Development and characterization of ten polymorphic microsatellite markers in the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae)

Min Jee Kim,Ki-Hwan Kim,Kee Young Kim,Iksoo Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

The seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae) has a broad ecological range, living almost anywhere there are aphids for feeding. In this study, we isolated and characterized a total of 10 microsatellite loci from the species. The loci were validated and characterized using 25 samples collected from five Korean localities. The number of alleles and heterozygosity observed at each locus ranged from 4 to 16 and from 0.37 to 0.89, respectively. None of the loci deviated significantly from Hardy-Weinberg equilibrium and there was no indication of significant linkage disequilibrium among pairs of loci. These microsatellite markers should be very valuable markers for population genetic studies of Coccinella septempunctata.

Mitogenome descriptions of three new beetles belonging to Scarabaeiformia (Insecta: Coleoptera): Genomic comparison and phylogenetic analysis in Coleoptera

Min Jee Kim,Man Il Kim,Ki Gyoung Kim,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

Up to now only 17 coleopteran mitogenomes have been fully or near fully sequenced, but this chart does not include any complete mitogenome sequence of the coleopteran infraorder Scarabaeiformia, in which the monotypic Scarabaeoidea is included. In this study, we firstly present three scarabaeoid mitogenomes, two of which are completed and one is near completed. The complete nucleotide sequences of the white-spotted flower chafer, Protaetia brevitarsis (Scarabaeiformia: Scarabaeidae), was determined to be 20,319 bp, the two-spotted stag beetle, Metopodontus blanchardi (Scarabaeiformia: Lucanidae), was 21,678 bp, and the incomplete garden chafer, Polyphylla laticollis (Scarabaeiformia: Scarabaeidae), which lacks sequence information from whole A+T-rich region and a partial srRNA gene was 14,473 bp. Thus, the two complete mitogenome sequences are longest among the completely sequenced insect mitogenomes, followed by the 19,517 bp-long dipteran Drosophila melanogaster. Phylogenetic analysis using the concatenated 13 amino acid sequences of PCGs of the 13 coleopteran insects including three newly sequenced scarabaeoid successfully recovered a monophyly of suborder Polyphaga, a monophyly of infraorder Cucujiformia, a monophyly of infraorder Elateriformia, and also a monophyly of infraorder Scarabaeiformia within Polyphga. However, the Scarabaeiformia represented by three newly sequenced species was unexpectedly placed as a sister group to the Cucujiformia, instead of the placement to the sister to the Cucujiformia and Elateriformia group.

Optical parameters of boron-doped ZnO nanorods grown by low-temperature hydrothermal reaction.

Kim, Soaram,Park, Hyunggil,Nam, Giwoong,Yoon, Hyunsik,Kim, Younggyu,Kim, Byunggu,Ji, Iksoo,Kim, Jong Su,Kim, Jin Soo,Kim, Do Yeob,Kim, Sung-O,Leem, Jae-Young American Scientific Publishers 2014 Journal of Nanoscience and Nanotechnology Vol.14 No.11

<P>Sol-gel spin-coating was used to deposit ZnO seed layers onto quartz substrates, and ZnO nanorods doped with various concentrations of B (i.e., BZO nanorods) ranging from 0 to 2.0 at% were hydrothermally grown on the ZnO seed layers. The effects of B doping on the absorption coefficient, optical band gap, Urbach energy, refractive index, extinction coefficient, single-oscillator energy, dispersion energy, average oscillator strength, average oscillator wavelength, dielectric constant, and optical conductivity of the hydrothermally grown BZO nanorods were investigated. The optical band gaps were 3.255, 3.243, 3.254, 3.258, and 3.228 eV for the nanorods grwon at 0, 0.5, 1.0, 1.5 and 2.0 at% B, respectively. B doping increased the Urbach energy from 40.7 to 65.1 meV for the nanorods grown at 0 and 2.0 at% B, respectively, and significantly affected the dispersion energy, the single-oscillator energy, the average oscillator wavelength, the average oscillator strength, the refractive index, and the optical conductivity of the hydrothermally grown BZO nanorods.</P>

Geographic Genetic Contour of the Dung Beetle, Copris tripartitus (Coleoptera: Scarabaeidae), that Is Endangered in Korea

Ki-Gyoung Kim,Ah Rang Kang,Min Jee Kim,Iksoo Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

The dung beetle, Copris tripartitus, (Coleoptera: Scarabaeidae), is one of the insect species listed as endangered wildlife in Korea. In order to establish conservation strategy an investigation on nation-wide genetic magnitude and nature of genetic diversity would be required. In this study, we sequenced each partial sequences of mitochondrial COI (658 bp) and CytB (433 bop) genes and complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA (411~420 bp) from 70 individuals of C. tripartitus collected from five Korean localities. The sequence divergence of mitochondrial genes was unexpectedly substantial as 5.0% (33 bp) for COI and 4.6% (20 bp) for CytB, whereas that of ITS2 was only 1.9% (6 bp), revealing much higher variability in mitochondrial DNA. In phylogenetic analysis, each 57 and 47 haplotypes obtained from COI and CytB gene sequences was subdivided into two groups (groups A and B), but the node supports for each group was not strong enough to consider each group as independent monophyletic groups in both genes. This interpretation was further supported by the same analysis with ITS2, showing no subdivision at all. These findings reinforce importance for the inference of phylogenetic result using both mitochondrial and nuclear encoded sequences. The C. tripartitus occurring in Korean peninsula was genetically well connected to each other and no obvious population genetic structure was detectable.

Complete Mitochondrial Genome Sequence of the Yellow-spotted Long-horned Beetle Psacothea hilaris (Coleoptera: Cerambycidae) and Phylogenetic Analysis among Coleopteran Insects

Ki Gyoung Kim,Mee Yeon Hong,Min Jee Kim,Man Il Kim,Chang Hwan Bae,Sook Jae Seo,Sang Hyun Lee,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

We have determined the complete mitochondrial genome of the yellow-spotted long horned beetle, Psacothea hilaris (Coleoptera: Cerambycidae), an endangered insect species in Korea. The 15,856-bp long P. hilaris mitogenome harbors gene content typical of the animal mitogenome and a gene arrangement identical to the most common type found in insect mitogenomes. As with all other sequenced coleopteran species, the 5-bp long TAGTA motif was also detected in the intergenic space sequence located between tRNASer (UCN) and ND1 of P. hilaris. The 1,190-bp long non-coding A+T-rich region harbors an unusual series of seven identical repeat sequences of 57-bp in length and several stretches of sequences with the potential to form stem-and-loop structures. Furthermore, it contains one tRNAArg-like sequence and one tRNALys-likes equence. Phylogenetic analysis among available coleopteran mitogenomes using the concatenated amino acid sequences of PCGs appear to support the sister group relationship of the suborder Polyphaga to all remaining suborders, including Adephaga, Myxophaga, and Archostemata. Among the two available infraorders in Polyphaga, a monophyletic Cucujiformia was confirmed, with the placement of Cleroidea as the basal lineage for Cucujiformia. On the other hand, the infraorder Elateriformia was not identified as monophyletic, thereby indicating that Scirtoidea and Buprestoidea are the basal lineages for Cucujiformia and the remaining Elateriformia.

Effects of Ga concentration on the structural, electrical and optical properties of Ga-doped ZnO thin films grown by sol-gel method

Yoon, Hyunsik,Kim, Soaram,Park, Hyunggil,Nam, Giwoong,Kim, Yangsoo,Leem, Jae-Young,Kim, Min Su,Kim, Byunggu,Kim, Younggyu,Ji, Iksoo,Park, Youngbin,Kim, Ikhyun,Lee, Sang-heon,Jung, Jae Hak,Kim, Jin Soo Korean Physical Society 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.1

Undoped ZnO and Ga-doped ZnO (GZO) thin films with different Ga concentrations were prepared by using the sol-gel spin-coating method. The surface morphologies and the growth orientations of the films were measured by using scanning electron microscopy and X-ray diffraction, respectively. The electrical properties were measured by using the Hall effect. The optical transmittances and reflectances of the films were measured as functions of the wavelength by UV-vis spectroscopy. The undoped ZnO thin films exhibited rough surfaces with particle-like structures. When Ga was incorporated, the particle sizes dramatically decreased without changes in the surface morphologies, and the c-axis growth orientations of the GZO thin films were significantly decreased. The optical transmittances clearly exhibited shifts in the band edge, and those in the visible range gradually increased with increasing Ga concentration. The absorption coefficients, refractive indices, extinction constants, dielectric constants, and optical conductivities of the films gradually decreased with increasing Ga concentration.

Complete Nucleotide Sequence and Organization of the Mitogenome of the Endangered Eumenis autonoe (Lepidoptera: Nymphalidae) and Phylogenetic Analysis among Lepidopteran Insects

Min Jee Kim,Man Il Kim,Ah Rang Kang,Ki Gyoung Kim,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Eumenis autonoe belonging to a lepidopteran family Nymphalidae (superfamily Papilionoidea) is an endangered species in Korea. Historically, the species was distributed in Europe and Asian region including a wide region in Korean peninsula. However, in Korean peninsula, the species is found only in two isolate dregions: South in a remote island Jeju, where altitude is higher than1, 400 meter on Halla Mt. and North in far northern Korean peninsula around Mt. Bekdu. In this study, we report the complete mitochondrial genome of the endangered E. autonoe collected from Mt. Halla. The 15,489-bp long E. autonoe genome has a typical gene content found in animal mitochondrial genomes and contains the gene arrangement identical to all other sequenced lepidopteran insects, which differs from the most common type found in insects, as the result of the movement of tRNAMet to a position 5’-upstream of tRNAIle. As seen in many other lepidopteran insects, no typical ATN codon for COI gene is available. Thus, we tentatively designated the CGA (arginine) found at the beginning of the COI gene, as has been suggested for lepidopteran COI starter. The intergenic spacer sequence located between tRNASer (UCN) and ND1 of E. autonoe mitogenome also contains the ATACTAA motif which is conserved in all sequenced lepidopteran species. The 678-bp long A+T-rich region, which is longest in sequenced lepidopteran insects contains ten identical tandem repeats composed of 27 bp plus one 13-bp long identical incomplete final repeat. Such repeat sequence is rare in the lepidopteran mitogenomes known so far. The E. autonoe A+T-rich region also contains a poly-T stretch located at the end of the region as 19 bp and also contains the downstream conserved motif ATAGA that were previously suggested to serve as a structural signal for minor-strand mtDNA replication. Phylogenetic analysis using the concatenated 13 amino acid sequences of PCGs among available six lepidopteran superfamilies (Tortricoidea, Pyraloidea, Papilionoidea, Bombycoidea, Geometroidea, and Noctuoidea) rooted with three dipteran species with BI and ML analyses supported the following topology: ((((Bombycoidea + Geometroidea +Noctuoidea) + Papilionoidea) + Pyraloidea) + Tortricoidea). Within Papilionoidea, a closer relationship between Lycaenidae and Pieridae, excluding Nymphalidae was observed. Further fruitful information will be available after more analysis is done.

Genetic Relationships between Oeneis urda and O. mongolica (Nymphalidae: Lepidoptera)

Sung-Soo Kim,Xinlong Wan,Min Jee Kim,Iksoo Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

The species status of Oeneis urda (Eversmann) and O. mongolica (Oberthür) has been argued based on morphological characters. Reexamination of their major morphological characters has shown a slight differentiation in the two species. Sequences of three mitochondrial genes (COI, ND6, and ND1) and one nuclear region (internal transcribed spacer 2, ITS2) from two O. urda populations (Yangyang and Mt. Hanla) and one O. mongolica population (Uljin) were performed for phylogenetic and population genetic inferences. Sharing of identical sequences in the ND6 gene and ITS2, minimal sequence divergence in the COI and ND1 genes, and phylogenetically undividable sequence types in all mitochondrial genes and ITS2 suggest genetic continuity between the two species. Nevertheless, significant FST estimates (p < 0.05) were found for the COI gene in comparisons between Yangyang (O. urda) and Uljin (O. mongolica), between Yangyang (O. urda) and Mt. Hanla (O. urda), and between Uljin (O. mongolica) and Mt. Hanla (O. urda) populations. These FST estimates, along with other gene-based analyses collectively suggest isolation of the two species at some point in the past, but incomplete separation between the two species on the mainland (Yangyang and Uljin) and biogeographically forced isolation of the O. urda population on Mt. Hanla collectively appear to complicate species status of these two species that were once further clearly separated.

Saturnia jonasii Butler, 1877 on Jejudo Island, a new saturnid moth of South Korea with DNA data and morphology (Lepidoptera: Saturniidae)

Min Jee Kim,Sung-Soo Kim,Sei-Woong Choi,Iksoo Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

Saturnia (Rinaca) jonasii Butler, 1877 is distributed in Japan, including Tsushima Island and Taiwan, whereas S. boisduvalii Eversmann, 1846 is distributed in northern areas, such as China, Russia, and South Korea. In the present study we found that the specimens from Mt. Hallasan on Jejudo, a southern remote offshore island, were S. jonasii, rather than S. boisduvalii based on morphology, DNA barcode, and nuclear elongation factor 1 alpha (EF-1α) sequences. The major morphological differences between the two species included the shape of wing pattern elements of fore- and hindwings and male and female genitalia. A DNA barcode analysis of the sequences of the Jejudo specimens and S. boisduvalii, along with those of Saturnia species obtained from a public database showed a minimum sequence divergence of 4.26% (28 bp). A phylogenetic analysis also showed clustering of the Jejudo specimens with S. jonasii, separating S. boisduvalii (Bayesian posterior probability = 0.99). The EF-1α-based sequence and phylogenetic analyses of the two species from Jejudo Island and the Korean mainland showed the uniqueness of the Jejudo specimens from S. boisduvalii collected on the Korean mainland, indicating distribution of S. jonasii on Jejudo Island in South Korea, instead of S. boisduvalii.

Molecular phylogeny of Drosophila with the description of newly sequenced mitochondrial genome of the spotted-wing drosophila D. suzukii (Diptera: Drosophilidae)

Jong Seok Kim,Min Jee Kim,Deuk-Soo Choi,Iksoo Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

The spotted-wing drosophila Drosophila suzukii (Diptera: Drosophilidae) is an Asian species introduced into North America and Europe. It damages a wide variety of thin-skinned fruits. We sequenced the complete mitochondrial genome (mitogenome) of D. suzukii to better understand the mitogenomic characteristics of this species and understand phylogentic relationships of Drosophila. The 16,230-bp complete mitogenome of the species consists of a typical set of genes, including 13 protein-coding genes (PCGs), two rRNA genes, and 22 tRNA genes, and one major non-coding A+T-rich region, with an arrangement typical of insects. Twelve PCGs began with the typical ATN codon, whereas the COI began with TCG, which has been designated as the start codon for other Drosophila species. The 1,525-bp A+T-rich region is the second longest in Drosophila species for which the whole mitogenome has been sequenced, after D. melanogaster. Phylogenetic analysis with the 13 PCGs of the Drosophila species using Bayesian Inference and Maximum likelihood methods both placed D. suzukii at the basal lineage of the previously defined Melanogaster group, with a strong support.