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Jin Hee Eum,Ji-Na Kim,Dong Ryul Lee,Sook Young Yoon 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8
BACKGROUND Ca2+ oscillations during fertilization induce eggs activation and embryonic development in mammalian eggs.. The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is in charge of Ca2+ oscillations for the release of stored Ca2+ from the endoplasmic reticulum. The capacity of this oscillation is obtained during egg maturation and corresponds with an increase in the sensitivity of the IP3R1 and their localization in cytoplasm. Cluster formation of IP3R1 in the egg cortex is important to initiation of Ca2+ oscillations during egg and sperm fusion. In this study, we investigated that cell cycle–coupled redistribution of IP3R1 and Ca2+- oscillatory activity in mouse zygotes. MATERIALS AND METHODS Metaphase II arrested eggs were collected from ICR female mouse after super ovulation induction. At 14 hr post hCG, MII eggs were collected, and artificially activated in Ca2+ free CZB medium with 10 mM SrCl2 for 2 hrs. Pronuclear zygotes (PN) were collected from Strontium activated eggs at 8 hr post activation, and the first mitotic eggs were collected at 16~17 hr post activation. To identify cell cycle coupled IP3R1 redistribution, MII eggs, zygotes, and first mitotic eggs were collected, and fixed for immunostaining with anti-IP3R1antibody (CT-1) and observed on CLSM. Ca2+-oscillatory activity was monitored with fluorescence microscope mounted SimplePCI program (Hamamatsu) after injection of cRNA of mouse phospholipase C zeta (mPLCZ). RESULT IP3R1 were shown clusters, 1~2 um in diameter, in cortex of ovulated MII eggs with high Ca2+ oscillatory activity by mPLCZ injection. These eggs represent more than 6 spikes per 60 min. However, IP3R1 clusters were disappeared in PN eggs and these eggs showed very low Ca2+- oscillatory activity by mPLCZ. In mitosis I stage eggs, clusters of IP3R1 were appeared and Ca2+-oscillatory activity was reactivated slightly (2 spikes per 60 min). CONCLUSIOINS This study introduced the redistribution of IP3R1 clusters were occurred in egg activation according to cell cycle dependent manner. Also, functional modification of IP3R1 including protein phosphorylation was associated with cortical clustering of IP3R1 in cell cycle coupled Ca2+ oscillatory activity.
Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization
Eum, Jin Hee,Park, Miseon,Yoon, Jung Ah,Yoon, Sook Young The Korean Society of Developmental Biology 2020 발생과 생식 Vol.24 No.4
Repetitive changes in the intracellular calcium concentration ([Ca<sup>2+</sup>]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca<sup>2+</sup>]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca<sup>2+</sup> ion elevation during [Ca<sup>2+</sup>]i oscillations are Ca<sup>2+</sup> release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca<sup>2+</sup> ion influx through Ca<sup>2+</sup> channel on the plasma membrane. Ca<sup>2+</sup> channels have been characterized into voltage-dependent Ca<sup>2+</sup> channels (VDCCs), ligand-gated Ca<sup>2+</sup> channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca<sup>2+</sup>]i oscillation was observed in a Ca<sup>2+</sup> contained medium with sperm factor or adenophostin A injection but disappeared in Ca<sup>2+</sup> free medium. Ca<sup>2+</sup> influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca<sup>2+</sup>]i oscillation profiles in SrCl<sub>2</sub> treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca<sup>2+</sup> influx is essential for [Ca<sup>2+</sup>]i oscillation during mammalian fertilization. This Ca<sup>2+</sup> influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.
Clinical outcomes of single versus double blastocyst transfer in fresh and vitrified-warmed cycles
Eum, Jin Hee,Park, Jae Kyun,Kim, So Young,Paek, Soo Kyung,Seok, Hyun Ha,Chang, Eun Mi,Lee, Dong Ryul,Lee, Woo Sik The Korean Society for Reproductive Medicine 2016 Clinical and Experimental Reproductive Medicine Vol.43 No.3
Objective: Assisted reproductive technology has been associated with an increase in multiple pregnancies. The most effective strategy for reducing multiple pregnancies is single embryo transfer. Beginning in October 2015, the National Supporting Program for Infertility in South Korea has limited the number of embryos that can be transferred per in vitro fertilization (IVF) cycle depending on the patient's age. However, little is known regarding the effect of age and number of transferred embryos on the clinical outcomes of Korean patients. Thus, this study was performed to evaluate the effect of the number of transferred blastocysts on clinical outcomes. Methods: This study was carried out in the Fertility Center of CHA Gangnam Medical Center from January 2013 to December 2014. The clinical outcomes of 514 women who underwent the transfer of one or two blastocysts on day 5 after IVF and of 721 women who underwent the transfer of one or two vitrified-warmed blastocysts were analyzed retrospectively. Results: For both fresh and vitrified-warmed cycles, the clinical pregnancy rate and live birth or ongoing pregnancy rate were not significantly different between patients who underwent elective single blastocyst transfer (eSBT) and patients who underwent double blastocyst transfer (DBT), regardless of age. However, the multiple pregnancy rate was significantly lower in the eSBT group than in the DBT group. Conclusion: The clinical outcomes of eSBT and DBT were equivalent, but eSBT had a lower risk of multiple pregnancy and is, therefore, the best option.
Electron Microscopy Investigation at the Initial Growth Stage of Carbon Nanotubes
Sung-Jin Eum,양철웅,박종윤,Hee-Jin Jung,Hee-Kwang Kang,Ji-Beom Yoo 한국물리학회 2003 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.42 No.III
The growth mechanism and the geometry of carbon nanotubes (CNTs) are affected by several parameters such as formation process and catalytic materials. In order to understand the growth mechanism of CNT, it is essential to observe the CNTs at the initial growth stage. We synthesized CNTs directly on the Ni-deposited TEM Cu mesh grid using thermal CVD so as to preserve and investigate the original structure of CNTs from the initial to final growth state without any artifacts that might be introduced during TEM specimen preparation. The results obtained from the electron microscopy study show that the following growth modes of CNTs occur simultaneously at the initial stage: tip-growth, base-growth and intermediate-growth mode. The intermediate growth structure can be achieved by a mixture of base-growth mode and tip-growth mode, which occur sequentially. This is thought to be a probable mechanism for nanotube junctions. The growth mechanism and the geometry of carbon nanotubes (CNTs) are affected by several parameters such as formation process and catalytic materials. In order to understand the growth mechanism of CNT, it is essential to observe the CNTs at the initial growth stage. We synthesized CNTs directly on the Ni-deposited TEM Cu mesh grid using thermal CVD so as to preserve and investigate the original structure of CNTs from the initial to final growth state without any artifacts that might be introduced during TEM specimen preparation. The results obtained from the electron microscopy study show that the following growth modes of CNTs occur simultaneously at the initial stage: tip-growth, base-growth and intermediate-growth mode. The intermediate growth structure can be achieved by a mixture of base-growth mode and tip-growth mode, which occur sequentially. This is thought to be a probable mechanism for nanotube junctions.
Eum, Ji Young,Kim, Yeoung Kyun,Park, Eun Ji,Lee, Ju Hee,Lee, Ji Eun,Lim, Jin Ju,Choi, Man Ho,Kim, Hyun Hee korean Academy of Physical Therapy Rehabilitation 2015 Physical therapy rehabilitation science Vol.4 No.1
Objective: Jump training helps increase the muscle power by improving the muscle strength and reaction time of the muscle in operation. The purpose of this study was to identify the effects of strengthening, stretching exercise and meditation on electromyographic (EMG) onset timing of rectus femoris and gastrocnemius muscle during vertical jump performance. Design: Cross-sectional study. Methods: Ten healthy adults (5 male and 5 female) who were familiar with the vertical jumping task and had no lower extremity injuries or any bone or joint disorders, were recruited for this study. Muscle onset timing was measured by surface EMG. After EMG onset timing were measured during performing three baseline vertical jump trials, strengthening and stretching exercises of the rectus femoris and gastrocnemius, and meditation were performed in random order. EMG onset timing was measured during vertical jump after intervention, respectively. EMG value was averaged for the three trials and analyzed using one-way repeated ANOVA. Results: During vertical jump, EMG onset timing of gastrocnemius was a significant difference after intervention (p<0.05), and then there was significantly faster in strengthening exercise than meditation (p<0.05). Conclusions: These results indicate the potential positive effect of performing strengthening exercise of the gastrocnemius before a jumping event. Future research is required to identify the effects of intervention over a long period.