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LiDAR 자료를 이용한 A/R CDM 대상지 선정에 관한 연구
( Cui Gui Shan ),박태진 ( Tae Jin Park ),이우균 ( Woo Kyun Lee ),이종열 ( Jong Yeol Lee ),곽두안 ( Doo Ahn Kwak ),곽한빈 ( Han Bin Kwak ) 대한원격탐사학회 2012 大韓遠隔探査學會誌 Vol.28 No.5
교토메커니즘의 체제하에서 신규조림 및 재조림(Afforestation and Reforestation Clean Development Mechanism, A/R CDM) 사업을 위해서는 대상지적격성 입증이 필요하다. 대상지 적격성 입증을 위해서는 과거에서 현재까지 산림이 아닌 지역으로 규정되어 있으므로 현재 대상지역이 산림 정의에 부합되지 않음을 입증하여야 된다. 본 연구에서는 위성영상을 이용하여 A/R CDM 대상지를 선정을 위한 분류기법을 제시하고자 한다. 연구대상지는 양평군 양평읍의 일부 지역을 선정하였고, 산림정의에 부합하는 3가지 요소 즉, 수고, 울폐도, 면적을 고려하여 LiDAR 자료와 항공사진을 이용하였다. Moving window를 적용하여 수고, 토지면적, 울폐도를 동시에 고려하여 화소기반 산림/비산림 지역으로 분류하였다. 그 결과, 조림 가능지역은 124.06 ha이고 조림 불가지역은 약 357.02 ha이다. 분석에서 적용된 기법은 A/R CDM 사업 대상지 선정 뿐만아니라 기타 교토메커니즘의 활용에 기초방법론을 제공하였다. Verifying about eligibility of targeted site is necessary for execute Afforestation and Reforestation Clean Development Mechanism (A/R CDM) project which is followed by system of Kyoto protocol. The site have to be identified by which could not be in conformity with definition of forest. This study tried to propose a technology of classify for site selection of A/R CDM. We chose several parts of Yangpyeng as study area and applied LiDAR data and remotely sensed imagery for considering about tree height, degree of crown closure, and land area which 3 factors for identify forest. LiDAR data was used for offset the shortage of remotely sensed imagery that cannot perfectly determine the forest definition due to absence of 3-dimentional information, but can be obtained from LiDAR. Considering tree height, degree of crown closure, and land area simultaneously by moving window, classified fields to forest and non forest based on pixel size. As a result, 124.06 ha for suitable to doing plantation and approximately 357.02 ha are in negative. Technology that applied for analyzing will provide fundamental methodology not only site selection for A/R CDM, but will be utilized in other Kyoto protocol.
IKONOS 위성영상을 이용한 중국 장백산 일대의 식생분류 및 바이오매스 추정
CuiGuiShan ( Gui Shan Cui ),이우균 ( Woo Kyun Lee ),ZhuWeiHong ( Wei Hong Zhu ),이종열 ( Jong Yeol Lee ),곽한빈 ( Han Bin Kwak ),최성호 ( Sung Ho Choi ),곽두안 ( Doo Ahn Kwak ),박태진 ( Tae Jin Park ) 한국임학회 2012 한국산림과학회지 Vol.101 No.3
본 연구에서는 접근이 어려운 장백산 중국 지역의 바이오매스를 현장조사 자료와 IKONOS 위성영상을 이 용하여 추정하였다. IKONOS 위성영상을 이용하여 임상단위로 수종구분을 하고, 위성영상으로부터 추정된 식생지수 와 기존 연구에서 추정된 장백산 일부 지역의 바이오매스(Biomass)를 이용하여 회귀분석을 실시하였다. 이때, 위성영 상으로부터 추정된 식생지수 5가지(SAVI, NDVI, SR, ARVI, EVI)와 현장정보가 이용되었다. 그 결과 5가지 식생지 수와 바이오매스간의 상관관계의 결정계수의 순위는 다음과 같이 SAVI(0.84), NDVI(0.73), SR(0.59), ARVI(0.0036), EVI(0.0026) 나타났다. 이와 같은 결과를 바탕으로 최종적으로, 장백산 일부 지역에 대한 수종별 바이오매스 분포량 을 산출함으로써 천연림의 탄소흡수원 추정을 위한 기초자료를 마련하였다. This study was to estimate the biomass of Mt. Changbai mountain area using the IKONOS imagery and field survey data. Then, we prepared the regression function using the vegetation index derived from the IKONOS and biomass estimated from field measured data of previous studies, respectively. The five vegetation index which used in the regression model was SAVI, NDVI, SR, ARVI, and EVI. As a result, the rank of the R-square from coefficient of correlation was as follow, SAVI(0.84), NDVI(0.73), SR(0.59), ARVI(0.0036), EVI(0.0026). Finally, we estimated the biomass of non-measured area using the Soil Adjusted Vegetation Index (SAVI). This study can be used as reference methodology for the estimation of carbon sinks of primary forest.
Zhao, Jun-Qiang,Sun, Fang-Jie,Liu, Shan-Shan,Yang, Jun,Wu, Yu-Quan,Li, Gui-Shan,Chen, Qing-Yong,Wang, Jia-Xiang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Aim: Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC). Methods: Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR. Results: (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis. Conclusion: The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.
Shan-Jing Yao,Yi-Xin Guan,Hai-Xue Pan,Yong-Gui Gao,Man-Gi Cho 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human interferon- (rhIFN-) at a high concentration. The rhIFN- was overexpressed in E. coli, resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded rhIFN-, with protein recovery of 67.1% and specific activity up to 1.2 107 IU/mg.
Gui Miao,Guo Junliang,Kong Huanjun,Wu Pan,Shan Jianqiang,Peng Yujiao 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.9
A phenomenological study on CHF in a bilaterally heated annulus with equal heat flux on both sides was experimentally performed. The working fluid of the present test was R-134a. Variation characteristics of CHF and transition of CHF occurrence location were investigated under different pressure, mass flux and quality conditions. With the increase of critical thermodynamic quality, it was found that CHF first occurred on the outer surface of the annulus, then simultaneously occurred on both sides, and finally occurred on the inner surface at relatively high critical quality. After the CHF location transitioned to the inner rod, the sharp fall of CHF in the limiting critical quality region was observed. The critical quality corresponding to the CHF location transition decreased with the increase of mass flux and pressure. Besides, CHF in tube, internally heated, externally heated and bilaterally heated annuli were compared under the same hydraulic diameter conditions. The present study is conducive to improving the understanding of complicated CHF mechanism in bilaterally heated annulus, enriching the experimental database, and providing evidence for developing accurate CHF mechanism model for annuli.
Shan, Li-Na,Song, Yong-Gui,Su, Dan,Liu, Ya-Li,Shi, Xian-Bao,Lu, Si-Jing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
The zinc finger transcription factor EGR 1 has a role in controlling synaptic plasticity, wound repair, female reproductive capacity, inflammation, growth control, apoptosis and tumor progression. Recent studies mainly focused on its role in growth control and apoptosis, however, little is known about its role in epithelial-mesenchymal transition (EMT). Here, we aim to explore whether EGR 1 is involved in TGF-${\beta}1$-induced EMT in non-smallcell lung cancer cells. Transforming growth factor (TGF)-${\beta}1$ was utilized to induce EMT in this study. Western blotting, RT-PCR, and transwell chambers were used to identify phenotype changes. Western blotting was also used to observe changes of the expression of EGR 1. The lentivirus-mediated EGR 1 vector was used to increase EGR 1 expression. We investigated the change of migration to evaluate the effect of EGR 1 on non-small-cell lung cancer cells migration by transwell chambers. After stimulating with TGF-${\beta}1$, almost all A549 cells and Luca 1 cells (Non-small-cell lung cancer primary cells) changed to mesenchymal phenotype and acquired more migration capabilities. These cells also had lower EGR 1 protein expression. Overexpression of EGR 1 gene with EGR 1 vector could decrease tumor cell migration capabilities significantly after adding TGF-${\beta}1$. These data s howed an important role of EGR 1 in the EMT of non-small-cell lung cancer cells, as well as migration.
Feng-Shan Gao,Xian-zhu Xia,Yu-Wei Gao,Ya-Duo Bai,Xiao-Huan Zou,Gui-Xue Hu 대한수의학회 2009 Journal of Veterinary Science Vol.10 No.3
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.
Zhiyong Li,Shan Zhao,Xiang-Dong XIN,Bei Zhang,Attaribo Thomas,Asakiya Charles,Kwang Sik Lee,진병래,Zhong-Zheng Gui 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
Silkworm (Bombyx mori) pupa protein is one potential source of insect protein for use in food. Immunomodulatory peptides are specific protein fragments that can positively influence human health. Here, we purified a novel immunomodulatory hexapeptide from the alcalase hydrolysate of ultramicro-pretreated silkworm pupa proteinusing Sephadex gel filtration chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide sequence was determined by liquid chromatography–electrospray ionization– tandem mass spectrometry (LC-ESI-MS/MS). The results showed that the molecular mass of the purified peptide was 656.17 Da, and the amino acid sequence was Pro-Asn-Pro-Asn-Thr-Asn (PNPNTN). Splenocyte proliferation was 87.35% in the presence of 100 μg/ml of purified peptide. The splenocyte proliferation could be promoted upto 248.4% at 100 μg/ml of PNPNTN after induction by Concanavalin A (Con A). PNPNTN was stable in the presence of the gastrointestinal proteases pepsin and trypsin and at temperatures up to120°C. Taken together, these results show that this novel immunomodulatory hexapeptide from silkworm pupae has potential therapeutic value as an immunomodulatory component of functional food.