生物學的 窒素·隣除去에 關한 硏究

金元滿,柳亨烈 漢陽大學校 環境科學硏究所 1991 環境科學論文集 Vol.12 No.-

富營養化(Eutrophication) 影響物質인 窒素와 燐의 除去方法中 N·P 除去를 위한 生物學的高度處理方法中의 하나인 A₂/O 工法(Anaerobic Anoxic/Oxic Process)에서 除去效率增大를 위해 사용하는 메탄올의 경제적 대처방안의 하나로 一次沈澱池의 沈澱物을 嫌氣性消化시켜 그 탈리액을 炭素源으로 사용하였다. 炭素源의 공급없이 운전되는 A₂/O 工法과 嫌氣性消化槽를 설치한 A₂/O 工法의 두가지 Process에서 內部反送率을 변화시킴으로서 얻어지는 處理效果를 비교검토 하였다. 硏究結果, 두가지 Process에서 反送率이 燐 除去에 미치는 영향은 미미한 것으로 나타났으며, 窒素除去에 있어서는 反送率이 클수록 양호하게 나타났다. 嫌氣性消化槽 設置有·無에 따른 處理效率 比較에서는 流入原水 BOD의 감소에 따라 燐濃度가 적정비율을 초과하게 되는 경향이 있어서 설치하지 않은쪽의 處理效率이 더 우수한 결과를 나타냈다. The Anaerobic-Anoxic/Oxic process is one of the biological treatment methods to remove nitrogen and phosphate effectively which are nutritional elements for entrophication. Supernatant of primary sediment of Anaerobic digester is used as a carbon source instead of methanol supply in the usual A₂/O process. The efficiency of the following treatment processes are as follows; 1. Changing recycle rate in the usual A₂/O process without the stage of anaerobic disgester. 2. Changing recycle rate in the usual A₂/O process with the supernant supply of the anaerobic digester. In the result of comparison, changing recycle rate is almost no effect in the removal of phosphate, however the effect of removal in nitrogenous substance are remarkable, and the effect of anaerobic digester is not as effective as expected because the BOD removed in the digester partly, the rate of phosphates to the BOD exceed pertinent range.

알루미늄 연속주조 용탕의 탈 가스 일체화 장치 개발

이용중,김태원,김기대,류재엽,이형우 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

It is necessary for managing a perfect process for degasing aluminum molten metal according to the increase of a grade of aluminum and its alloy products. There are some methods that have been used to manage a degasing process in recent years, such as an injection method that uses aluminum molten metal powder and chemicals supplier and input method that supplies argon and nitrogen, or chlorine gas by using a gas blow-tube. However, these methods show some problems, and it shows that it is a difficult process to handle, pollution due to the producing a lot of toxic gases like chlorine and fluoride gas, irregular effects, and lowering work efficiency due to the excessive processing time. The problems that are the most fatal are the producing a lot of sludge due to the reaction of aluminum molten metal with chemicals, loss of metals, and decreasing the life of refractory materials. In order to solve these problems, this paper develops a technology that is related to aluminum continuous casting molten metal and monolithic degasing apparatus. A degasing apparatus developed in this study improved the existing methods and prevented environmental pollution with smokeless, odorless, and harmlessness by using a new method that applies argon and nitrogen gas in which the methods used in the West and Japan are eliminated. The developed method can significantly reduce product faults that are caused by the production of gas and oxidation because it uses a preprocessed molten metal with chemicals. In addition, the amount of the produced sludge can also be reduced by 60~80% maximum compared with the existing methods. Then, it makes it possible to minimize the loss of metals. Moreover, the molten metal processing and settling time is also shortened by comparing it with the existing methods that are applied by using chemicals. In addition, it does much to improve the workers' health, safety and environment because there is no pollution. The improvement of productivity and prevention effects of disaster from the results of the development can be summarized as follows. It will contribute to the process rationalization because it does not have any unnecessary processes that the molten metal will be moved to an agitator by using a ladle and returned to process for degasing like the existing process due to the monolithic configuration. There are no floating impurities due to the oxidation caused by the contact with the air as same as the existing process. In addition, it can protect the blending of precipitation impurities. Because it has a monolithic configuration, it can avoid the use of additional energy to compensate the temperature decreasing about 60? that is caused by the moving of molten metal. It is not necessary to invest an extra facilities in order to discharge the gas generated from a degasing process by using an agitator. The working environment can be improved by the hospitable air in the factory because the molten metal is almost not exposed in the interior of the area.

농장 사육되는 타조에서 나타난 다리 기형의 증례 보고

최종윤,손화영,전무형,조성환,김혜성,류시윤,박배근,이영원,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Two farmed young ostrich chicks were applied to pathology laboratory at Chungnam National University. Grossly, one ostrich showed rotated tibiotarsus, the other showed rolled toes. Case 1 ostrich was hypertrophy of fibualis longus and tibial cranialis tendon and gastrocnemius tendon in crus, inflammation of flexor digitorum longus. In histopathological views, myocytes of fiburalis longus were showed hypertrophy and hyperplasia. It is not found any specific feature in X-ray of the case 2 ostrich. Limb deformities were detected frequently in farmed ostrich chicks in 2- to 3 weeks old. Limb deformities were an important cause of mortality in farmed ostrich chicks during the first 10weeks after hatch. Cause of limb deformities were considered a nutrition problem.

돼지생식기호흡기 증후군(PRRS) 바이러스 감염시킨 돼지 기관의 병리학적 관찰

조성환,김현수,윤원기,전무형,류시윤,박배근,손화영,김무강 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The porcine reproductive and respiratory syndrome virus was inoculated into swine tracheal organ culture. The ballooning degeneration of the tracheal ciliated epithelium and a decrease of ciliary activity from the tracheal organs infected with PRRS virus were observed one day post-infection(PI). Tracheal epithelium showed degeneration and focal necrosis 2 days PI. Epithelial necrosis and loss of cilia and epithelial cells became more severe 3 and 4 days PI. An entire loss of cilia and epithelial cells were recognized 4 days PI, however, control tracheal organs were normal during the period of the experiment except a little decrease of ciliary activities with time..

Anthocyanin Contents Enhancement with Gamma Irradiated Mutagenesis in Blackberry (Rubus fructicosus)

Ryu, Hyung Won,Cho, Byoung Ok,Ryu, Jaihyunk,Jin, Chang Hyun,Kim, Jin-Baek,Kang, Si Yong,Han, Ah-Reum NATURAL PRODUCT COMMUNICATIONS 2017 Natural product communications Vol.12 No.9

<P>Effects of radiation processing on the endogenous phytochemical production in blackberry (Rubus fructicosus L.) were investigated through a metabolites analysis, using high-performance liquid chromatography with a diode array detector and a triple quadrupole mass spectrometer (HPLC-DAD-ESI-MS/MS). Four mutant cultivar lines (B, C, D, and E) were developed through the treatment of various doses of gamma irradiation (80, 60, 40, and 20 Gy) on the control blackberry cultivar (V3). These mutant cultivar lines were selected according to their different morphological variations in leaf shape and branch color. In the present study, we performed quantitative analyses of the four standard compounds in blackberry mutant cultivars: cyanidin-3-O-glucoside, cyanidin-3-Oxyloside, cyanidin-3-O-malonylglucoside, and cyanidin-3-O-dioxalylglucoside. The HPLC fingerprints displayed different profiles for six blackberry cultivar lines, demonstrating that the accumulation of total anthocyanins was affected by the gamma irradiation. The contents of cyanidin-3-O-glucoside and cyanidin-3-O-xyloside were high in the gamma-irradiated mutant cultivars of blackberries, compared to those of the control cultivar (V3) and the cross breeding cultivar (R. fructicosus x R. parvifolius; CB). Cyanidin-3-O-malonylglucoside and cyanidin-3-O-dioxalylglucoside were also analyzed for their quantification in six cultivar lines, as metabolites contributing to the discrimination of six cultivar lines. Among gamma irradiated mutant blackberry cultivars, the highest dose level of gamma irradiation (80 Gy) resulted in the highest cyanidin-3-O-glucoside content enhancement. Therefore, these results can be useful to determine the optimized gamma irradiated value at which anthocyanins reaches an abundant level in Rubus species.</P>

Inhibition Effects of Mangosenone F from Garcinia mangostana on Melanin Formation in B16F10 Cells

Ryu, Hyung Won,Jeong, Seong Hun,Curtis-Long, Marcus J.,Jung, Sunin,Lee, Ji Won,Woo, Hyun Sim,Cho, Jung Keun,Park, Ki Hun American Chemical Society 2012 Journal of agricultural and food chemistry Vol.60 No.34

<P>Melanogenesis can be controlled by tyrosinase inhibition or by blocking the maturation processes of tyrosinase and its related proteins. Mangostenone F was isolated from the seedcases of Garcinia mangostana. Mangostenone F was shown to be inactive against tyrosinase (IC<SUB>50</SUB> > 200 μM) but was a potent α-glucosidase inhibitor in vitro (IC<SUB>50</SUB> = 21.0 μM). Mangostenone F was found to inhibit production of melanin in the mouse melanoma cell line B16F10. Importantly, unlike most glycosidase inhibitors, mangostenone F displayed very low cytotoxicity (EC<SUB>50</SUB> > 200 μM). The Western blot for expression levels of proteins involved in melanogenesis showed that mangostenone F down-regulated tyrosinase and TRP-2 expression. Treating B16F10 cells with mangostenone F significantly increased the susceptibility of tyrosinase to endoglycosidase H digestion, indicating that tyrosinase was unable to mature fully and pass to the <I>trans</I>-golgi apparatus. Consistent with these data, in lysate assays, mangostenone F was shown to be a better inhibitor of α-glucosidases than deoxynojirimycin, a representative glycosidase inhibitor.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2012/jafcau.2012.60.issue-34/jf3015987/production/images/medium/jf-2012-015987_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf3015987'>ACS Electronic Supporting Info</A></P>

Association of GATA5 methylation with clinocopathological characteristics in non-muscle invasive bladder cancer

Hyung-Yoon Yoon,Sung Pil Seo,Sang Keun Lee,Ho Won Kang,Won Tae Kim,Dong Hee Ryu,Seok-Joong Yun,Sang-Cheol Lee,Wun-Jae Kim,Yong-June Kim 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.4

DNA methylation is the most common and well-characterized epigenetic change in human cancer. Recently, the association between GATA-binding protein 5 (GATA5) methylation and carcinogenesis of various types of tumors was investigated. The aim of the present study was to evaluate the effect of GATA5 methylation status on clinicopathological features and prognosis in primary non-muscle invasive bladder cancer (NMIBC) patients with a long-term follow-up period. The GATA5 methylation status was determined for 171 human bladder specimens (eight normal controls [NCs] and 163 primary NMIBC patients) using quantitative pyrosequencing analysis. The primary NMIBC tissues were obtained from patients who underwent transurethral resection (TUR) for histologically diagnosed transitional cell carcinomas between 1995 and 2012 at Chungbuk National University Hospital. GATA5 methylation was significantly higher in NMIBC patients than in NCs and was significantly associated with higher grade and more advanced stage of cancer. Kaplan-Meier estimates showed significant differences in tumor recurrence and progression according to GATA5 methylation status (each p<0.05). Our results show that increased methylation of GATA5 was significantly associated with not only aggressive characteristics but also poor prognosis in primary NMIBC patients. Alteration of GATA5 methylation might be used as a biomarker for prognosis of NMIBC patients. However, prospective and functional investigations are necessary to clarify the role of GATA5 methylation in future clinical management of patients with NMIBC.

Metabolic Profile Analysis of Glycyrrhizae Radix Distributed in Korean Market

Hyung Won Ryu(류형원),Jeong Hoon Lee(이정훈),Yun Ji Lee(이윤지),Jin Tae Jeong(정진태),Chun Geon Park(박춘근),Yong Il Kim(김용일),Jae Ki Chang(장재기),Sei Ryang Oh(오세량) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.1

Potential Anti-inflammatory Effects of the Fruits of <i>Paulownia tomentosa</i>

Ryu, Hyung Won,Park, Yhun Jung,Lee, Su Ui,Lee, Seoghyun,Yuk, Heung Joo,Seo, Kyeong-Hwa,Kim, Yeah-Un,Hwang, Bang Yeon,Oh, Sei-Ryang American Chemical Society and American Society of 2017 Journal of natural products Vol.80 No.10

<P>As part of an ongoing search for new natural products from medicinal plants to treat respiratory disease, six new compounds, a dihydroflavonol (1) and five C-geranylated flavanones (3, 6, 8, 13, and 14), and 13 known compounds were isolated from mature fruits of Paulownia tomentosa. The structures of the new compounds were determined via interpretation of their spectroscopic data (1D and 2D NMR, UV, IR, ECD, and MS). In biological activity assays with human alveolar basal epithelial cells, the expression of TNF-alpha-induced proinflammatory cytokines (IL-8 and IL-6) was reduced significantly by the EtOAc fraction of a P. tomentosa extract as well as by the new compounds isolated from this fraction. Furthermore, the majority of the isolates (1-19 except 5-7) were found to inhibit human neutrophil elastase (HNE) activity, with IC50 values ranging from 2.4 +/- 1.0 to 74.7 +/- 8.5 mu M. In kinetic enzymatic assays with the HNE substrate MeOSuc-AAPV-pNA, compound 17 exhibited the highest inhibitory activity (K-i = 3.2 mu M) via noncompetitive inhibition. These findings suggest that the flavanone constituents of P. tomentosa fruits may be valuable for the development of new drug candidates to treat airway inflammation.</P>

Inhibition of Xanthine Oxidase by Phenolic Phytochemicals from Broussonetia papyrifera

Ryu, Hyung Won,Lee, Ji Hye,Kang, Jae Eun,Jin, Young Min,Park, Ki Hun 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5

The roots of Broussonetia papyrifera were extracted into four different polar solvents: chloroform, 50% ethanol in water, ethanol, and water. The ethanol extract showed the most potent inhibition (72.3% at 20 g/mL) against xanthine oxidase (XOD). Chromatography of EE yielded nine phenolic phytochemicals, which were confirmed as broussochalcone A (1), broussochalcone B (2), 3,4-dihydroxyisolonchocarpin (3), 4-hydroxyisolonchocarpin (4), 3-'(3-methylbut-2-enyl)-3',4',7-trihydroxyflavane (5), kazinol A (6), kazinol B (7), kazinol E (8), and broussoflavan A (9). All isolated compounds (19) possessed potent antioxidant activities against 2,2-diphenyl-l-picrylhydrazyl and 2,2'-azino-bis-ethylbenzthiazoline-6-sulfonic acid (ABTS) radicals with $IC_{50}$ values ranging from 5.8 to 252.8M. Although most compounds exhibited potent inhibition with $IC_{50}$ values ranging 0.6-164 M against XOD, compounds 1 and 3 were found to be the principal contributors to the XOD inhibition in ethanol extract. The analysis of $K_I$ and $K_{IS}$ values proved that the two most promising compounds (1 and 3), present at high concentrations in the root barks as analyzed by using high-performance liquid chromatography analysis, were reversible mixed type I inhibitors.