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Mei Jin,Zhiming Liu,Wen Zhang,Haixin Dong,Fang Zhou,Jianfeng Yu,Xinpeng Wang,Zhouyi Guo 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.8
Graphene oxide nanosheet (NGO) was covalently functionalized with positively charged, branched polyethylenimine (bPEI) via an amide bond, coated with serum proteins by electrostatic interaction. Operating as a newly fashioned, multifunctional nanocarrier, the processed NGO showed promise for use in combined gene therapy, chemotherapy, photothermal therapy, bioimaging and as a biosensor of cancer cells. Our current research is focused on the systematic studies of mechanisms of cancer cellular uptake, subcellular location, cytotoxicity and the cellular exclusion of the NGO–bPEI nanocarriers. It was observed that NGO–bPEI accumulated in the mitochondria and that long-term retention of NGO–bPEI led to a decrease in mitochondrial membrane potential while levels of reactive oxygen species increased. The mitochondrial effects associated with long-term retention of NGO–bPEI have potential as a synergistic enhancer of the cytotoxic effects of anti-cancer drugs or genes in human lung carcinoma (A549) cells. This work demonstrated the utility of NGO–bPEI-based multifunctional nanocarriers while detailing the mechanism at the cellular level and providing guidance for further research in cancer therapy.
Cloning, expression and characterization of squalene synthase from Inonotus obliquus
Fangliang Zheng,Nan Liu,Yuchen Che,Li Zhang,Lijie Shao,Junfeng Zhu,Jian Zhao,Haixin Ai,Alan K. Chang,Hongsheng Liu 한국유전학회 2013 Genes & Genomics Vol.35 No.5
The chaga mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. The total triterpene saponins of I. obliquus have significant pharmacological activity. Though the triterpene component has been well characterized in terms of its pharmaceutical activity, there is little information on the genes responsible for the biosynthesis of these compounds in I. obliquus. Squalene synthase represents a potential branching point and the first committed step to diverge the carbon flux from the main isoprenoid pathway towards sterol biosynthesis. In this study, we cloned and characterized squalene synthase from I. obliquus. A 1476-bp full-length cDNA consisting of the entire coding region of squalene synthase (GenBank accession number is KC182754) was cloned by RT-PCR. The DNA sequence showed as much as 76 % similarity with the sequence of Fomitiporia mediterranea squalene synthase, and phylogenetic analysis indicated that it is most closely related to F. mediterranea squalene synthase at both DNA and protein levels. I. obliquus squalene synthase was actively expressed in the yeast Pichia pastoris as a secreted form and purified by gel filtration using Superdex G-75 column. The purified recombinant squalene synthase was able to convert farnesyl diphosphate (FPP) to squalene in an NADPH-dependent reaction. The result of this study could serve as an important step toward the manipulation of triterpenoids biosynthesis in I. obliquus at the level of squalene through engineering better SQS for reintroduction into the mushroom.
Meiqiang Fan,Yong Jin Zou,Hao Yin,Yan Li Huang,Chengqiao Xi,Haixin Kang,Chao Li,Jingjing Zhang,Chunju Lv,Meiqiang Fan,Zhi Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.3
A hollow silicon oxide coated with cuprous oxide and polyaniline (hSiOx/Cu2O/PANI) was prepared via Stober method, magnesium reduction and chemical oxidative polymerization. The hSiOx/Cu2O/PANI (SiOx 62 wt.%, PANI 33 wt.%, Cu2O 5 wt.%) presented charge/discharge capacities of up to 2000 mAhg -1 after 60 cycles at 0.2 Ag -1 current density and higher than 880 mAhg -1 at 8 Ag -1 current density. Microstructural analysis demonstrated that the improvement was due to the nanostructure of hollow SiOx sphere coated with Cu2O and PANI, which could release high stress caused by volume expansion during the lithiation/delithiation process and had little damage to electrode materials. Cycle voltammetry (CV) and electrochemical impedance spectroscopy (EIS) results further confirmed that Cu2O and PANI dual-coating improved reversibility and conductivity of hSiOx and prevented it to drop from the electrode surface.
Ginsenosides Rc, as a novel SIRT6 activator, protects mice against high fat diet induced NAFLD
Zehong Yang,Yuanyuan Yu,Nannan Sun,Limian Zhou,Dong Zhang,HaiXin Chen,Wei Miao,Weihang Gao,Canyang Zhang,Changhui Liu,Xiaoying Yang,Xiaojie Wu,Yong Gao The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.3
Background: Hepatic lipid disorder impaired mitochondrial homeostasis and intracellular redox balance, triggering development of non-alcohol fatty liver disease (NAFLD), while effective therapeutic approach remains inadequate. Ginsenosides Rc has been reported to maintain glucose balance in adipose tissue, while its role in regulating lipid metabolism remain vacant. Thus, we investigated the function and mechanism of ginsenosides Rc in defending high fat diet (HFD)-induced NAFLD. Methods: Mice primary hepatocytes (MPHs) challenged with oleic acid & palmitic acid were used to test the effects of ginsenosides Rc on intracellular lipid metabolism. RNAseq and molecular docking study were performed to explore potential targets of ginsenosides Rc in defending lipid deposition. Wild type and liver specific sirtuin 6 (SIRT6, 50721) deficient mice on HFD for 12 weeks were subjected to different dose of ginsenosides Rc to determine the function and detailed mechanism in vivo. Results: We identified ginsenosides Rc as a novel SIRT6 activator via increasing its expression and deacetylase activity. Ginsenosides Rc defends OA&PA-induced lipid deposition in MPHs and protects mice against HFD-induced metabolic disorder in dosage dependent manner. Ginsenosides Rc (20mg/kg) injection improved glucose intolerance, insulin resistance, oxidative stress and inflammation response in HFD mice. Ginsenosides Rc treatment accelerates peroxisome proliferator activated receptor alpha (PPAR-α, 19013)-mediated fatty acid oxidation in vivo and in vitro. Hepatic specific SIRT6 deletion abolished ginsenoside Rc-derived protective effects against HFD-induced NAFLD. Conclusion: Ginsenosides Rc protects mice against HFD-induced hepatosteatosis by improving PPAR-α-mediated fatty acid oxidation and antioxidant capacity in a SIRT6 dependent manner, and providing a promising strategy for NAFLD.