MicroRNA-362 Inhibits Cell Proliferation and Invasion by Directly Targeting SIX1 in Colorectal Cancer

Jin’e Wan,Jian Yang,Cuixia Qiao,Xiaomei Sun,Aiting Di,Lize Zhang,Dandan Wang,Gang Zhao 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.5

Purpose: Colorectal cancer (CRC) is the third most common cancer in China and poses high morbidity and mortality. In recentyears, increasing evidence has indicated that microRNAs played important functions in the occurrence and development of tumors. The purpose of this study was to identify the biological mechanisms of miR-362 in CRC. Materials and Methods: Quantitative real-time PCR was carried out to assess the expression of miR-362 and SIX1. The Kaplan-Meier method was employed to evaluate the 5-year overall survival of CRC patients. The proliferative and invasive abilities of CRCcells were assessed by MTT and transwell assays. Results: miR-362 was significantly decreased in CRC tissues and cell lines, compared to the normal tissues and normal cells. Asignificant connection was confirmed between the overall survival of 53 CRC patients and low expression of miR-362. Downregulationof miR-362 inhibited the proliferation and invasion through binding to the 3'-UTR of SIX1 mRNA in CRC. Additionally, wediscovered that SIX1 was a direct target gene of miR-362 and that the expression of miR-362 had a negative connection with SIX1expression in CRC. SIX1 could reverse partial functions in the proliferation and invasion in CRC cells. Conclusion: miR-362 may be a prognostic marker in CRC and suppress CRC cell proliferation and invasion in part through targetingthe 3'-UTR of SIX1 mRNA. The newly identified miR-362/SIX1 axis provides insight into the progression of CRC.

Dislocation Density-Based Constitutive Model and Processing Map for T2 Copper During Isothermal and Time-Variant Deformation

Gang Chen,Yu Jin,Jing Wang,Cheng Zhang,Qiang Chen,Hongming Zhang,Xingjian Zhao,Zhiyong Li,Changhai Xie,Zhiming Du 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.9

Isothermal compression tests were carried out to study the hot deformation behavior of T2 copper under various conditions. The deformation parameters, such as temperature and strain rate, have strong influence on flow stress and microstructureevolution of the alloy. A unified dislocation density-based model considering dynamic recovery and recrystallization wasestablished. And material parameters of the developed model were optimized by genetic algorithm. Comparisons betweenthe experimental and model data demonstrates that the developed model can precisely describe the flow behavior at quitewide range of deformation conditions. Meanwhile, the designed iterative procedure allows the model to be applied in timevariantdeformation conditions. Processing map and microstructure examination were constructed to optimize the processingwindow of the studied alloy. According to the processing maps, flow instability mainly appeared at low temperatures of500–650 °C and strain rates higher than 0.1 s−1. The optimum deformation parameters of T2 copper was concluded as thetemperature range of 700–800 °C and the strain rate of 0.1–1 s−1.

농업유역 소하천의 T-P,T-N 모니터링

이강석,연규방,박진기,나상일,박종화 충북대학교 농업과학기술연구소 2012 農業科學硏究 Vol.28 No.1

본문참고

이동 소하천의 수온,pH,부유물질량,용존산소,생화학적 산소요구량의 특성

이간석,연규방,박진기,나상일,박종화 충북대학교 농업과학기술연구소 2012 農業科學硏究 Vol.28 No.1

본문참고

비디오 흉강경을 이용한 자연성 기흉 수술에서 재발 방지 방법에 대한 연구

박성강,이석기,임진수,최형호 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background : This study aims to assess the efficacy of VATS pleurodesis in the treatment of spontaneous pneumothroax. Method and material : 131 consecutive patients who underwent videoUassisted thoracoscopy (VATS) bullectomy and pleurodcsis procedure in Chosun Uniservity Hospital between May 1999 and June 2003 were included in the study. Theses patients were performed the VATS with Talc pleurodesis (Group A, n=72), VATS with Doxycycline pleurodesis (Group B, n=36), and VATS with reinforcement by absorbable mesh (Surgicel_(R)) and fibrin glue (Group C, n=23). The follow-up period was from 4 months to 30 years. Result: The patient's population consisted of 117 men and 14 women, and the mean age of the patients was 25.6 years. Recurrence rate was 1.4% ( 1 of 72 patients) in group A, 5.6% (2 of 36 patients) in groug B and 0% (0 of 23 patients) in group C. The median removal period of drainage chest tube was 3.3 days. Conclusion : Careful surgical technique is fundamental in reducing early air leakage and recurrence. Moreover, the covering of the suture area with an absorbable mesh and the fibrin glue is safe and effective method with an acceptable recurrence rate.

BSC 지표들을 이용한 ERP시스템의 성과 측정

김강진,신춘우,김기평 朝鮮大學校 經營經濟硏究所 2007 經營經濟硏究 = Management and economics research Vol.30 No.1

경추디스크 예방을 위한 새로운 형태의 경부베개 개발 및 비교평가

허진강,권봉안 韓國體育大學校附屬 體育科學硏究所 1998 韓國體育大學校附屬 體育科學硏究所論文集 Vol.17 No.1

Objective : Poor head and neck support during sleep can exacerbate the neck pain. Based on the ideal sleep posture and pillow suggested by Cyriax, we designed a new cervical pillow and compared the degree of pain reduction, quality of sleep and pillow satisfaction with a low hospital pillow and a high pillow. Method : The newly designed pillow has a built-in pressured-adjustable air bag in the cervical area and provides normal cervical lordotic curve in supine position and maintains cervical and thoracic vertebrae to form a horizontal line in side-lying position. Thiry-four patients with cervical pain used low hospital pillows for the first week of 3-week randomized crossover design study. They were subsequently randomly assigned to use each of the other two pillows for 1-week period. Outcomes were measured using Visual analog scale. Sleep questionaire, a pillow satisfaction scale. Result : Compared with other 2 types of pillow, Subjects using the newly desinged pillow showed much reduced pain intensity, increased duration of sleep and better satisfaction. Conclustion : We desinged a cervical pillow with built-in pressure adjustable air bag and it can significantly reduce pain intensity and improve quality of sleep in patients with cervical pain.

농업용수 수질기준의 문제점 및 개선대책 : 외국사례와의 비교를 통해

김진호,이종식,정구복,윤순강,고문환,심재천,권순국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

우리나라 농업용수 수질기준과 다른 국가 또는 농업관련 국제기구의 수질기준에 대한 비교분석을 통해 우리나라 농업용수 수질기준의 문제점 도출과 해결방안을 모색코자 본 연구를 수행하게 되었으며, 그 결과를 요약하면 다음과 같다. 1. 우리나라 농업용수 수질기준은 관개용수 수질기준이 아니고, 수원보호측면의 수질기준이라 할 수 있다. 2. 우리나라 수질기준중 유기물지표가 하천과 호소에 따라 BOD와 COD로 이원화되어 있고, 특히 COD의 경우 산화제로 KMnO4를 사용함으로써 효과적인 유기물지표로 활용할 수 없는 실정이다. 농업용수로의 활용측면에서는 K₂Cr₂O_(7)을 이용한 COD를 기준으로 도입하는 것이 유기물 분해능 및 수중의 유기물지표로 활용하고 있는 국제적 데이터 일치를 위해서도 타당하다. 3. 우리나라 농업용수 수질기준은 캐나다와 FAO에서 제시하고 있는 염류에 대한 기준은 거의 없는 실정이다. 또한 양이온에 대한 기준도 없다. 염류의 기준이 모든 이온 존재의 가능성을 의미하므로, 실험치 및 외국문헌에 의한 1.0dS/m수준을 한계농도로 선정해야 한다. 4. FAO와 캐나다에서 사용하고 있는 축산용수에 대한 수질기준이 없다. 5. 관개용 농업용수가 농업(작물)에 미칠 수 있는 영향을 평가하기 위해서는 수원별로 수질기준이 다른 것이 큰 모순이다. 따라서 관계부처에서는 부처간의 이해를 떠난 수질기준을 설정해야 한다. 6. 우리나라 농업용수 수질기준의 모순점을 극복하기 위해서는 농업의 안전성확보측면 위해 관개용수 수질기준과 축산용수 수질기준을 조속히 제정해야 할 것이다. Present problems and possible solutions on the Water Quality Standards for Agriculture in Korea, were identified in the study. Because the Ministry of Environment made the laws and standards without consulting the agri-scientists, among others, the water quality standards formulated were not suitable standards of FAO, Japan, Canada, and the likes. The BOD and COD are the organic materials indicators. In case of rivers, BOD are set for the indicator. And at reservoirs. COD are set. Especially. COD was analysed using KMnO₄It is not suitable oxidizing agent for COD. Because the oxidic capacity is very low. So, in most developed country, K₂Cr₂O_(7) is usually used to analyze COD. And the salts and cations are not belonged to the Korean Water Quality Standards for Agriculture. The water quality standards for agriculture have big differences among lakes, rivers and groundwater. To apply different standards by resources to evaluate the relationship between water and crops is conflicting. So, to overcome the these problems, the suitable water quality standards for irrigation and livestock should be set.

Sr· 페라이트의 자기특성에 미치는 Al₂O₃의 영향에 관한 연구

신용진,서강수,정병두,김형진 明知大學校 産業技術硏究所 1991 産業技術硏究所論文集 Vol.10 No.-

In this thesis, We have measured the effect of Al₂O₃ to the magnetic properties of Sr-ferrite. The specimens have been composed of SrO and Fe₂O₃. Ratios of 1:5.4 to 1:5.8 have been chosen for SrO and Fe₂O₃. As additives, We used 1wt%, 2 wt% and 3 wt% of Al₂O₃. Sintering was carried out at 1250℃ . From experiments on the magnetic properites of specimen, the results are as follows; 1) For SrO : Fe₂Op3 = 1 : 5.4 and 1 : 5.8, we got similar coercivity and residual flux density, but for 1 : 5.6, decrease sharply. 2) For the additive Al₂O₃, coercivity is increased and residual flux density is decreased with Al₂O₃ respectively. Good basic composition region of nonstoichiometric Sr-ferrite has been appeared on the n=5.4 and n=5.8, but in the case of Al₂O₃ addition, We have confirmed good magnetic properties on the n=5.8.

Lipopolysaccharide 로 유발된 생쥐 무릎관절낭 염증에 관한 형태학적 연구 : 윤활관절막과 섬유관절막의 변화를 중심으로 Based on the Morphological Changes of Synovial Membrane and Fibrous Membrane

김진택,안상현,최난희,정재만,박인식,강윤호,김호현,이해풍 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 관절염 유발시 일어나는 관절낭의 형태학적 변화를 조사하기위해 ljpopolysaccharide(LPS)주사로 인위적 관절낭 염증을 유발시킨 후 시간경과에 따른 윤활관절막과 섬유관절막의 형태 변화를 관찰하였다. BALB/C 암컷 생쥐 오른쪽 무릎관절낭에 LPS 300㎍/㎏를 주사한 후 3, 7 그리고 14일에 무릎관절을 얻었다. 무릎관절은 4주동안 EDTA용액에 탈회한 후 통상적 방법으로 paraffin에 포매하였다. 또한 윤활관절막의 미세구조변화는 embed812로 포매한 후 관찰하였다. LPS 주사후 관절연골 인접부위의 윤활관절막에서 시작된 세포과형성(hyperplasia)은 시간 경과후 전체 윤활관절막으로 확대되었다. 윤활관절막내의 미세구조의 변화로는 윤활포식세포(type 1)가 관절강내로의 많은 돌기(filopodia)를 내었고, 잘 발달된 과립형질내세망을 가지는 type 2 윤활분비세포의 숫적 증가가 보였다. 한편 LPS 주사후 섬유관절막에서 나타나는 형태학적 변화는 collagen fiber 생성에 의한 섬유화가 증가되며, 이러한 섬유화를 주도하는 섬유모세포의 이주증가파 관찰되었다. 또한 혈관 주위에서는 백혈구의 이주 증가가 나타났으며, 탈과립형(degranulated type) 비만세포가 많이 관찰되었다. 이상의 결과로 LPS 주사로 관절낭에서 염증이 유발되어 윤활관전막과 섬유관절막에서 형태학적 변화가 나타났다. 이러한 일련의 형태학적 변화는 발병초기 류마티스성 관절염에서 나타나는 병리학적 소건과 동일한 결과로서, 앞으로 진행될 치료제 개발과 유발기전에 관한 해석을 위한 in vivo 실험의 적절한 모델로 기여한 것으로 기대된다. Synovial joint of BALB/C mice were injeced with Lipopolysaccharide(LPS) were observed to investigate the morphological changes of synovial capsule caused by rheumatoid arthritis(RA). The RA on female Balb/c mice were induced by LPS injection, as dose of 300㎍/㎏, into synovial cavity of knee joint. And then these specimen were fixed in 10% neutral buffered formalin and were decalcificated in EDTA solution for 4 weeks. The hyperplasia of synovium were appeared in synovial membrane. The filopodia of phagocytic like synoviocyte(type Ⅰ synoviocyte) projected into synovial cavity and the number of fibroblast like synoviocyte(type Ⅱ synoviocyte) with well-developed endoplasmic reticulum were increased in synovium. In fibrous membrane, the fibrosis induced by synthesis of collagen fiber were enlarged to all fibrous membrane, and the number of fibroblast were increased. A great number of inflammation component cell as Iymphocyte and neutrophil leukocyte were infiltrated around capillary and the degranulate typed mast cell were increased. As results indicated that the hyperplasia of synovium induced by LPS, subsequently to cause the fibrosis, infiltration of imflammation component cell, and increase of degranulated type mast call as same as symptoms of RA.