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Recent activity of mouse metabolic phenotyping service at Korea Mouse Phenotyping Center (KMPC)
Hye Sun Go,Ji Min Choi,Seul Gi Yoon,Su In Jang,Soo Jin Son,Da In On,Hyun A Noh,Mi Young Kim,Il Yong Kim,Je Kyung Seong 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
To understand the function of individual genes is also a key information in developing new treatment techniques. Even though a couple of genetically engineered mouse (GEM) models has been generated, still precising determination of mouse phenotype is not easy. Precising mouse phenotyping is one of the effective way leading to discovery gene function. International Mouse Phenotyping Consortium (IMPC) has established mega database of mouse phenotyping data from different institutes across the world based on generalized platform. However more precised mouse phenotyping is still needed. In order to meet the need for more detailed phenoyping in mouse, Korea Mouse Phenotyping Center (KMPC), nation-wide program for mouse production and phenotyping in Korea has been establishing several pipelines for disease-specific mouse phenotyping to support the mouse research. Here we introduce mouse metabolic and exercise phenotyping services, as well as the other services of mouse research such as providing genetically engineered mouse information, producing selling genetically engineered mice, and managing resource quality so that researchers can easily utilize the research infrastructure. Metabolic characterization in mouse is one of key factors for understanding the pathogenesis of obesity, type 2 diabetes and insulin resistance. KMPC has been providing mouse metabolic phenotyping including high fat diet, exercise and cold challenges. Multiple parameter including energy expenditure (EE), O2/CO2 consumption (RER), heat generation and activity has been provided with histology service and body composition. Also temperature can be measured during metabolic chamber with telemetric system. Here we summarized mouse metabolic phenotyping services at KMPC.
Jin, Hyun-Seok,Kim, Jeonghyun,Lee, Soo-Jin,Kim, Kyunga,Go, Min Jin,Lee, Jong-Young,Lee, Hye-Ja,Song, Jihyun,Jeon, Byeong Tak,Roh, Gu Seob,Kim, Sung-Jun,Kim, Bo-Young,Hong, Kyung-Won,Yoo, Young-Hyun,Oh Elsevier 2014 Molecular and cellular endocrinology Vol.382 No.1
<P><B>Abstract</B></P> <P>Several association studies have implicated the <I>PARK2</I> gene that encodes parkin – the key molecule orchestrating the mitochondrial quality control system – as a candidate susceptibility gene for diabetes. A total of 7551 unrelated Korean KARE cohort subjects were analyzed to investigate the association between the <I>PARK2</I> single nucleotide polymorphism (SNP) and quantitative glycemic traits. Two SNPs, rs10455889 and rs9365294, were significantly associated with fasting plasma glucose level (<I>p</I> =∼1.2×10<SUP>−4</SUP>) and insulin secretion indices (<I>p</I> =∼7.4×10<SUP>−5</SUP>) in male KARE subjects. Parkin was expressed predominantly in the rat pancreatic islets. Downregulation of the <I>Park2</I> gene in rat INS-1 β-cells resulted in a significant decrease in the glucose-stimulated insulin secretion, intracellular insulin gene expression, and intracellular ATP level. The <I>Park2</I>-depleted β-cells also exhibited increased mitochondrial fragmentation and ROS production and decreased mitochondrial membrane potential. Both population-based statistical evaluation and experimental evidence demonstrated a fundamental role of the <I>PARK2</I> gene in the maintenance of β-cell function.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Two <I>PARK2</I> SNPs are associated with fasting blood glucose and insulin secretion. </LI> <LI> The <I>Park2</I>-depleted rat β-cells exhibit decreased insulin production and secretion. </LI> <LI> Knockdown of <I>Park2</I> gene by RNAi causes mitochondrial alterations in rat INS-1 β-cells. </LI> <LI> <I>PARK2</I> gene plays an important role in the maintenance of pancreatic β-cell function. </LI> </UL> </P>
Application of Cidea reporter mice, brown adipogenesis in iWAT
Jin Kyung Kim,Hye Sun Go,Sol Pin Kim,Il Yong Kim,Yun Hee Lee,Seung Hyun Oh,Ho Lee,Je Kyung Seong 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Obesity is one of the most serious diseases in modern society as energy intake and energy expenditure make imbalance. As treating the obesity becomes global issue, a number of treatments have been proposed and exercise is getting attention as a one way. It is well known that exercise has numerous benefits that affect whole body metabolism. Especially it can induce ‘browning’ of white adipocyte in adipose tissue. Cidea (Cell Death Inducing DFFA Like Effector A) is one of the disease associated genes including metabolic disorder and adipogenesis. It has been widely used for brown adipogenesis marker. We developed Cidea-dual reporter mouse (Cidea-P2A-Luc2-T2A-tdTomato, Luciferase/tdTomato) for applying in vivo tracing and measuring brown adipogenesis. Cidea-dual reporter mouse performed voluntary wheel running for 8 weeks. CL was injected intraperitoneally (1 mg/kg of body weight/day) for 3 days to Cidea-reporter mouse for inducing brown adipogenesis. Brown adipogenesis in Cidea-dual reporter mouse was measured not only by optical imaging system but also by histology. As a result of exercise, browning occurred depots in white adipose tissue become luminescent. We confirmed that exercise and CL can induce brown adipogenesis with in vivo reporter system. Cidea-dual reporter mouse will be widely used for detecting brown adipogenesis.
Dexamethsone Cotreatment Antoginizes FK506-induced Antitumor Effects in Hepatocarcinoma Cell
Hye Min Park,Sei Jin Lee,Hyeon Kyu Go,Ypung Ran Park,A Reum Mun,Ra Mi Park,Gi Beum Kim,Seong Jong Kim,Sung Zoo Kim,Chul Un Hong,Jin Shang Kim,Hyung Sub Kang,Shang Jin Kim 대한수의학회 2011 대한수의학회 학술대회발표집 Vol.2011 No.-