Chlorambucil이 Mouse의 정소에 미치는 영향; 누적투여에 의한 정소의 형태적 변화

全德勳,朴元學 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

The present studies were designed to investigate the effects of chlorambucil on the testis of mouse. Chlorambucil suspended in 0.5N sodium bicarbonate(pH8.0) was administered to mouse at doses of 24mg/Kg/day for 7 to 14 days. The morphological changes in testis of mouse were observed oon 7th day and 14th day of administration by light microscope. After 7 days of chlorambucil administeration, spermatogenesis was partially ceased and interstitial space was moderately reduced. Almost all tubules were slightly damaged and some tubules were severely damaged. The shape of seminiferous tubule was waved circle, but the sertoli and Leydig cell was intact. After 14 days. all spermatogonia cells had disappeared and spermatogenesis was completely ceased. Almost all tubules were severely damaged and the germinal epithelium was markedly decreased. The interstitial space was markedly reduced and the shaped of seminiferous tubule was polygon. But, the sertoli and Leydig cell was no affected.

수컷 생쥐의 정자두부 미세구조에 대한 Chlorambucil의 영향

김덕훈,고복현 동국대학교 경주대학 1990 論文集 Vol.9 No.-

The study was carried out to investigate the effect of Chlorambucil on the mouse sperm head by electron microscopy. Chlorambucil was injected intraperitoneum at a dosage of level 16mg/kg for one weeks, 3weeks and 5weeks, respectively. The result obtained were as follows 1. One week after the administration, Most plasma membrane of late spermatid were swollen and dilation. and the acrosomal membrane was normal. 2. After 3 weeks, the condensation of nucleus was presented at some late spermatids. The nucleus membrane was mostly loss, But the dilation of nucleus membrane was partly observed. The acrosome and the plasma membrane was mostly disrupted. 3. After 5 weeks, most nucleus membrane, acrosome membrane, acrosome, and necleus membrane were severely disrupted. The nucleus was severely condensed and the shape of nucleus was very queer. But, in some late spermatids, the heterochromathin was observed. The membrane whorl in acrosome region was presented at some late spermatids.

비 방사선 in situ hybridization과 confocal scanning microscope를 이용한 HCV에 감염된 human liver cell에서의 mRNA의 연구

정선우,김덕훈 國立昌原大學校 基礎科學硏究所 1994 基礎科學硏究所論文集 Vol.6 No.-

'In situ hybridization' has been used in searching the specific nucleic acid of the mRNA and viral DNA in the cell and tissue. For in situ hybridization, the use of a non-radioactive probe was considered as a good method. Because, the method provided excellent resolution of probe stability and personal safety. This study was performed by using the T-T dimered DNA as the non-radioactive probe in the human liver tissue infected by hepatitis C virus (HCV). The results were as follows. 1. The positive reaction of specific mRNA was detected from the hepatocyte. 2. Using the confocal scanning microscope and IBAS, the positive reaction wite was very evident. But, the negative reaction was not appeared in several cells. 3. Among Kupffer's cells, endothelial cells, and other cells, some were positively stained. 4. The sinusoids, central veins, hepatic arteries, bile ducts and connective tissues presented the negative reaction. 5. In the hepatocyte, the positive reaction was appeared to heterogenous in the nucleus. ‘In situ hybridization' has been used in searching the specific nucleic acid of the mRNA and viral DNA in the cell and tissue. For in situ hybridization, the use of a non-radioactive probe was considered as a good method. Because, the method provided excellent resolution of probe stability and personal safety. This study was performed by using the T-T dimered DNA as the non-radioactive probe in the human liver tissue infected by hepatitis C virus (HCV). The results were as follows. 1. The positive reaction of specific mRNA was detected from the hepatocyte. 2. Using the confocal scanning microscope and IBAS, the positive reaction site was very evident. But, the negative reaction was not appeared in several cells. 3. Among Kupffer's cells, endothelial cells, and other cells, some were positively stained. 4. The sinusoids, central veins, hepatic arteries, bile ducts and connective tissues presented the negative reaction. 5. In the hepatocyte, the positive reaction was appeared to heterogenous in the nucleus.

週齡에 따른 생쥐 Leydig세포의 전자현미경적 구조

서의수,박매자,김덕훈,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.3

주령(週齡)의 증가에 따른 생쥐 Leydig세포의 광학현미경형태와 초미세구조에 관한 변화는 다음과 같다. 1. 생후 1주군에서 미분화된 Leydig세포는 정세관 주위에서 관찰할 수 있었고, 핵은 방추형이며, 핵질과 핵막에는 이염색질이 많이 분포하고, 세포질에는 소포체와 사립체가 조금 분포하나 그 이외의 세포소기관은 거의 없었다. 2. 3주군에서의 Leydig세포는 아직 완전히 분화되지는 않았으나 정세관 주위에서 관찰되었다. 핵은 난형이며, 핵막의 일부 함입과 이염색질의 부착이 있고 세포질에는 잘 발달된 무과립성 내형질망과 membrane whorl이 있으나 그 이외 세포소기관의 발달은 미약하였다. 3. 5주군에서는 완전히 분화한 Leydig세포들의 군집이 정세관 주위에서 관찰되었다. 핵은 거의 원형이며, 핵에는 진염색질이 분포하며, 세포질에는 사립체의 발달이 현저하고, 그 이외의 세포소기관은 약간 증가하였다. 4. 7주군에서는 잘 발달된 정세관의 구조와 잘 분화된 Leydig세포들의 군집을 tubule의 각 공간에서 관찰할 수 있었다. 세포는 핵이 원형으로, 핵막은 뚜렷하고 세포질에는 무과립성 내형질망, 지방소적, 용해소체, 사립체, 당원입자 등이 아주 잘 발달되어 있었다. This study was performed to investigate the ultrastructure of the Leydig cell on aging of the mouse. The results obtained were as follows. One week after birth: The undifferentiated Leydig cells are found around seminiferous tubules. The nucleus showed the fusiform shape. The heterochromatin was found to be adherent to the nuclear membrane and to be dispersed in the nucleoplasm. The smooth endoplasmic reticulum and mitochondria were poorly developed, and the other cell organelles did not appear at this stage. The cytoplasmic vacuolations began to appear. After 3 weeks: Not fully differentiated Leydig cells are present around tubules. The nucleus had oval shape, and some nuclear membrane was caved and adhered to the heterochromatin. The smooth endoplasmic reticulum and membrane was caved and adhered to the heterochromatin. The smooth endoplasmic reticulum and membrane whorl were well developed. But the other cell organelles were poorly developed. After 5 weeks: Clusters of fully differentiated Leydig cells are found around the tubules. The nucleus had round shape, and the nucleoplasm included an euchromatin. Numerous mitochondria were ovserbed at this stage. After 7 weeks: Clusters of fully differentiated Leydig cells are located in the interspace of each tubules. The shape of nucleus was round, and nuclear membrane was prominent. The smooth endoplasmic reticulum, lipid droplets, lysosomal dense body, mitochondria, ribosome and glycogen were increased markedly in number.

Chlorambucil이 수컷 생쥐의 Sertoli 세포의 미세구조에 미치는 영향

최인장,장성익,김덕훈,이인환 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

This study was investigated the effect of chlorambucil (Leukeran) on the Sertoli cell of male mouse by electron microscope. Chlorambucil suspended in the 0.5N sodium bicarbonate (pH 8.0) was injected into the male mouse by intraperitoneal at doses level (16mg/kg) for one week, 3 weeks, respectively. The results were as follows; 1. One week after adminstration of chlorambucil, swelling and their inner cristae distruption of some mitochondria, mild vacuolation of cytoplasm, moderate dilation of smooth endoplasmic reticulum (SER) were presented. But, lipid droplet and secondary lysosome were severely increased. 2. After 3 weeks, the dilation of SER and vacuolation of some cytoplasm, the swelling and inner cristae destruption of most mitochondria were appeared. The lipid droplet and lysosome were midly increased. 3. After 5 weeks, most mitochondria were swelling and their membrane were almost disrupted. The dilation of SER and vacuolation of most cytoplasm were almost severely increased. But lipid droplet and lysosome were not observed. As a results, the duration of the chlorambucil administration is longer, the degeneration of the cytoplasm organelles is increased in comparison with control group. On the other hand, nucleus is not degenerated.

한국산 잠자리과(잠자리목) 5종의 세포분류학적 연구

李鐘郁,愼鏞直,朴元學,金德勳 嶺南大學校附設 基礎科學硏究所 1988 基礎科學硏究 Vol.8 No.-

The chromosome study was performed in some species on the Korean Libellulidae by using squash techniques and lactopropionic orcein stain in order to define species relationship between them. The numerical and morphological differences were shown in the chromsome between the species of the stages of spermatocyte metaphase. The results obtained were summarized as follows: 1. The pattern of standard chromosome number in these species is divided into two groups; Orthetrum albistylum speciosum (UHLER), Orthetrum triangrlare melanina (SELYS), Rhyothemis fulignosa HAGEN are 13 in the haploid chromosome number. But, Crocothemis servilia servilia DRURY and Synoetrum eroticum (SELYS) are 12. 2. The shape of chromosome in these specie in divided into two groups; O. albistylum, S. eroticum eroticum, R. fulignosa, O. triangulare melania are bar or clover in shape at the spermatocyte metaphase. These species seem to be holocentric chromosome. On the other hand, C. servilia servilia is gloval and this species has monocentric chromosome. 3. The sex chromosome of five species ( O. albistylum speciosum, O. Triangulare melania, R. fulignosa, C. servilla servilia, S. eroticum eroticum ) are XO type. 4. Three species ( O. albistylum speciosum, O. Triangulare melania, S. eroticum eroticum ) shows the fragment chromosome in the spermatocyte metaphase. As the results, five species on the Korean Libellulidae (O. albistylum speciosum, O. triangulare melania, R. fulignosa, C. servilla servilia, S. eroticum eroticum ) is similar to each other in the external morphology, But, in the analysis of chromosome, these species is different to some degree.

A Clinical Study of Korean Wearers of the Pure Vision Contact Lens

( Douk Hoon Kim ),( Stanley Crossman ) 한국안광학회 2006 논문집 Vol.2006 No.-

Color soft contact lens and corneal thickness

Kim, Douk-Hoon The Korean Society of Clinical Health Science 2019 한국임상보건과학회지 Vol.7 No.1

Purpose: To investigate the effect of color soft contact lenses on the corneal thickness in young Korean population. Methods: The subjects consisted of 112 eyes (7 males, 49 females) with the mean age of $20.987{\pm}1.093years$ (range 20-25 years) in young Korean population during 2018. Test was compared the corneal thickness before and after wearing color soft contact lenses. The thinnest cornea thickness(TCT), central cornea thickness(CCT), pupil centre thickness(PCT), superior corneal thickness(SCT),inferior corneal thickness(ICT), medial corneal thickness(MCT), lateral corneal thickness(LCT) of the cornea was measured using the Pentacam pachymetry. The statistically analysis was perform the Shaparo-Wilk test. Results: The right eye was a statistically significant among the CCT,LCT, MCT and TCT values(p<0.001) in the compared the corneal thickness before and after wearing soft colour contact lenses. Also the left eye was a statistically significant among the LCT and MCT values(p<0.001) in the compared the corneal thickness before and after wearing soft colour contact lenses. However, there was no statistical difference (p>0.5) in the mean PCT between before and after wearing color soft contact lenses. Conclusion: These results suggested that the color soft contact lens wear can the effect the regional thickness of cornea. Therefore, the analysis of corneal topography with Pentacam can provide correct and useful diagnostic information of the morphology of the RGP contact lens fitting and diagnosis of corneal refraction surgery.

Analysis on the corneal thickness and anterior chamber depth of the Keratoconus using Pentacam

Kim, Douk-Hoon,Sapkota, Kishor The Korean Society of Clinical Health Science 2018 한국임상보건과학회지 Vol.6 No.1

Purpose: To investigate the corneal thickness and anterior chamber depth (ACD) of eyes with Keratoconus in the Korean population with the Pentacam. Methods: The subjects consisted of 84 eyes from Keratoconic adults aged 7-59 years during 2010. The thinnest area, apex zone, and pupil centre of the corneal thickness were measured using the Pentacam pachymetry. ACD value was also measured with Pentacam. Results: There was a statistically significant relationship between thickness of the cornea at the apex area and the pupil centre (p=0.0001). However, there was no statistical difference (p>0.5) in the mean thickness of thinnest area, apex zone, pupil centre of cornea between right eye and left eye. Also, correlation between ACD and corneal thickness in all subjects had no statistical differences (p>0.05) in all subjects. Conclusion: These results suggested that the regional thickness of cornea and ACD with Pentacam can provide correct and useful diagnostic information of the morphology of Keratoconus for the RGP contact lens and diagnosis of abnormal corneal refraction surgery.

양안에서 정상 단안시와 약시안의 P-VEP 분석

김덕훈(Douk Hoon Kim),김규수(Gyu Su Kim),성아영(A Young Sung),박원학(Won Hak Park) 한국안광학회 2005 한국안광학회지 Vol.10 No.1