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      • SCIESCOPUSKCI등재

        Effect of the Transformed Lactobacillus with Phytase Gene on Pig Production Performance, Nutrient Digestibility, Gut Microbes and Serum Biochemical Indexes

        Yin, Q.Q.,Chang, J.,Zuo, R.Y.,Chen, L.Y.,Chen, Q.X.,Wei, X.Y.,Guan, Q.F.,Sun, J.W.,Zheng, Q.H.,Yang, X.,Ren, G.Z. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

        In order to improve the availability of phytase and probiotics together, a phytase gene from Aspergillus ficuum has been expressed in Lactobacillus. In this study, the transformed Lactobacillus with phytase gene was fed to pigs to determine its effect on pig production, feed conversion and gut microbes. Forty eight, 60-day-old, castrated pigs (Duroc${\times}$Landrace${\times}$Pietrain) were assigned to 6 groups, 8 pigs for each group. Group 1 was the control, group 2 was added with chlortetracycline (500 mg/kg), group 3 was added with the transformed Lactobacillus (500 mg/kg) with 20% (w/w) of calcium monohydrogen phosphate (CMP, $CaHPO_{4}$) removed, group 4 was added with the natural Lactobacillus (500 mg/kg) with 20% (w/w) of CMP removed, group 5 was added with the transformed Lactobacillus (500 mg/kg) with 40% (w/w) of CMP removed, group 6 was added with phytase (500 mg/kg) with 40% (w/w) of CMP removed. The results showed: i) the average daily gain (ADG) was improved in groups 2, 3 and 4 (p<0.05); ii) the diarrhea rates in the groups added with Lactobacillus were lower than in the other groups (p<0.05), in which the transformed Lactobacillus had more effect on reducing digestive disease; iii) the transformed Lactobacillus was most effective in improving the digestibilities of crude protein (CP), calcium (Ca), phosphorus (P), compared with the other groups (p<0.05); iv) Lactobacillus could increase lactic acid bacterium number and ammonia concentrations, and decrease pH values and E. coli number in pig feces (p<0.05); v) the phytase activity in the feces of pigs fed with the transformed Lactobacillus was 133.32 U/g, which was higher than in group 4 (9.58 U/g, p<0.05), and was almost the same as group 6 (135.94 U/g); vi) the transformed Lactobacillus could increase serum concentrations of IgA, triglyceride, and glutamic oxaloacetic transaminase activity (p<0.05), and had no significant effect on other serum indexes (p>0.05).

      • SCIESCOPUSKCI등재

        Selection for Duration of Fertility and Mule Duck White Plumage Colour in a Synthetic Strain of Ducks (Anas platyrhynchos)

        Liu, H.C.,Huang, J.F.,Lee, S.R.,Liu, H.L.,Hsieh, C.H.,Huang, C.W.,Huang, M.C.,Tai, C.,Poivey, J.P.,Rouvier, R.,Cheng, Y.S. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.5

        A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie ($h^2=0.07{\pm}0.03$) and M ($h^2=0.07{\pm}0.01$), moderately low for Dm ($h^2=0.13{\pm}0.02$), of medium values for H ($h^2=0.20{\pm}0.03$) and F ($h^2=0.23{\pm}0.03$). High and favourable genetic correlations existed between F and Dm ($r_g=0.93$), between F and H ($r_g=0.97$) and between Dm and H ($r_g=0.90$). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings' feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

      • Indirect measurement ofsin2θW(orMW) usingμ+μ−pairs fromγ*/Zbosons produced inpp¯collisions at a center-of-momentum energy of 1.96 TeV

        Aaltonen, T.,Amerio, S.,Amidei, D.,Anastassov, A.,Annovi, A.,Antos, J.,Apollinari, G.,Appel, J. A.,Arisawa, T.,Artikov, A.,Asaadi, J.,Ashmanskas, W.,Auerbach, B.,Aurisano, A.,Azfar, F.,Badgett, W.,Bae American Physical Society 2014 PHYSICAL REVIEW D - Vol.89 No.7

        <P>Drell-Yan lepton pairs are produced in the process p (p) over bar -> mu(+)mu(-) + X through an intermediate gamma*/Zboson. The forward-backward asymmetry in the polar-angle distribution of the mu(-) as a function of the invariant mass of the mu(+)mu(-) pair is used to obtain the effective leptonic determination sin(2)theta(lept)(eff) of the electroweakmixing parameter sin(2)theta w, from which the value of sin(2)theta w is derived assuming the standard model. The measurement sample, recorded by the Collider Detector at Fermilab (CDF), corresponds to 9.2 fb(-1) of integrated luminosity from p (p) over bar collisions at a center-of-momentum energy of 1.96 TeV, and is the full CDF Run II data set. The value of sin(2)theta(lept)(eff) is found to be 0.2315 +/- 0.0010, where statistical and systematic uncertainties are combined in quadrature. When interpreted within the context of the standard model using the on-shell renormalization scheme, where sin(2)theta w = 1 - M-W(2)/M-Z(2), the measurement yields sin(2)theta w = 0.2233 +/- 0.0009, or equivalently a W-boson mass of 80.365 +/- 0.047 GeV/c(2). The value of the W-boson mass is in agreement with previous determinations in electron- positron collisions and at the Tevatron collider.</P>

      • SCIESCOPUSKCI등재

        Estimation of Genetic Parameters for Direct, Maternal and Grandmatemal Genetic Effects for Birth, Weaning and Six Month Weights of Hanwoo (Korean Cattle)

        Choi, S.B.,Lee, J.W.,Kim, N.S.,Na, S.H.,Keown, J.F.,Van Vleck, L.D. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.2

        The objectives of this study of Hanwoo (Korean Cattle) were 1) to estimate genetic parameters for direct and maternal genetic effects for birth weight, weaning weight, and six months weight which can be used for genetic evaluations and 2) to compare models with and without grandmatemal effects. Data were obtained from the National Livestock Research Institute in Rural Development Administration (RDA) of Korea and were used to estimate genetic parameters for birth weight (BW, n=10,889), weaning weight at 120-d (WW, n=8,637), and six month weight (W6, n=8,478) in Hanwoo. Total number of animals in pedigrees was 14,949. A single-trait animal model was initially used to obtain starting values for multiple-trait animal models. Estimates of genetic parameters were obtained with MTDFREML using animal models and derivative-free REML (Boldman et al., 1995). Estimates of direct heritability for BW, WW, and W6 analyzed as single-traits were 0.09, 0.03, and 0.02 from Model 3 which included direct and maternal genetic, maternal permanental environmental effects, and effects due to sire ${\times}$ region ${\times}$ year-season interaction, respectively. Ignoring sire ${\times}$ region ${\times}$ year-season interaction effect in the model (Model 2) resulted in larger estimates for direct heritability than for Model 3. Estimates of maternal heritability for BW, WW and W6 were 0.04, 0.05, and 0.07 from Model 3, respectively. The estimates of direct-maternal genetic correlation were positive for BW, WW, and W6 with Model 3 but were negative with Model 2 for WW and W6. Estimates of direct genetic correlations between BW and WW, BW and W6, and WW and W6 were large: 0.52, 0.45, and 0.90, respectively. Genetic correlations were also large and positive for maternal effects for BW with maternal effects for WW and W6 (0.69 and 0.74), and even larger for WW with W6 (0.97). The log likelihood values were the same for models including grandmatemal effects as for models including maternal effects for all traits. These results indicate that grandmatemal effects are not important for these traits for Hanwoo or that the data structure was not adequate for estimating parameters for a grandmatemal model.

      • KCI등재

        Selection for Duration of Fertility and Mule Duck White Plumage Colour in a Synthetic Strain of Ducks (Anas platyrhynchos)

        H.C. Liu,J.F. Huang,S.R. Lee,H.L. Liu,C.H. Hsieh,C.W. Huang,M.C. Huang,C. Tai,J.P. Poivey,R. Rouvier,Y.S. Cheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.5

        A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie (h2 = 0.07±0.03) and M (h2 = 0.07±0.01), moderately low for Dm (h2 = 0.13±0.02), of medium values for H (h2 = 0.20±0.03) and F (h2 = 0.23±0.03). High and favourable genetic correlations existed between F and Dm (rg = 0.93), between F and H (rg = 0.97) and between Dm and H (rg = 0.90). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings’ feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

      • KCI등재

        Rapid Synthesis of W–Cr Solid–Solution by Dielectric-Barrier Discharge-Plasma-Assisted Ball Milling

        S. F. Hong,Z. C. Lu,Y. H. Liu,Q. M. He,W. Jiang,M. Q. Zeng 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

        Supersaturated solid–solutions in a W–Cr alloy system were fabricated by conventional milling and plasma milling. The differentextension mechanisms of the solid solubility that were created by ball milling with and without discharge plasma werestudied. The solid–solubility of Cr in W for the P-milling W–Cr alloy system was higher, which indicates that plasma millingpromotes the formation of W(Cr) solid–solution more easily than conventional milling. According to Miedema’s model, inthe W–Cr alloy system, the free-energy change to form a W(Cr) solid solution by plasma milling is reduced compared withconventional milling because of the heating effect of the discharge plasma. Therefore, a higher stored energy in the grainboundaries and dislocations of the W–Cr nanograins during plasma milling makes it easier to overcome the thermodynamicbarrier in the formation of a solid–solution, when compared with conventional milling.

      • SCIESCOPUSKCI등재

        Effects of Crystalline Lysine, Threonine and Tryptophan Supplementation of Diets Containing Reduced Protein Levels on Performance of Growing Pigs

        Li, D.F.,Xiao, C.T.,Kim, J.H.,Cho, W.T.,Han, In K. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.1

        Three hundred and fifty-two growing pigs were utilized in three growth trials to investigate the effects of crystalline amino acid supplementation on growing pigs' growth performance and feed consumption. In Exp. 1, diets were formulated on the basis of digestible amino acids. Utilization of rapeseed meal plus cottonseed meal (R + C) in diet supported similar average daily gain (ADG), feed/gain (F/G) and cost per gain (p > 0.05) to group of soybean meal (SBM). In Exp. 2, 14% CP diets containing equal amount of cottonseed meal and rapeseed meal were formulated with the addition of threonine and/or tryptophan. Supplementation with threonine improved ADG and F/G (p < 0.05). Supplementation with only tryptophan made no benefits for ADG of growing pigs. In Exp. 3, diets with different threonine level were formulated. Increasing dietary threonine to 0.54% improved (p < 0.05) ADG and F/G compared to 0.45% dietary threonine. Increase in dietary threonine did not improve ADG (p > 0.05) but decreased F/G (p < 0.05) because of a decrease in average daily feed intake. Pigs fed diet with dietary threonine/lysine ratio of 67.5% supported optimum growth performance.

      • Search for a low-mass neutral Higgs boson with suppressed couplings to fermions using events with multiphoton final states

        Aaltonen, T.,Amerio, S.,Amidei, D.,Anastassov, A.,Annovi, A.,Antos, J.,Apollinari, G.,Appel, J. A.,Arisawa, T.,Artikov, A.,Asaadi, J.,Ashmanskas, W.,Auerbach, B.,Aurisano, A.,Azfar, F.,Badgett, W.,Bae American Physical Society 2016 Physical Review D Vol.93 No.11

        <P>A search for a Higgs boson with suppressed couplings to fermions, h(f), assumed to be the neutral, lower-mass partner of the Higgs boson discovered at the Large Hadron Collider, is reported. Such a Higgs boson could exist in extensions of the standard model with two Higgs doublets, and could be produced via p (p) over bar -> H(+/-)h(f) -> W*h(f)h(f) -> 4 gamma + X where H (+/-) is a charged Higgs boson. This analysis uses all events withat least three photons in the final state from proton-antiproton collisions at a center-of-mass energy of 1.96 TeV collected by the Collider Detector at Fermilab, corresponding to an integrated luminosity of 9.2 fb(-1). No evidence of a signal is observed in the data. Values of Higgs-boson masses between 10 and 100 GeV/c(2) are excluded at 95% Bayesian credibility.</P>

      • SCISCIESCOPUS
      • KCI등재

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