KI-180과 KI-188 칼슘조성물이 어린쥐의 성장발육에 미치는 영향

박승만(Seung-Man Park),성기승(Ki-Seung Seong),이종석(Jong-Seok Lee),이옥환(Ok-Hwan Lee),한찬규(Chan-Kyu Han) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3

본 연구에서는 KI-188 칼슘과 KI-180 조성물이 성장발육에 미치는 효과를 조사하였다. 시험기간중 KI-188 칼슘과 KI-180 식이군이 대조군 보다 성장률과 평균 식이섭취량은 증가하였다. 체장은 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 평균 3.55 mm 더 길었고, 등뼈길이는 KI-180 식이군이 대조군 보다 통계적인 차이는 없었지만 0.5 mm 더 길었다. 대퇴골 무게는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 무거웠으며, 대퇴골길이는 대조군에 비해 각각 평균 0.89, 1.09 mm 더 길었다. 혈당, 총콜레스테롤 및 중성지방 농도는 차이가 없었고, 칼슘농도는 KI-188 칼슘과 KI-180 식이군 보다 대조군이 유의하게 높았다. 백혈구와 혈소판수는 차이가 없었고, 적혈구, 혈색소 및 헤마토크릿치는 대조군이 KI-188 칼슘과 KI-180 식이군 보다 통계적으로 높았다. ALP활성은 KI-180 식이군이 대조군 보다 통계적으로 높았고, osteocalcin농도는 KI-188 칼슘과 KI-180 식이군이 대조군에 비해 통계적으로 유의하게 높았다. 혈청 testosterone 농도는 KI-188 칼슘과 KI-180 식이군과 대조군 간에 차이가 없었다. IGF-1과 IGFBP-3 농도는 KI-180 조성물이 대조군 보다 각각 20%, 11% 정도 유의하게 더 높았다. 이상의 결과를 검토할 때 KI-188 칼슘과 KI-180 조성물은 어린쥐의 성장과 골격의 발달을 촉진시키고, 성장호르몬의 분비능을 양적으로 유도하여 전반적인 성장발육에 유효한 작용이 있는 것으로 사료된다. Herbs have active components that promote the growth rate of both animals and human. The KI-180 and KI-188 calcium food formulae contain Acanthopanacis cortex, Bombysis corpus and hoelen, seaweed calcium, chlorella extract, spirulina, colostrum powder, and other natural and functional components. We evaluated the growth-promoting effects of these formulae by analyzing the weight, femur and backbone, alkaline phosphatase, osteocalcin, testosterone, insulin-like growth factor (IGF)-1, and IGF binding protein-3 (IGFBP-3) of growing rats. Growing rats administered with KI-180 and KI-188 calcium showed the increase of body weight, body length, and femur weight and length of growing rats. In addition, administration of KI-180 and KI-188 calcium increased the alkaline phosphatase activity, the levels of osteocalcin and the growth hormones IGF-1 and IGFBP-3 of growing rats. The impact of KI-180 and KI-188 calcium on the physical development of growing rats suggests that the incorporation of these food formulae in the diets of growing children may promote the physical development.

비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.

강소기업의 지속성장을 위한 경영권 승계 철학

박기찬(Ki-Chan Park),이윤(Yune Lee),이한구(Han-Gu Lee) 피터드러커 소사이어티 2014 창조와 혁신 Vol.7 No.2

본 사례연구는 창업으로 성공한 창업가(entrepreneur)의 경영권 승계에 대한 철학을 그의 사업 역정을 통하여 살펴보는 데 목적이 있다. 흔히 경영자(manager)의 눈에는 돈이 안 되는 문제부터 보이지만, 사업가(businessman)의 눈에는 돈이 되는 사업부터 보인다고 한다. 과연 우리나라에 창업 사업가로서 기업가 정신(entrepreneurship)을 잃지 않으면서 한편으로는 경영자로서 '創業보다 어렵다는 守成의 길'을 성공적으로 영위해 온 인물은 얼마나 되는가? 그리고 언젠가는 자신의 사업체를 누군가에게 물려줄 수밖에 없는 상황에서 경영권 승계는 과연 어떻게 이루어지고 있는가? 이에 대한 논의를 위해 본 사례는 코텍 이한구 회장과의 인터뷰를 바탕으로 사업가이자 최고경영자로서 사업과 인생에 대한 관점 및 그의 경영권 승계에 대한 철학을 분석함으로써 강소기업으로 성공한 창업가(entrepreneur)는 물론 대기업의 경영권 승계 관행에 대한 새로운 시사점을 던지고자 한다. The purpose of this case study is to analyze the philosophy of an entrepreneur (CEO and Chairman of Kortek) being appeared in the process of management succession. The 27 years history of a start-up firm introduced in this analysis maneuvered its challenging business based on Chairman Lee's business insights and philosophy since its foundation in 1987. Kortek, as a hidden champion, is the No. 1 in the field of Gaming Video Display Monitor delivering more than 70% of Video Slut Machines in the U.S. market. Compared to the ordinary managers who are in seeking of dysfunctions of daily management and operations, the founder and current CEO and Chairman Han-Gu Lee pursued to catch out the money-making businesses with his talented entrepreneurship. Despite of his unprecedent success as an industrial display monitor maker, recently he has been deliberately considered about the management succession scenarios for the vision of his cherished Kortek to keep as a sustainable company. In this case study, we analyzed the 3 scenarios being evaluated during more than 2 years by Chairman Lee. And as the result and performances after his decision to accept M&A with IDIS Holdings show a sustainable growth, we could extract strong implications not only for the Korean CEOs of hidden champions who have experiences as start-up founder but also other major companies being faced with such an agony in management succession.

Interrelation of Cyclin D1, Cyclin E, and p27Kip1 Expression on Tissue Arrays of Breast Cancer

Sehwan Han,Young-Duck Kim,Hong-Yong Kim,Hong-Joo Kim,Kyeongmee Park,Byung-Noe Bae,Suk Yong Ryu,Ki Hwan Kim 대한암학회 2002 Cancer Research and Treatment Vol.34 No.5

Purpose: To evaluate the clinical impact of the alteredexpression of cell cycle regulators in stage I and II breastcancers.Materials and Methods: The interaction between cyclinD1/E and p27Kip1 expressions were analyzed usingtissue microarray (TMA) technology in 133 breast cancers.Data from the immunohistochemical assays of 3molecules were merged, and analyzed, with a Ki67labeling index of the same tumors.Results: Cyclin D1 was expressed in 72 breast carcinomas(54.1%) and cyclin E in 60 (45.1%) out of the 133breast carcinomas. Expressions of cyclin D1 and cyclinE were inversely related to each other, and significantlyassociated with the estrogen receptor (ER) expressionand differentiation of the breast carcinoma. The expressionof cyclin E was significantly decreased in tumorsexpressing cyclin D1 (p=0.022). There was a trend forcyclin D1 expression to increase in tumors expressingp27Kip1 (p=0.053), but the expression of cyclin E didnot correlate with p27Kip1 expression. The Ki67 labelingindex was markedly increased in tumors expressing cyclinE, whereas it was significantly decreased in the cyclin D1or p27Kip1 expressing-tumors. From survival analysis,cyclin E expression was the only significant variable forthe prediction of poor survival.Conclusion: The abnormal expressions of cell cycleregulatory molecules are prevalent, and interrelated witheach other in breast cancer. Integration of TMA technologyallowed a high-throughput analysis for correlating molecularthe in situ findings, with the clinico-pathologicinformation. Among the three molecules studied, thecyclin E had a prognostic implication for stage I and IIbreast cancer. (Cancer Res Treat. 2002;34:388-393)

강소기업의 지속성장을 위한 경영권 승계 철학

박기찬(Ki-Chan Park),이윤(Yune Lee),이한구(Han-Gu Lee) 피터드러커 소사이어티 2014 창조와 혁신 Vol.7 No.1

본 사례연구는 창업으로 성공한 창업가(entrepreneur)의 경영권 승계에 대한 철학을 그의 사업 역정을 통하여 살펴보는 데 목적이 있다. 흔히 경영자(manager)의 눈에는 돈이 안 되는 문제부터 보이지만, 사업가(businessman)의 눈에는 돈이 되는 사업부터 보인다고 한다. 과연 우리나라에 창업 사업가로서 기업가 정신(entrepreneurship)을 잃지 않으면서 한편으로는 경영자로서 '創業보다 어렵다는 守成의 길'을 성공적으로 영위해 온 인물은 얼마나 되는가? 그리고 언젠가는 자신의 사업체를 누군가에게 물려줄 수밖에 없는 상황에서 경영권 승계는 과연 어떻게 이루어지고 있는가? 이에 대한 논의를 위해 본 사례는 코텍 이한구 회장과의 인터뷰를 바탕으로 사업가이자 최고경영자로서 사업과 인생에 대한 관점 및 그의 경영권 승계에 대한 철학을 분석함으로써 강소기업으로 성공한 창업가(entrepreneur)는 물론 대기업의 경영권 승계 관행에 대한 새로운 시사점을 던지고자 한다. The purpose of this case study is to analyze the philosophy of an entrepreneur (CEO and Chairman of Kortek) being appeared in the process of management succession. The 27 years history of a start-up firm introduced in this analysis maneuvered its challenging business based on Chairman Lee's business insights and philosophy since its foundation in 1987. Kortek, as a hidden champion, is the No. 1 in the field of Gaming Video Display Monitor delivering more than 70% of Video Slut Machines in the U.S. market. Compared to the ordinary managers who are in seeking of dysfunctions of daily management and operations, the founder and current CEO and Chairman Han-Gu Lee pursued to catch out the money-making businesses with his talented entrepreneurship. Despite of his unprecedent success as an industrial display monitor maker, recently he has been deliberately considered about the management succession scenarios for the vision of his cherished Kortek to keep as a sustainable company. In this case study, we analyzed the 3 scenarios being evaluated during more than 2 years by Chairman Lee. And as the result and performances after his decision to accept M&A with IDIS Holdings show a sustainable growth, we could extract strong implications not only for the Korean CEOs of hidden champions who have experiences as start-up founder but also other major companies being faced with such an agony in management succession.

Benzyl Isothiocyanate Inhibits Prostate Cancer Development in the Transgenic Adenocarcinoma Mouse Prostate (TRAMP) Model, Which Is Associated with the Induction of Cell Cycle G1 Arrest

Cho, Han Jin,Lim, Do Young,Kwon, Gyoo Taik,Kim, Ji Hee,Huang, Zunnan,Song, Hyerim,Oh, Yoon Sin,Kang, Young-Hee,Lee, Ki Won,Dong, Zigang,Park, Jung Han Yoon MDPI 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.2

<P>Benzyl isothiocyanate (BITC) is a hydrolysis product of glucotropaeolin, a compound found in cruciferous vegetables, and has been shown to have anti-tumor properties. In the present study, we investigated whether BITC inhibits the development of prostate cancer in the transgenic adenocarcinoma mouse prostate (TRAMP) mice. Five-week old, male TRAMP mice and their nontransgenic littermates were gavage-fed with 0, 5, or 10 mg/kg of BITC every day for 19 weeks. The weight of the genitourinary tract increased markedly in TRAMP mice and this increase was suppressed significantly by BITC feeding. H and E staining of the dorsolateral lobes of the prostate demonstrated that well-differentiated carcinoma (WDC) was a predominant feature in the TRAMP mice. The number of lobes with WDC was reduced by BITC feeding while that of lobes with prostatic intraepithelial neoplasia was increased. BITC feeding reduced the number of cells expressing Ki67 (a proliferation marker), cyclin A, cyclin D1, and cyclin-dependent kinase (CDK)2 in the prostatic tissue. <I>In vitro</I> cell culture results revealed that BITC decreased DNA synthesis, as well as CDK2 and CDK4 activity in TRAMP-C2 mouse prostate cancer cells. These results indicate that inhibition of cell cycle progression contributes to the inhibition of prostate cancer development in TRAMP mice treated with BITC.</P>

Expression Profiles of Retinoblastoma Protein and p27Kip1 According to HER2 Amplification Status in Primary Breast Cancer

신재호,Sung-Jin Park,Hyun-Jin Cho,Geumhee Gwak,Byung-Noe Bae,Ki Whan Kim,Hong-Yong Kim2,Kyeongmee Park,Sehwan Han 한국유방암학회 2009 Journal of breast cancer Vol.12 No.1

Purpose: Human epidermal growth factor receptor-2 (HER-2)/neu amplification affects the cell proliferation through the modulation of multiple G1 cell cycle regulators in breast tumor cells. We performed this study to investigate whether retinoblastoma protein (pRB) and p27Kip1 were differently expressed according to the HER2 amplification status in human breast cancer. Methods: HER2 amplification was assayed by fluorescence in situ hybridization and the expression of cell cycle regulators were assayed by immunohistochemistry on 153 consecutive invasive breast cancers. The proliferative activity of breast cancer was analyzed according to the HER2 amplification and cell cycle protein expression status. Results: HER2 amplification was observed in 39 (25.5%) of 153 breast cancers. In the HER2 amplified breast cancers, the pRB expression was significantly increased (p=0.011) whereas there was no significant relationship between HER2 amplification and p27Kip1 expression. There was an inverse correlation between pRB expression and Ki-67 labeling index in the HER2 amplified breast cancers (p=0.036). In contrast, Ki67 labeling index was significantly decreased as p27Kip1 expression increased in HER2 non-amplified breast cancers (p=0.028). In HER2 non-amplified breast cancers, we could not observe any association between the pRB expression and Ki67 labeling index. Conclusion: The proliferation of the breast cancers was associated with pRB expression in HER2 amplified tumors whereas it was associated with p27Kip1 expression in HER2 non-amplified tumors. The results of the current study indicate that the cell proliferative activity of the breast cancer is under different growth signal pathways according to HER2 amplification status.

운동선수의 임장불안에 대한 연구

홍관이,이광재,김두경,부기원,노성규,한상준,박기동,오수일,문병용,이철규,박장평,엄기진,박남환 江原大學校附設 體育科學硏究所 1984 江原大學校附設體育科學硏究所論文集 Vol.- No.9

This study is designed to grasp the elements and situations of stage fright in order to propose a part of ways to cope with it. We make 511 top-class high school players, male and female, in the central districts (Seoul, Inchon, Kyungki, Chungbuk, and Kangweon Province) on object of questionnaire, and get the following results. Through players generally have all-round characters they show weakness in the superiority and emotional stability while showing strength in the sonformity. This fact comes to be related to the elements which cause stage fright. Physiological changes also have many effects on the symptom of stage fright. Especially, stage fright increases when players show sensitive reaction to the self-consciousness of final consequence which comes from the tension or uneasiness of sympathetic nerve, Thus, it turns out that players make use of reducing psychological burden by physical exercise or adjusting the level of demanded result in order to release the stage fright.

학교체육 활성화 방안에 대한 연구(Ⅰ) : 강원대학교 교양체육 운영방법의 개선을 중심으로

이광재,문병용,노성규,박기동,홍관이,한상준,유옥재,엄기진,정청자,오수일,김윤래,박장평,부기원 江原大學校附設 體育科學硏究所 1986 江原大學校附設體育科學硏究所論文集 Vol.- No.11

Try to find a scheme for activation to College Physical Education, this Report informs the various kinds of Survey and process those were projected for improvement of management methods on Cultural Physical Education in Kang Weon National University. From experimentally carried out "Sports events Choosing System" not as usual be inforced "Total practical Skill System", Following Positive effects and sujestions were Presented; 1. "Sports events choosing system" gave birth to more students' interesting and more voluntary participation to the sports events they chose, and sports skill and instruction level was elevated due to charge of expert according to each sports events. This system would be managed continuously hereafter for it related to life sports. 2. This System needs to closed cooperations between College and Community, for the facilities of community are utilized by college. 3. To complete the plan for activation of college Physical Education, additionally, the successional study ; namely "autonomous extracurricular sports activity" should be excuted hereafter.

Pediatric infratentorial ependymoma: prognostic significance of anaplastic histology.

Phi, Ji Hoon,Wang, Kyu-Chang,Park, Sung-Hye,Kim, Il Han,Kim, In-One,Park, Kyung Duk,Ahn, Hyo Seop,Lee, Ji Yeoun,Son, Young-Je,Kim, Seung-Ki M. Nijhoff ; Kluwer Academic Publishers 2012 Journal of neuro-oncology Vol.106 No.3

<P>Pediatric infratentorial ependymomas are difficult to cure. Despite the availability of advanced therapeutic modalities for brain tumors, total surgical resection remains the most important prognostic factor. Recently, histological grade emerged as an independent prognostic factor for intracranial ependymoma. We retrospectively reviewed the treatment outcome of 33 pediatric patients with infratentorial ependymoma. Progression-free survival (PFS) and overall survival (OS) rates were calculated and relevant prognostic factors were analyzed. Fourteen patients (42%) were under the age of 3 at diagnosis. Gross total resection was achieved in 16 patients (49%). Anaplastic histology was found in 13 patients (39%). Adjuvant therapies were delayed until progression in 12 patients (36%). Actuarial PFS rates were 64% in the first year and 29% in the fifth year. Actuarial OS rates were 91% in the first year and 71% in the fifth year. On univariate analysis, brainstem invasion (P = 0.047), anaplastic histology (P = 0.004), higher mitotic count (P = 0.001), and higher Ki-67 index (P = 0.004) were significantly related to a shorter PFS. Gross total resection (P = 0.029) and a greater age at diagnosis (P = 0.033) were significantly related to a longer PFS. On multivariate analysis, anaplastic histology alone was significantly related to a shorter PFS (P = 0.023). Gross total resection (P = 0.039) was significantly related to a longer overall survival (OS) on multivariate analysis. Anaplastic histology and gross total resection were the most important clinical factors affecting PFS and OS, respectively. Anaplastic histology, mitotic count, and Ki-67 index can be used as universal and easily available prognostic parameters in infratentorial ependymomas.</P>