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Lyu,Jae-Il,Lee,Sun-Ha,Lim,Keun-Chul,Kim,Gil-Ja,Yang,Deok-Chun,Bae,Chang-Hyu 한국자원식물학회 2003 Plant Resources Vol.6 No.3
Genetic diversity of 45 tea accessions from Korea, Japan, China and Taiwan was investigated by using RAPD analysis. Out of the eighty primers screened, twenty primers generated 99 polymorphic bands with a polymorphic rate 87.0%. The size of the amplified fragments ranged from about 3,138 bp to 520 bp. By cluster analysis, all of the 45 accessions can be grouped into five groups. Over 90% of the 32 Korean accessions belonged to group II, III, IV and V. Moreover, newly developed Korean cultivars (accession no. 13, 14 and 15) belonged to very different group compared with any other Korean accessions. Among the Korean accessions, the minimum genetic similarity 0.500 was obtained between accession no. 17 and 37 and the largest genetic similarity 0.912 between no. 20 and 21.
[PG-0003] Phytochemical compounds and antioxidant activates of Hop (Humulus lupulus L.) cultivars
Jae Il Lyu(Jae Il Lyu ),Jaihyunk Ryu(Jaihyunk Ryu ),Tae Hyun Ha(Tae Hyun Ha ),Jun-Hyung Lee(Jun-Hyung Lee ),Sang Hoon Park(Sang Hoon Park ),Hee Kyoung Kang(Hee Kyoung Kang ),Joon-Woo Ahn(Joon-Woo Ahn 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Phenolic Compound Content of Leaf Extracts from Different Roselle (Hibiscus sabdariffa) Accessions
( Jae Il Lyu ),( Jung Min Kim ),( Dong-gun Kim ),( Jin-baek Kim ),( Sang Hoon Kim ),( Joon-woo Ahn ),( Si-yong Kang ),( Jaihyunk Ryu ),( Soon-jae Kwon ) 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.1
The leaves of roselle (Hibiscus sabdariffa L.) have been used as a traditional folk medicine that has diuretic and mild laxative effects. Roselle is cultivated in many countries for medicines and food. However, studies on the variation of functional compounds in different accessions are relatively limited. In this study, we investigated the phenolic compound content of leaf extracts from 49 different roselle accessions from a worldwide collection by ultra-high performance liquid chromatography mass spectrometry. The aim was to chemically classify and assess the pharmacological properties of the phenolic compounds in the leaves. The phenolic compounds were identified as neochlorogenic acid, cryptochlorogenic acid, rutin, rutin isomer, isoquercitin, kaempferol-3-O-rutinoside, kaempferol-3-O-glucoside, quercetin, quercetin isomer, and kaempferol. These compounds were present at significantly different levels among the accessions. The most abundant phenolic compound was rutin, which was found in 45 accessions where the total phenolic compound content was 18.75-46.51 mg/g (average 31.52 mg/g). The phenolic compounds were classified into four groups. The two accessions with the highest total phenolic compound content were from Ghana (PI286316) and Senegal (PI275413). The hierarchical cluster analysis of the 49 roselle accessions showed that they formed five groups according to their phenolic compound content. Our results will be useful for the selection of roselle genotypes with improved functional compounds.
Jae Il Lyu,최홍일,Jaihyunk Ryu,Soon‑Jae Kwon,Yeong Deuk Jo,Min Jeong Hong,Jin‑Baek Kim,Joon‑Woo Ahn,Si‑Yong Kang 한국식물학회 2020 Journal of Plant Biology Vol.63 No.1
Kenaf is a valuable fber and medicinal crop. Here, we performed RNA-sequencing to obtain comprehensive transcriptome information and identify putative genes involved in biosynthetic pathways of anthocyanins and kaempferitrin in kenaf. A total of 39.6 Gb reads were generated for six kenaf accessions. Individual de novo assembly of each accession and clustering of whole transcriptome contigs fnally yielded 299,880 representative transcripts having an average length of 1217 bp, of which 231,825 (77.3%) were annotated against various databases. Expression profling of the transcripts identifed 6,592 diferentially expressed genes (DEGs) among the three accessions, C-14 (a local landrace) and two of its mutant varieties, Jeokbong (purple leaves and stems) and Baekma (light green leaves and stems). In addition to their altered coloration, leaves of these two mutants had signifcantly diferent anthocyanin and kaempferitrin contents. We constructed anthocyanin and kaempferitrin biosynthetic pathways and identifed 671 transcripts mapping to the entire pathway. In addition, 29 DEGs assigned to eight structural genes and 41 DEGs related to three transcription factor families were identifed in high anthocyanin- and kaempferitrin-accumulating mutant Jeokbong. Our results provide a large transcriptome library pool for understanding gene functions and should also be useful in further studies of favonoid biosynthesis in kenaf.
류재일(Jae Il Lyu),이선하(Seon Ha Lee),배창휴(Chang Hyu Bae) 한국차학회 2005 한국차학회지 Vol.11 No.3
차 수집종 집단의 분자유전학적 특성을 검토하기 위하여 한국 수집봉 32게통, 대만종 3품종, 중국종 4품중, 일본종 6품종, 총 45 수집계통에 대하여 AFLP분석을 수행하였다. AFLP 분석결과 총 DNA 밴드수 382개 중 87.7%가 다형성을 나타냈다. 총 45 수집계통 간의 유사도지수는 0.05~1.00 범위였고, 이 중 한국수집계통간의 유사도지수는 0.10~0.95로 나타났다. 군집분석 결과 총 45 수집계통이 6개 그룹으로 나눠졌다. 한국수집계통은 제6그룹을 제외한 나머지 그룹에 모두 분포하였으며, 이 중 3, 4, 5그룹은 한국수집종 만으로 구성되었다. 이와 같이 AFLP분석을 통하여 본 연구에서 사용된 한국 수집계통은 높은 유전적 다양성을 가지고 있었으며, 이 수집 차나무집단은 차의 품종육성을 위한 유전자원으로서 활용이 기대된다. Amplified fragment length polymorphism (AFLP) analysis was examined to detect genetic characteristics among 45 accessions of tea(Camellia sinensis var. sinensis (L.) O. Kuntze) from Korea, Japan, China and Taiwan. As a result of AFLP analysis, a total of 382 DNA bands were scored and 335 were polymorphic with a polymorphic rate 87.7%. Genetic similarity shows very different from 0.05 to 1.00 among the 45 accessions and 0.10 to 0.95 among the Korean accessions. By cluster analysis, the 45 accessions were grouped into six. Korean accessions that belonged to group 3.4 and 5 did not have Japanese, Chinese and Taiwanese varieties, The results indicate that the Korean accessions as well as 45 accessions have high genetic diversity, thus the Korean tea accessions serve as basic gene pool in tea breeding.