이뇨제의 저항성과 내성 기전

오윤규,나기영,김근호,김소영,한진석 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

신손상 진단에 있어 현미경적 혈뇨의 의의

임정환,오봉렬,나국주,김현수,윤한덕,허 탁,조석주,민용일 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

The evaluation of patients with microscopic hematuria after blunt abdominal trauma has become controversial. We tested the hypothesis that renal contusion can be diagnosed clinically and that these patients do not require radiographic evaluation. To evaluate the association of microscopic hematuria without shock and with renal injury, we reviewed the medical records of 252 patients following blunt abdominal trauma. Microscopic hematuria without shock was noted in 58 of 252 patients. Of the 58 patients 52 had renal contusion, 4 had renal laceration, 1 had a renal rupture and 1 had renal pedicle injury. All of the patients with renal contusions experienced no complications from nonoperative management. But, avoiding a radiopraphic evaluation in patients with blunt renal trauma plus microscopic hematuria and no shock would miss a few cases of severe renal injury.

남성 알코올 의존 환자의 삶의 질과 정신-사회-영적 특성과의 관계

최삼욱,나란희,김한오,최성빈,최영숙 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.5

Objectives : This study was designed to evaluate the relationship between quality of life (QOL) and psycho-socio-spiritual characteristics in male patients with alcohol dependence. Methods : The sample consisted of 109 men with alcohol dependence defined by DSM-IV criteria. We assessed QOL by the WHO QOL assessment instrument-BREF (WHOQOL) and SmithKline Beecham QOL (SBQOL). Sociodemographic and alcohol related data were collected, and 7 questionnaires were administered : MAST, BDI, STAI, Drinker Inventory of Consequences (DrInC), Scale of Social Support (SSS), Religious Beliefs and Behaviors (RBB) and Spiritual Well-being Scale (SWBS). The correlations between each QOL score and other variables were examined, and stepwise multiple regression analysis was performed. Results : The WHOQOL score positively correlated with education level, SSS (support) and RBB and negatively correlated with MAST, DrInC, BDI, STAI (trait) and SSS (conflict) scores. In stepwise regression analysis, the scores on the STAl and BDI contributed to the score on the WHOOOL. The SBQOL score correlated with the income level, and negatively correlated with BDI and STAI score. STAI score was a weak predictor of SBQOL score. Conclusion : The significant predictors of QOL in patients with alcohol dependence were psychological factors such as anxiety and depression.

장기간 이뇨제 투여에 따른 요 산성화 능력과 집합관 H^(+)-ATPase 발현의 변화

김근호,나기영,한진석,오윤규,이정상,주권욱,엄재호 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

목 적 : 저자들은 임상에서 흔히 사용하면서 대사성 알칼리증을 유발할 수 있는 furosemide와 hydrochlorothiazide를 장기간 투여할 때 발생하는 요 산성화능의 변화를 관찰하고, 이에 동반하여 근위세관 및 헨리고리관의 NHE3와 집합관의 H^(+)-ATPase 단백 발현에 변화가 있는지 조사하고자 하였다. 방 법 : Sprague-Dawley rat에서 수분과 전해질 용액을 자유롭게 섭취시키면서 furosemide12 mg/day/rat 혹은 hydrochlorothiazide 7.5 mg/day/rat을 각각 7일간 지속적 피하 주입시킨 후 신장에서 반정량적 immunoblot 분석을 시행하여 대조군과 비교하였다. 결 과 : Furosemide 투여 후 대조군과 실험군 사이에 체중, 혈청 알도스테론 및 크레아티닌청소율은 차이가 없었다. 요량 및 요 소디움 배설은 furosemide 투여 후 현저하게 증가하였다. 요 pH가 furosemide 투여 후 감소하였고, 요 암모늄 배설은 furosemide 투여 후 증가하였다. 반정량적 immunoblot 분석 결과 furosemide 투여 후 신 피질의 NHE3 발현에 유의한 변화는없었으나, 신 외수질의 NHE3 발현은 실험군(182±25%)에서 대조군(100±25%)에 비해 증가하였다(p<0.05). 또한, H^(+)-ATPase B1 subunit 발현은 furosemide 투여 후 신 피질(대조군100±13%, 실험군 178±11%, p<0.01)과 신 외수질(대조군 100±29%, 실험군 239±24%, p<0.05)에서 모두 증가하였다. Hydrochlorothiazide 투여 실험에서도 요량 및 요 소디움 배설의 증가와 요 pH의 감소를 확인하였다. 신 피질과 외수질의 NHE3 발현은 hydrochlorothiazide투여 후 변화 없었으나, H^(+)-ATPase B1 subunit 발현은 신 피질(대조군 100±24%, 실험군212±27%, p<0.05)과 신 외수질(대조군 100±13%, 실험군 194±13%, p<0.01)에서 모두 증가하였다. 결 론 : 장기간 furosemide 혹은 hydrochlorothiazide 투여에 의해 원위 요 산성화 능력의 항진을 확인하였고, 이는 집합관 H^(+)-ATPase 단백 발현이 증가하여 발생한 결과로 설명할 수 있을 것으로 생각한다. Purpose : Commonly used diuretics such as furosemide and hydrochlorothiazide may cause metabolic alkalosis by increasing proton secretion from distal nephron. We evaluated changes in urinary acidification and abundance of proton-secreting transporters in response to chronic subcutaneous infusion of diuretics. Methods : Osmotic minipumps were implanted into Sprague-Dawley rats to deliver 12 mg/day furoemide or hydrochlorothiazide 7.5 mg/day for 7 days. All animals were offered tap water and a solution containing 0.8% NaCl and 0.1% KCl as drinking fluid. Results : Compared with vehicle-infused controls, diuretic and natriuretic responses were evident from furosemide or hydrochlorothiazide infusion. However, there were no changes in body weight, serum aldosterone and creatinine clearance between diureticinfused( n=6) and control(n=6) rats. In both furosemide- infused and hydrochlorothiazide-infused rats, urine pH was significantly lowered compared with controls. Furosemide-infused rats showed significantly larger excretion of urinary ammonium. Semiquantitative immunoblotting was carried out from rat kidneys to investigate abundance of proximal tubule or medullary thick ascending limb Na^(+)/H^(+) exchanger type 3(NHE3) and collecting duct H^(+)- ATPase using specific polyclonal antibodies to NHE3 and H^(+)-ATPase B1 subunit, respectively. The abundance of NHE3 from cortical homogenates was not changed by either furosemide or hydrochlorothiazide infusion. However, the abundance of NHE3 from outer medullary homogenates was increased by furosemide infusion. The H^(+)-ATPase B1 subunit abundance was increased by furosemide or hydrochlorothiazide infusion in both cortical and outer medullary homogenates. Conculsion : These increases in the abundance of proton-secreting transporters may account for the enhanced distal urinary acidification in response to chronic diuretic administration. (Korean J Nephrol 2002;21(2):222-231)

란크릭 캅셀(플루옥세틴 20mg)에 대한 프로작 캅셀의 생물학적 동등성 시험

심상범,조요나,오한석,류재환,이경태,김남재,서성훈 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Bioequivalence of two flouxetine capsule, ProzacR (Lilly kora Ltd.) and LanclicR (Samsung Pharm. IND. Co.), was evalated according to the guideline of KFDA. Twenty four healthy male volunteers (21-26 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After 60mg of fluoxetine was orally administered, blood was taken at 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 24, 32, 48 and 72 hours after administration and just before administration. Plasma was analyzed for fluoxetine and internal standard (clomipramine) by a sensitive and validated HPLC assay. The pharmaco kinetic parameters (AUCt, Cmax and Tmax) wre calculated and ANOVA test was used for the statistical analysis of parameters. Differences in (AUCt, Cmax and Tmax between two capsules were -0.90, 3.46 and -14.08% respectively. All powers (1-β) for AUGt, Cmax and Tmax were more than 0.9. Detectable differences (Δ) and confidence interval were all less than ±20%. All the parameters above met the criteria of KFDA for bioequivalence and indicated that LanclicR capsules are bioequivalent to ProzacR capsules.

원위부 신세뇨관성 산증에서 산-염기 운반체의 결손

김혜영,한진석,이정상,김현리,김진,이중건,이서진,김근호,진호준,전은실,주권욱,나기영,정우경,오지은,엄재호,궁성수 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.5

The purpose of this study was to elucidate whether the molecular defect of acid-base transporters in renal tubules is related to the functional defect of urinary acidification in distal renal tubular acidosis(RTA). We performed NH₄Cl, furosemide, or bicarbonate loading test to evaluate renal acidification function, and immunohistochemistry using antibodies to H^+ -ATPase, Cl^-/HCO₃^- exchanger(band-3 protein), and Na^+/K^+ -ATPase in kidney tissue in 6 patients with RTA and renal cell carcinoma patients as normal controls. Kidney tissue was obtained either by percutaneous needle biopsy(RTA) or nephrectomy(NC). The results were as follows; 1) In all six RTA patients, proton secretory defect of distal acidification was shown by a failure to lower the urine pH after NHC1 loading or furosemide test or abnormally low urine-blood pCO₂ difference during bicarbonate loading. In two patients with RTA, proximal acidification defect was combined, which was demonstrated by increased fractional excretion of bicarbonate. 2) In mal control, intense H^+ -ATPase and band-3 protein staining was observed in collecting ducts. 3) In distal RTA patients, H6+ -ATPase and band-3 protein staining was not demonstrable or markedly decreased in the intercalated cells of distal nephron. 4) In two patients who had both proximal and distal RTA, H^+ -ATPase staining was markedly decreased in the brush border of proximal tubules as well as the distal nephron. In conclusion, the defect of acid-base transporters in renal tubule was related with the functional defect of urinary acidification in distal RTA.

Metagenomic SMRT Sequencing-Based Exploration of Novel Lignocellulose-Degrading Capability in Wood Detritus from Torreya nucifera in Bija Forest on Jeju Island

( Han Na Oh ),( Tae Kwon Lee ),( Jae Wan Park ),( Jee Hyun No ),( Dockyu Kim ),( Woo Jun Sul ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.9

Lignocellulose, composed mostly of cellulose, hemicellulose, and lignin generated through secondary growth of woody plant, is considered as promising resources for biofuel. In order to use lignocellulose as a biofuel, biodegradation besides high-cost chemical treatments were applied, but knowledge on the decomposition of lignocellulose occurring in a natural environment is insufficient. We analyzed the 16S rRNA gene and metagenome to understand how the lignocellulose is decomposed naturally in decayed Torreya nucifera (L) of Bija forest (Bijarim) in Gotjawal, an ecologically distinct environment. A total of 464,360 reads were obtained from 16S rRNA gene sequencing, representing diverse phyla; Proteobacteria (51%), Bacteroidetes (11%) and Actinobacteria (10%). The metagenome analysis using single molecules real-time sequencing revealed that the assembled contigs determined originated from Proteobacteria (58%) and Actinobacteria (10.3%). Carbohydrate Active enZYmes (CAZy)- and Protein families (Pfam)-based analysis showed that Proteobacteria was involved in degrading whole lignocellulose, and Actinobacteria played a role only in a part of hemicellulose degradation. Combining these results, it suggested that Proteobacteria and Actinobacteria had selective biodegradation potential for different lignocellulose substrates. Thus, it is considered that understanding of the systemic microbial degradation pathways may be a useful strategy for recycle of lignocellulosic biomass, and the microbial enzymes in Bija forest can be useful natural resources in industrial processes.

Antarctic tundra soil metagenome as useful natural resources of cold-active lignocelluolytic enzymes

Han Na Oh,Doyoung Park,Hoon Je Seong,Dockyu Kim,Woo Jun Sul 한국미생물학회 2019 The journal of microbiology Vol.57 No.10

Lignocellulose composed of complex carbohydrates and aromatic heteropolymers is one of the principal materials for the production of renewable biofuels. Lignocellulose-degrading genes from cold-adapted bacteria have a potential to increase the productivity of biological treatment of lignocellulose biomass by providing a broad range of treatment temperatures. Antarctic soil metagenomes allow to access novel genes encoding for the cold-active lignocellulose-degrading enzymes, for biotechnological and industrial applications. Here, we investigated the metagenome targeting cold-adapted microbes in Antarctic organic matter-rich soil (KS 2-1) to mine lignolytic and celluloytic enzymes by performing single molecule, real-time metagenomic (SMRT) sequencing. In the assembled Antarctic metagenomic contigs with relative long reads, we found that 162 (1.42%) of total 11,436 genes were annotated as carbohydrate-active enzymes (CAZy). Actinobacteria, the dominant phylum in this soil’s metagenome, possessed most of candidates of lignocellulose catabolic genes like glycoside hydrolase families (GH13, GH26, and GH5) and auxiliary activity families (AA7 and AA3). The predicted lignocellulose degradation pathways in Antarctic soil metagenome showed synergistic role of various CAZyme harboring bacterial genera including Streptomyces, Streptosporangium, and Amycolatopsis. From phylogenetic relationships with cellular and environmental enzymes, several genes having potential for participating in overall lignocellulose degradation were also found. The results indicated the presence of lignocellulose-degrading bacteria in Antarctic tundra soil and the potential benefits of the lignocelluolytic enzymes as candidates for cold-active enzymes which will be used for the future biofuel-production industry.

Hydrodeoxygenation of oleic acid over Ce<SUB>(1-x)</SUB>Zr<SUB>(x)</SUB>O<SUB>2</SUB> solid solution

Jae-Oh Shim,Dae-Woon Jeong,Won-Jun Jang,Kyung-Won Jeon,Won-Bi Han,Hyun-Suk Na,Hak-Min Kim,Hyun-Seog Roh,Jeong-Geol Na,You-Kwan Oh,Chang Hyun Ko,Sang Sub Han 한국신재생에너지학회 2013 한국신재생에너지학회 학술대회논문집 Vol.2013 No.05

죽염 및 불소용액이 우식병소에 미치는 영향에 대한 미세전산화단 층 촬영술 이용 연구

오한나 ( Han Na Oh ),홍석진 ( Suk Jin Hong ),최충호 ( Choong Ho Choi ) 대한예방치과·구강보건학회 2011 大韓口腔保健學會誌 Vol.35 No.3

Objectives. The purpose of this investigation was to evaluate the remineralization effect of NaF, Bamboo salt and NaF+Bamboo salt solutions on bovine enamel formed incipient artificial enamel caries by microcomputed tomography (micro CT). Methods. Experimental solutions were 2% sodium fluoride solution (2% NaF group), 3% bamboo salt solution (3% BS group) and a mixed solution of 2% sodium fluoride solution and 3% bamboo salt solution (2% NaF+3% BS group). Specimens were prepared from extracted bovine teeth and were divided into 3 groups of 12 specimens each by randomized blocks according to the Vickers hardness number (VHN). The specimens` surfaces were then divided equally into three parts to observe the sound enamel area, the incipient enamel carious area and the remineralized enamel area. Only one-third of each specimen surface was coated with nail varnish and this was exposed to a lactate carbopol buffer system for 72 hours. One-half of the demineralized enamel area was then coated with nail varnish. For remineralization, the specimens were immersed in each experimental solution for 24 hours. After the solution treatment, the VHN and density were measured using a microhardness tester and a micro CT. Results. All experimental solutions showed remineralization effects (p<0.05). The differences of VHN after the experimental solution treatment were statistically significant among the 3 groups (p<0.01). The differences of density after the experimental solution treatment were statistically significant among the 3 groups (p<0.01). The density difference values of the groups were 0.36±0.10 in the 2% NaF group, 0.24±0.06 in the 3% BS group, and 0.35±0.60 in the 2% NaF+3% BS group. Conclusions. The results of the difference of density showed that micro CT could evaluate the remineralization effects of experimental solutions. Thus, it is suggested that a micro CT could be useful for the evaluation of the remineralization effect on incipient carious lesions.