Effect of gelatinization on the contents of amylose, resistant and total starch with rice varieties

Ji-An Joung,Ji-Young You,Sung-Sil Lee,Joon-Ho Choi 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Identification and characterization of a new isolated Lactobacillus harbinensis from traditionally fermented vinegar

Ji-An Joung,Ji-Young You,Sung-Sil Lee,Joon-Ho Choi 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Antarctic Krill Oil Diet Protects against Lipopolysaccharide-Induced Oxidative Stress, Neuroinflammation and Cognitive Impairment

Choi, Ji Yeon,Jang, Jun Sung,Son, Dong Ju,Im, Hyung-Sik,Kim, Ji Yeong,Park, Joung Eun,Choi, Won Rak,Han, Sang-Bae,Hong, Jin Tae MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12

<P>Oxidative stress and neuroinflammation are implicated in the development and pathogenesis of Alzheimer’s disease (AD). Here, we investigated the anti-inflammatory and antioxidative effects of krill oil. Oil from <I>Euphausia superba</I> (Antarctic krill), an Antarctic marine species, is rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). We examined whether krill oil diet (80 mg/kg/day for one month) prevents amyloidogenesis and cognitive impairment induced by intraperitoneal lipopolysaccharide (LPS) (250 µg/kg, seven times daily) injections in AD mice model and found that krill oil treatment inhibited the LPS-induced memory loss. We also found that krill oil treatment inhibited the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and decreased reactive oxygen species (ROS) and malondialdehyde levels. Krill oil also suppresses IκB degradation as well as p50 and p65 translocation into the nuclei of LPS-injected mice brain cells. In association with the inhibitory effect on neuroinflammation and oxidative stress, krill oil suppressed amyloid beta (1–42) peptide generation by the down-regulating APP and BACE1 expression in vivo. We found that eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (50 and 100 µM) dose-dependently decreased LPS-induced nitric oxide and ROS generation, and COX-2 and iNOS expression as well as nuclear factor-κB activity in cultured microglial BV-2 cells. These results suggest that krill oil ameliorated impairment via anti-inflammatory, antioxidative, and anti-amyloidogenic mechanisms.</P>

전통 발효식초에서 분리한 Lactobacillus harbinensis VF의 프로바이오틱스 활성

정지안 ( Ji An Joung ),유지영 ( Ji-young You ),이성실 ( Sung Sil Lee ),최준호 ( Joon Ho Choi ) 한국산업식품공학회 2023 산업 식품공학 Vol.27 No.2

A novel L. harbinensis VF was isolated from fermented vinegar and identified through biochemical characteristics and 16S rRNA sequencing analysis. Characteristics of probiotics were studied for acid and bile salt tolerances, hemolytic activity, antibiotic resistance, antimicrobial activity, cell surface hydrophobicity, and aggregation. The survival rates of the isolate were maintained at 68.9% and 95.6% after 3 h incubation at pH 2.0 and 2.5 and were over 74% at bile salt concentrations of 0.3% and 0.5%. The hemolytic activity was confirmed to be γ-hemolysis. The isolate showed broad antibiotics-resistance in over 12 antibiotics except for trimethoprim/sulfamethoxazole, compared to L. plantarum (KCTC 3108), used as control, and previously reported L. harbinensis. Antimicrobial activity was confirmed against pathogens, B. cereus, E. coli O157:H7, L. monocytogenes, and S. typhimurium, except S. aureus, and it was attributed to produced organic acids (low pH). The hydrophobicity for xylene and chloroform were 16.7% and 20.4%, respectively, lower than those of L. plantarum (41.2% & 49.9%). The auto-aggregation and co-aggregation with pathogens were 83.8% and 49.2-87.6% at 24 h incubation, respectively, higher than those of L. plantarum. From this study, L. harbinensis VF is likely to be used as a probiotic strain and is a strong candidate for commercial application.

[P226] A case of Darier’s disease

( Min Joung Choi ),( Min Young Lee ),( You Won Choi ),( Hae Young Choi ),( Ji Yeon Byun ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1

Darier’s disease is a genetic disorder of keratinization with autosomal dominant inheritance. The typical warty malodorous hyperkeratotic and crusted skin lesions distribute in seborrheic area of skin. The disease generally appears at a young age, typically by the third decade, and aggravated throughout years. Heat, sweating, friction, and sunlight (ultraviolet light) exacerbate the skin lesion. We report a case of a 64-year-old female patient who presented with yellowish to brownish crusted keratotic papules and plaques with pustules on neck and trunk. Skin lesions lasted more than 30 years and aggravated in the summer. She had hypertension and diabetes history with taking medication. She denied of familial history. Histologic examination revealed parakeratotic hyperkeratosis and suprabasal cleavage with prominent acantholysis. Some dyskeratotic cells, including corps ronds and grains, were presented. The patient’s clinicopathological features were consistent with a diagnosis of Darier’s disease. She treated with oral 20mg/day acitretin. Two weeks later skin lesions much improved and after five weeks hyperketatotic lesion almost cleared. Herein, we report a case of Darier’s disease with very good response to oral acitretin.

국내 허가 및 긴급사용승인된 코로나19 바이러스감염증 치료제의 허가사항 비교 고찰

최민정(Min Joung Choi),서현옥(Hyun Ok Seo),도희정(Hea Jeong Doh),김영림(Younglim Kim),최경숙(Kyoung Suk Choi),박윤주(Younjoo Park),서경원(Kyung Won Seo),정지원(Jeewon Joung),김지연(Ji Yeon Kim),박인숙(In Suk Park),이원재(Wonjae Lee) 대한약학회 2023 약학회지 Vol.67 No.3

In a situation where the development of various treatments is urgently needed to overcome the global outbreak of coronavirus disease 2019 (COVID-19) and the sequential emergence of new variant viruses, Ministry of Food and Drug Safety (MFDS) made tremendous efforts to promptly supply the safe and effective therapeutics manufactured by local and global companies through accelerated marketing authorization or emergency use approvals. To date, seven products (including overlapped) have received marketing authorization or emergency use approvals from MFDS, and their indicated patient group according to the severity, efficacy, and safety information are different by each products. Therefore, this study is to compare the product information and the major clinical outcomes obtained from clinical studies of COVID-19 treatments authorized or approved for emergency use in Korea and to introduce the MFDS’s efforts for establishment of accelerated approval system for COVID-19 treatment and development of relevant guidelines. It is hoped that this study will serve as a reference for the latecomers who is preparing the development of therapeutics for emerging infectious diseases in the future.

Effect of remote ischemic preconditioning on cognitive function after off-pump coronary artery bypass graft:a pilot study

Kyoung-Woon Joung,Jin-Ho Rhim,Ji-Hyun Chin,Wook Jong Kim,Dae Kee Choi,Eun Ho Lee,Kyung-Don Hahm,Ji Yeon Sim,In-Cheol Choi 대한마취통증의학회 2013 Korean Journal of Anesthesiology Vol.65 No.5

Background: Several studies have shown in animal models that remote ischemic preconditioning (rIPC) has a neuroprotective effect. However, a randomized controlled trial in human subjects to investigate the neuroprotective effect of rIPC after cardiac surgery has not yet been reported. Therefore, we performed this pilot study to determine whether rIPC reduced the occurrence of postoperative cognitive dysfunction in patients who underwent off-pump coronary artery bypass graft (OPCAB) surgery. Methods: Seventy patients who underwent OPCAB surgery were assigned to either the control or the rIPC group using a computer-generated randomization table. The application of rIPC consisted of four cycles of 5 min ischemia and 5 min reperfusion on an upper limb using a blood pressure cuff inflating 200 mmHg before coronary artery anastomosis. The cognitive function tests were performed one day before surgery and again on postoperative day 7. We defined postoperative cognitive dysfunction as decreased postoperative test values more than 20% of the baseline values in more than two of the six cognitive function tests that were performed. Results: In the cognitive function tests, there were no significant differences in the results obtained during the preoperative and postoperative periods for all tests and there were no mean differences observed in the preoperative and postoperative scores. The incidences of postoperative cognitive dysfunction in the control and rIPC groups were 28.6% (10 patients) and 31.4% (11 patients), respectively. Conclusions: rIPC did not reduce the incidence of postoperative cognitive dysfunction after OPCAB surgery during the immediate postoperative period.

Ammonium glycyrrhizinate protects gastric epithelial cells from hydrogen peroxide-induced cell death.

Oh, Hyun-Mee,Lee, Sungga,Park, Young-Na,Choi, Eun-Ju,Choi, Ji-Young,Kim, Jeong Ah,Kweon, Ji-Hye,Han, Weon-Cheol,Choi, Suck-Chei,Han, Joung-Kyue,Son, Jong-Keun,Lee, Seung-Ho,Jun, Chang-Duk The Society 2009 Experimental biology and medicine Vol.234 No.3

<P>Glycyrrhiza uralensis has a potential for preventing or ameliorating gastric mucosal ulceration. To understand the molecular mechanism about the medicinal effect of G. uralensis, we isolated four single compounds from G. uralensis and one related compound and screened for the cellular protective effect against H(2)O(2)-induced damage in gastric epithelial AGS cells. Interestingly, we found that ammonium glycyrrhizinate (AG) prepared from glycyrrhizin dramatically protects AGS cells from H(2)O(2)-induced damage as measured by the integrity of actin cytoskeleton. AG also inhibited FeSO(4)-induced reactive oxygen radicals in a dose-dependent manner, suggesting the role for AG as a free radical scavenger. To better understand the protective role of AG at the transcriptional level, we performed genome-wide expression profiling using high-density oligonucleotide microarrays, followed by validation using RT-PCR. Among the 33,096 genes that were screened in the microarray, 936 genes were found to be differentially expressed in a statistically significant manner in the presence or absence of H(2)O(2) and AG. Among the 936 genes, 51 genes were differentially expressed at least 3-fold in response to the H(2)O(2) treatment. AG blocked the expression of genes related to apoptotic cell death (GDF15, ATF3, TNFRSF10A, NALP1) or oxidative stress path-ways (HMOX1) which was elevated in response to H(2)O(2) treatment, suggesting a potential protective role for AG in oxidative stress-induced cell death. Collectively, current results demonstrate that AG is a novel antioxidant that could be effective for the treatment of gastric diseases related to the oxidative stress-induced mucosal damage.</P>

Mixed lactic acid fermentation with L. harbinensis VF and L. plantrum using non-waxy rice

Ji Young You,Ji An Joung,Joon Ho Choi 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

We investigated the rice-based lactic acid fermentation with L. plantarum (LP) and L. harbinensis VF (LH) because rice is known as carbohydrate-rich and gluten-free food. Non-waxy rice flour was liquefied and then saccharified using AMG 300L. From the previous study on medium optimization, the concentrations of rice flour and yeast extract for lactic acid fermentation were determined to be 3% and 1% (w/v). By replacing glucose with pre-treated rice flour, the specific growth rates of LP and LH increased from 0.18 and 0.16 to 0.33 and 0.21 h<SUP>-1</SUP>, respectively. The maximum cell concentrations reached 1.99×10<SUP>8</SUP>, 8.54×10<SUP>7</SUP>, 2.32×10<SUP>8</SUP> CFU/mL with LP, LH, and mixed fermentation, respectively. In LH fermentation, the lactic and acetic acids produced 90.9±0.3 mM and 15.7±0.1 mM, which is better than those in LP and mixed fermentation. The lactic acid fermentation with LH provides a buttery flavor, one characteristic of yogurt. In the lactic acid fermentation with non-waxy rice, VF is a good candidate for metabolites and flavor, whereas LP and mixed LP+LH for cell concentration. This study may provide the guideline in lactic acid fermentation with rice.

Heparin-Conjugated Pluronic Nanogels as Multi-Drug Nanocarriers for Combination Chemotherapy

Joung, Yoon Ki,Jang, Ji Young,Choi, Jong Hoon,Han, Dong Keun,Park, Ki Dong American Chemical Society 2013 Molecular pharmaceutics Vol.10 No.2

<P>Combination chemotherapy using more than two therapeutic agents with different modes of action is a promising strategy that can be used to enhance the therapeutic efficacy of cancer treatment, even though it is a complicated treatment modality. The aim of this study was to investigate how a novel multidrug nanocarrier is effective for combination chemotherapy <I>in vitro</I> and, more specifically, whether combined agents with different modes of action and physicochemical properties show synergistic cytotoxicity with the use of this nanocarrier. A heparin–Pluronic (Hep–Pr) nanogel encapsulating both paclitaxel and DNase was shown to be efficient for intracellular delivery with respect to size, encapsulation efficiency, and intracellular uptake/fates. As a result of these properties, a Hep–Pr nanogel combined with paclitaxel and DNase exhibited a dose-dependent synergistic cytotoxicity compared to single drug and free-drug treatments, whose combination indices were 0.93 and 0.45 at higher concentrations (250 and 500 μg/mL). Therefore, Hep–Pr nanogels have the potential to deliver multitherapeutic agents with different characteristics and thereby enhance the therapeutic efficacy of combination cancer chemotherapy.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mpohbp/2013/mpohbp.2013.10.issue-2/mp300480v/production/images/medium/mp-2012-00480v_0010.gif'></P>