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- 저자 : ( Hyunwoo Namgung ) (검색결과 3 건)
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Graphene Oxide–PolyethylenimineNanoconstructas a Gene Delivery Vector and Bioimaging Tool
Kim, Hyunwoo,Namgung, Ran,Singha, Kaushik,Oh, Il-Kwon,Kim, Won Jong American ChemicalSociety 2011 Bioconjugate chemistry Vol.22 No.12
<P>Graphene oxide (GO) has attracted an increasing amountof interestbecause of its potential applications in biomedical fields such asbiological imaging, molecular imaging, drug/gene delivery, and cancertherapy. Moreover, GO could be fabricated by modifying its functionalgroups to impart specific functional or structural attributes. Thisstudy demonstrated the development of a GO-based efficient gene deliverycarrier through installation of polyethylenimine, a cationic polymer,which has been widely used as a nonviral gene delivery vector. Itwas revealed that a hybrid gene carrier fabricated by conjugationof low-molecular weight branched polyethylenimine (BPEI) to GO increasedthe effective molecular weight of BPEI and consequently improved DNAbinding and condensation and transfection efficiency. Furthermore,this hybrid material facilitated sensing and bioimaging because ofits tunable and intrinsic electrical and optical properties. Consideringthe extremely high transfection efficiency comparable to that of high-molecularweight BPEI, high cell viability, and its application as a bioimagingagent, the BPEI–GO hybrid material could be extended to siRNAdelivery and photothermal therapy.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bcches/2011/bcches.2011.22.issue-12/bc200397j/production/images/medium/bc-2011-00397j_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bc200397j'>ACS Electronic Supporting Info</A></P>
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Effect of [EMIM]Ac amount remained in the pretreated lignocellulose on enzymatic saccharification
( Songyi Han ),( Chanwoo Park ),( Hyunwoo Namgung ),( Pureunnarae Seo ),( Seunghwan Lee ) 한국목재공학회 2016 한국목재공학회 학술발표논문집 Vol.2016 No.2
The pretreatment to open up or disrupt the cell wall structure of lignocellulosic biomass is an essential process to improve the enzymatic saccharification. Ionic liquids have been used as new green solvents for the pretreatment of lignocellulosic biomass to improve enzymatic saccharification. l-Eethyl-3-methyl imidazolium acetate ([EMIM]Ac) is one of the best ionic liquids for the enzymatic saccharification of lignocellulosic biomass. However, [EMIM]Ac is known to inhibit the activity of enzyme and difficult to be completely removed from the pretreated product. Thus, this study evaluated the effect of the [EMIM]Ac amount remained in the pretreated lignocellulose on enzymatic saccharification by adjusting its amount during washing process with distilled water after pretreatment. The pussy willow (Salix gracilistyla Miq.) was used as lignocellulosic biomass and enzyme was performed using enzyme cocktail with acremonium-derived enzyme (Meiji Seika Co., Tokyo, Japan) and optimash BG (Genencor, Rochester, New york, USA) at 45℃ for 72 hours using shaking incubator.
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Pretreatment of lignocellulose using recycled ionic liquid for enzymatic saccharification
( Songyi Han ),( Soyeon Kim ),( Seonngmin Ji ),( Chanwoo Park ),( Hyunwoo Namgung ),( Pureunnarae Seo ),( Seunghwan Lee ) 한국목재공학회 2016 한국목재공학회 학술발표논문집 Vol.2016 No.2
Ionic liquids is known to have many merits in view of green chemistry, such as high thermal stability, non-volatility, non-flammability, low melting points, excellent dissolution ability compared with volatile organic solvents. With these advantages, ionic liquids have been used as new green solvents in various biorefinery research fields, especially for the pretreatment of lignocellulosic biomass to improve enzymatic saccharification. Especially, 1-ethyl-3-methyl imidazolium acetate ([EMIM]Ac) has been known to be effective for the dissolution of total lignocellulose components. However, ionic liquids are generally more expensive than other organic solvents, which remains a problem to be solved. This study was carried out to investigate how may times [EMIM]Ac) can be reused for the pretreatment, not losing its effectiveness.
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