http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
상황버섯(Phellinus linteus)으로부터 Ergosterol의 분리
류하나,유종수,송명종,이대영,김동현,노영덕,김인호,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
상황버섯을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel 및 octadecylsilica gel(ODS) column chromatography로 정제하여 1종의 sterol 화합물을 분리하였다. 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ergosta-7,22E-diene-3β,5α,6β-triol(cerevisterol, 1)로 동정하였다. 이 화합물은 상황버섯에서 처음 분리 보고 되었다. Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to the isolation of one sterol. From the result of spectral data including NMR, MS and IR, the chemical structure of the sterol was determined as ergosta-7,22E-diene-3β,5α,6β-triol (cerevisterol, 1). Compound 1 was isolated from the Phellinus linteus for the first time in this study.
Production of an antibody for treatment of rheumatoid arthritis using plants
Ha Na Choi,Ki Seong Ko,Jae Yong Yoo,Bich Ngoc Vu,Young Eun Lee,Yoo Na Lee,Mi Hui Jang,Kyun Oh Lee 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Rheumatoid arthritis, which is known as an autoimmune phenomenon as the main mechanism, is a chronic inflammatory disease that causes inflammation in several joints, such as the hands, wrists, feet, and ankles. Humira, a monoclonal antibody, recognizes tumor necrosis factor-alpha (TNF-α) and is used to treat rheumatoid arthritis. Humira is mostly produced in animal cell-based production systems that have high investment costs, high production costs, and risk of contamination. In this study, Humira was produced using plants to overcome the problems of the existing production system. In order to improve the efficacy of the antibody and reduce side effects, glycoengineered plants without plant-specific residues developed in previous studies were used. In addition, in order to solve the low expression level of the recombinant protein, a vector containing the antibody gene was constructed using a virus-based expression system in which expression is regulated by an inducible promoter. The expression vector was introduced into Agrobacterium to transform wild-type plants and glycoengineered plants, and the transformed plants were selected using an antibiotic-containing medium and a reporter gene. The expression of the antibody expressed in the plant was confirmed using an antibody specific to the protein extracted from the leaf. It was identified at a size of approximately 50 kDa and 27 kDa, respectively, representing the heavy and light chains of the antibody. Single copy plants were selected through the segregation test, and homozygous lines were established through the selection process. The expression level of the recombinant protein by the inducible promoter was compared according to the concentration and time of the inducer. These results show the potential for lower production costs and safer production of rheumatoid arthritis drugs using plants.
Production of an enzyme for treatment of Gaucher's disease using plants
Yoo Na Lee,Ki Seong Ko,Jae Yong Yoo,Bich Ngoc Vu,Young Eun Lee,Ha Na Choi,Mi Hui Jang,Kyun Oh Lee 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Gaucher's disease is a metabolic disorder in which a functional deficiency of an enzyme occurs due to a mutation in the gene encoding glucocerebrosidase (GC), resulting in multiple organ malfunctions. Gaucher's disease is being treated with enzyme replacement therapy, which compensates for the enzyme deficiency by administering activated GCs produced in mammalian cells. However, there is a potential of contamination by pathogenic viruses or prions in the mammalian cell-based manufacturing method, as well as a high production cost. In this study, GCs were expressed in plants that produce customized N-glycans to overcome the limitations of the mammalian cell-based production system and to produce a safe treatment drug for Gaucher's disease. A human cDNA library was used to clone a region expressing a GC gene, and sequence analysis was performed. In addition, a binary vector was created and introduced into plants using Agrobacterium to insert the sequence-confirmed GC gene. Plants into which the GC gene was introduced were selected using an antibiotic-containing media, and the presence or absence of the GC gene introduction into the transformed plants was confirmed using polymerase chain reaction (PCR). Furthermore, proteins were collected from the plant leaves into which the gene was inserted, and GC expression levels in the transformed plants were measured using a GC antibody. Finally, a single cloned plant was selected through the antibiotic-resistant isolate ratio. These findings suggest that employing plants, it is possible to overcome the disadvantages of the present mammalian cell-based manufacturing method, decreasing the production cost of pricey Gaucher's disease therapy, and effectively generating safer enzyme treatment.
LC : Evaluation of Adrenal Insufficiency in Cirrhotic Patients in Korea
( Ha Na Yoo ),( Su Rin Shin ),( In Ho Moh ),( Sang Hoon Park ),( Myung Seok Lee ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Adrenal insufficiency (AI) is frequent in cirrhotic patients with severe infection or hemodynamic instability. However, there is limited data about the prevalence of AI in stable cirrhotic patients and the impact of etiology of cirrhosis on AI. This study prospectively evaluated prevalence and characteristic of AI in stable cirrhotic using the conventional short synacthen, a synthetic analogue of adrenocorticotrophic hormone. Methods: From July 2011 to Aug 2012, a total of 50 cirrhotic patients without infections or hemodynamic instability admitted to two university-affi liated hospitals were enrolled. AI was defined by a total serum cortisol level < 18 μg/dL at 30 or 60 min after injection of 250 μg of synacthen. Results: Thirty eight patients were men and age was ranged 29-89 years. Etiology of cirrhosis was alcohol/viral hepatitis/ both/others in 26/6/10/8 patients. AI was present in 18 patients (36%). No significant difference was observed regarding age, gender, mean arterial pressures, and heart rates between patients with and without AI. The etiology of cirrhosis and degree of alcohol consumption did not affect presence of AI or basal and peak serum cortisol levels either. Prothrombin time was higher, while albumin was lower in patients with AI than those without AI. However, in multivariate analysis, there was no independent predictor of AI. The prevalence of AI increased according to severity of liver disease (17, 50, and 33% in Child- Pugh class A, B, and C respectively; P=0.174). There were negative correlations between Child-Pugh score and both basal cortisol (γ=-.377, P=0.007) and peak cortisol levels (γ=-.373, P<0.005) Conclusions: In this study, AI was frequent in stable cirrhotic patients without infections or hemodynamic instability. AI was not related to the etiology of cirrhosis or alcohol consumption, either. AI tended to be associated with only severity of liver disease.