion decreased with an increase in surfactant hydrophilicity. Electrical conductivity measurements showed that the one-phase microemulsion formed with NP series nonionic surfactants used during this study was oil-continuous rather than bicontinuous. Increasing temperature shifted the same phase transitions to the direction of increase in surfactant concentration and one-phase microemulsion was formed near the apex of a solvent. It was also found that temperature necessary for one-phase microemulsion increased with an increase in surfactant hydrophilicity. Neither three-phase region containing middle-phase microemulsion nor one-phase microemulsion was found in the same surfactant system except that YK series hydrocarbon solvent was used instead of D-limonene.

초파리 자연집단의 유해유전자 빈도의 변화에 관한 연구

김승곤,추종길,김태전 서울保健大學 1987 論文集 Vol.7 No.1

Drosophila melanogaster의 Hybrid Dysgenesis에 관한 연구 Ⅳ. P-M System 에 의한 한국산 노랑초파리 자연집단의 분석

허미경,추종길 中央大學校 遺傳工學硏究所 1989 遺傳工學硏究論集 Vol.2 No.1

In the P-M system of Hybrid dysgenesis, strains of Drosophila melanogaster can be classfied by sterility tests as P, M and Q. The present paper repots on the distribution of P, M and Q types in the P-M system in Korean natural populations. In 15 strains collected from various sites of korea, five strains were analysed by Q in which they did not produce appreciable gonadal dysgenesis with P males or with M females, and 10 strains were M types. They were examined with respect to the two components of the P-M system, chromosomal P factor and the extrachromosomal cytotypes. The P factors active for potential GD sterility were found at very low frequencies, and the M cytotype which causes susceptility to the P factor is predominant in most strains. But the observation of intermediate and different levels of GD sterility suggests the existance of P factors and cytotype polymorphism in these populations.

地域的差異에 의한 초파리分布와 生態에 關한 硏究

李澤俊,秋鍾吉,羅燾業,姜景來 중앙대학교 기술과학연구소 1975 기술과학연구소 논문집 Vol.2 No.-

Drosophilid flies were collected by the methods of fruit trap and sweeping net from 1969 through 1975 at four different mountains of Korean panisular. 1) Eight genera including 61 species of Drosophilidae were recognized in these survey. During the period of collection, Drosophilid flies were recognized at each mountain as follows; 4 genera, 31 species and 10417 specimens at Mt. Hanla. 4 genera, 22 species and 6537 specimens at Mt. Palkong. 7 genera, 24 species and 4677 specimens at Mt. Unak. 7 genera, 53 species and 9492 specimens at Mt. Sulak. Newly recorded species of Drosophilidae from the results of these survey were as follows; 3 genera, 5 species and 275 specimens at Mt. Hanla. 3 genera, 9 species and 1220 specimens at Mt. Palkong. 7 genera, 24 species and 4677 specimens at Mt. Unak. 3 genera, 8 species and 312 specimens at Mt. Sulak. 1) Altitudinal distribution of Drosophilid flies were analysed at two areas of Mts. Sulak and Hanla. The number of flies decreased at higher altitude, and there were very scarce frequency over 1500m in altitude. However, most flies of S. graminum and D. bifasciata were collected at higher altitude in the season of mid-summer. On the contrary, some species of Drosophilidae, such as D. melanogaster, D. bizonata, D. immigrans and D. lutea showed 1:1 ratio in their sexes. From the results of these survey, the frequency of male flies of D. suzukii express higher than that of opposite sex at low altitude, but ratio showed the same at high in altitude. Some species of Drosophilidae (D. angularis, D. bizonata) collected at field mushroom were not recognized in their 1:1 sex ratio measured from the Chi-spuere fomular. Reason to the factors of these unbalanced ratio of sexes in the natural fields were not known.

우리 나라 배경농도지역의 1996~1997년 미세입자상 탄소성분 측정 결과

김용표,이종훈,문길주,김희강,이종범 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

The concentrations of organic carbon (OC) and elemental carbon (EC) in fine aerosols were determined from the samples collected at the two background sites of Kosan in Cheju Island and Kangwha. Samplings were carried out during the spring and winter of 1996 and the fall and winter of 1997. PM_(2.5) particles were collected on the pre-fired quartz filters for 24 hours and analyzed by the selective thermal oxidation method. The concentrations of OC at the sites were higher than those commonly observed from clean areas around the world, but those of EC at both sites were lower than, or comparable to, other clean areas in the world. Both the OC and EC levels at Kangwha were higher than those at Kosan. According to backward trajectory analysis, most air pollutants collected at thetwo sites were from China. It was found that the OC and EC concentrations in air masses from southern China were higher than those from northern China.