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Jung, Kwang Bo,Lee, Hana,Son, Ye Seul,Lee, Ji Hye,Cho, Hyun-Soo,Lee, Mi-Ok,Oh, Jung-Hwa,Lee, Jaemin,Kim, Seokho,Jung, Cho-Rok,Kim, Janghwan,Son, Mi-Young Federation of American Societies for Experimental 2018 The FASEB Journal Vol. No.
<P>Human intestinal organoids (hIOs) derived from human pluripotent stem cells (hPSCs) have immense potential as a source of intestines. Therefore, an efficient system is needed for visualizing the stage of intestinal differentiation and further identifying hIOs derived from hPSCs. Here, 2 fluorescent biosensors were developed based on human induced pluripotent stem cell (hiPSC) lines that stably expressed fluorescent reporters driven by intestine-specific gene promoters Kruppel-like factor 5 monomeric Cherry (KLF5(mCherry)) and intestine-specific homeobox enhanced green fluorescence protein (ISXeGFP). Then hIOs were efficiently induced from those transgenic hiPSC lines in which mCherry- or eGFP-expressing cells, which appeared during differentiation, could be identified in intact living cells in real time. Reporter gene expression had no adverse effects on differentiation into hIOs and proliferation. Using our reporter system to screen for hIO differentiation factors, we identified DMH1 as an efficient substitute for Noggin. Transplanted hIOs under the kidney capsule were tracked with fluorescence imaging(FLI) and confirmed histologically. After orthotopic transplantation, the localization of the hIOs in the small intestine could be accurately visualized using FLI. Our study establishes a selective system for monitoring the in vitro differentiation and for tracking the in vivo localization of hIOs and contributes to further improvement of cell-based therapies and preclinical screenings in the intestinal field.</P>
Oh, Jung-Hwa,Jung, Cho-Rok,Lee, Mi-Ok,Kim, Janghwan,Son, Mi-Young UNKNOWN 2018 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.41 No.2
<P>Alternative cell models of human neural stem cells (hNSCs) have been developed and used for investigations ranging from <I>in vitro</I> experiments to <I>in vivo</I> clinical studies. However, a cell model capable of mimicking the ʻnormalʼ state of hNSCs is mandatory in order to extrapolate the results of these studies to humans. In the present study, to select a more suitable hNSC model for developing human-based experimental platforms, two representative hNSC types were compared, namely human embryonic stem cell (hESC)-derived hNSCs and ReNcell CX cells, which are well-characterized immortalized hNSC lines. The hNSCs, differentiated from hESCs via human neuroectodermal sphere (hNES) formation, recapitulated the molecular and cellular phenotypes of hNSCs, including NSC marker expression and terminal neuronal differentiation potential. Comparative analyses of the transcriptome profiles of the hESC-derived hNESs and ReNcell CX hNSCs showed that the differentiated hNESs were analogous to the ReNcell CX cells, as demonstrated by principal component analysis and hierarchical sample clustering. The hNSC-specific transcriptome was presented, comprising commonly expressed transcripts between hNESs derived from hESCs and ReNcell CX cells. To elucidate the molecular mechanisms associated with the hNSC identity, the hNSC-specific transcriptome was analyzed using pathway and functional annotation clustering analyses. The results suggested that hESC-derived hNESs, an expandable and accessible cell source, may be used as a relevant hNSC model in a wide range of neurological investigations.</P>
Automatic UML Design Extraction with Software Visualization based on Reverse Engineering
Jung, Se Jun,Kim, Janghwan,Lee, Won Young,Park, Bo Kyung,Son, Hyun Seung,Kim, R. Young Chul The Institute of Internet 2021 International journal of advanced smart convergenc Vol.10 No.3
In various areas of the 4th industry, a big issue is software quality enhancement for stability and reliability of the smart software systems. After revising software promotion law at 2020, we must clearly define requirements and separate design parts and implementation parts of an all public software development contracts. In this study, we need to validate whether the final implementation of software is followed by the original design or not. To do this, we consider the design restoration through software visualization based on reverse engineering. Therefore we propose an UML design extraction and visualization method based on reverse engineering. Based on this, we may validate whether it is implemented according to the original design, and how much visualizes and includes the code the internal complexity for improvement of software quality.
Chae, Jung-Il,Kim, Janghwan,Woo, Sun-Mi,Han, Hyo-Won,Cho, Young Keun,Oh, Keon-Bong,Nam, Ki-Hoan,Kang, Yong-Kook WILEY-VCH Verlag 2009 Proteomics Vol.9 No.5
<P>The ability to generate neural lineages from human embryonic stem cells (hESCs) in a controlled manner would further investigation of human neurogenesis and development of potential cell therapeutic applications to treat neurological diseases; however, generating such neural stem cells (NSCs) remains a challenge. In an attempt to characterize the cellular mechanisms involved in hESC differentiation into neuroprogenitor cells, we performed 2-DE using protein extracts from hESC-derived embryoid bodies (EBs) and neuroectodermal spheres (NESs) bearing neuroprogenitors. Of 47 differentially expressed protein spots, 28 nonredundant spots were shown to be upregulated in the NESs; these protein spots included neurogenesis-related proteins (TAF1, SEPT2, NPH3, and CRABP), as expected. Interestingly, 6 of these 28 protein spots were cytoskeleton-associated proteins (CSAP) such as Fascin-1, Cofilin-1, and Stathmin-1. Western-blot analyses confirmed the increased levels of these proteins in the NESs. Furthermore, immunostaining analysis showed that both Fascin-1 and Stathmin-1 were preferentially expressed in the inner rims of neural rosettes, which are characteristic features of neuroprogenitors in culture. We also confirmed prominent expression of Fascin-1 in (sub-)ventricular zone in E15.5 mouse fetal brain. Our results suggest that, in addition to the induction of those genes involved in neural development, hESC differentiation into the NES is associated with a marked reorganization of the cellular cytoskeleton.</P>