Microstructure and Mechanical Properties of Cold Drawn Ti–Nb–Ta–Zr–O Wires for Orthodontic Applications

Wei‑dong Zhang,Junye Ren,Bin Liu,Yong Liu,Zhenggang Wu,Jingwen Qiu 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.7

Ti–36Nb–2Ta–3Zr–0.35O (TNTZO) alloy is an excellent candidate for biomedical applications. In this study, a new methodcombining cold-swaging and cold-drawing was used to fabricate the TNTZO alloy wires with 0.3 mm diameter for orthodonticapplications. The microstructure and mechanical properties of cold-drawn and annealed TNTZO wires (referred toas TNTZO0.3and TNTZO0.3(HT), respectively) were investigated. The results show that the microstructure of cold drawnTNTZO0.3consists of main-sized elongated grains with 70 nm width. After annealing at 700 °C for 5 min, the microstructureof TNTZO0.3(HT) wires becomes equiaxial with a grain size of ~ 5 μm. The cold drawn TNTZO0.3wires exhibit improvedmechanical properties, higher tensile strength (about 1000 MPa) and similar elastic modulus (69 GPa), compared to annealedTNTZO0.3(HT) wires. Besides, TNTZO0.3has higher creep resistance and lower stress exponent (around 2), compared to Tiwires and TC4 wires with the same diameter. These results prove that TNTZO0.3wires have most of the ideal characteristicsof orthodontic wires.

Frequency Tracking of Power Grid based on Phase Difference and UKF

Wei Dong,Wei Dong,Shichao Jiang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12

For frequency tracking problem of non-stationary signals in power grid, we propose a unscented Kalman filter (UKF) and the weighted phase difference smoothing algorithm. It improved poor initial conditions sensitive situations of robustness UKF. In order to improve the accuracy of the algorithm, real-time and noise immunity, a suboptimal multiple fading factor and weighted smoothing method is added to improve UKF for frequency tracking of power signals. At last, The simulation results are given. It shows that this method has better accurate, real-time tracking of various frequency power signal and its performance is superior to similar documents tracking algorithm.

Analysis the role of arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis

Dong-Wei Di,Lei Wu,Pan Luo,Li Zhang,Tian-Zi Zhang,Xue Sun,Shao-Dong Wei,Chen-Wei An,Guang-Qin Guo 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

The crosstalk between auxin and cytokinin (CK) is important for plant growth and development, although the underlying molecular mechanisms remain unclear. Here, we describe the isolation and characterization of a mutant of Arabidopsis Cytokinin-induced Root Curling 6 (CKRC6), an allele of ANTHRANILATE SYNTHASE ALPHA SUBUNIT 1 (ASA1) that encodes the á-subunit of AS in tryptophan (Trp) biosynthesis. The ckrc6 mutant exhibits root gravitropic defects and insensitivity to both CK and the ethylene precursor 1-aminocyclopropane-1-carboxylicacid (ACC) in primary root growth. These defects can be rescued by exogenous indole-3-acetic acid (IAA) or tryptophan (Trp) supplementation. Furthermore, our results suggest that the ckrc6 mutant has decreased IAA content, differential expression patterns of auxin biosynthesis genes and CK biosynthesis isopentenyl transferase (IPT) genes in comparison to wild type. Collectively, our study shows that auxin controls CK biosynthesis based on that CK sensitivity is altered in most auxin-resistant mutants and that CKs promote auxin biosynthesis but inhibit auxin transport and response. Our results also suggest that CKRC6/ASA1 may be located at an intersection of auxin, CK and ethylene metabolism and/or signaling.

Biotransformation of Panax ginseng extract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry

Dong, Wei-Wei,Zhao, Jinhua,Zhong, Fei-Liang,Zhu, Wen-Jing,Jiang, Jun,Wu, Songquan,Yang, Deok-Chun,Li, Donghao,Quan, Lin-Hu The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.4

Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at $37^{\circ}C$ for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LC-MS/MS. Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LC-MS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.

Biotransformation of Panax ginseng extract by rat intestinal microflora

Wei-Wei Dong,Jinhua Zhao,Fei-Liang Zhong,Wen-Jing Zhu,Jun Jiang,Songquan Wu,Deok-Chun Yang,Donghao Li,Lin-Hu Quan 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.4

Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at 37C for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LCeMS/MS. Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LCeMS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.

Bioinformatics Analysis Reveals Connection of Squamous Cell Carcinoma and Adenocarcinoma of the Lung

Fan, Wei-Dong,Zhang, Xian-Quan,Guo, Hui-Lin,Zeng, Wei-Wei,Zhang, Ni,Wan, Qian-Qian,Xie, Wen-Yao,Cao, Jin,Xu, Chang-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Squamous cell carcinoma and adenocarcinoma are the major histological types of non-small cell lung cancer. Because they differ on the basis of histopathological and clinical characteristics and their relationship with smoking, their etiologies may be different; for example, different tumor suppressor genes may be related to the genesis of each type. We used microarray data to construct three regulatory networks to identify potential genes related to lung adenocarcinoma and squamous cell carcinoma and investigated the similarity and specificity of them. In the network, some of the observed transcription factors and target genes had been previously proven to be related to lung adenocarcinoma and squamous cell carcinoma. We also found some new transcription factors and target genes related to SCC. The results demonstrated that regulatory network analysis is useful in connection analysis between lung adenocarcinoma and squamous cell carcinoma.

FBW7 Upregulation Enhances Cisplatin Cytotoxicity in Non-small Cell Lung Cancer Cells

Yu, Hao-Gang,Wei, Wei,Xia, Li-Hong,Han, Wei-Li,Zhao, Peng,Wu, Sheng-Jun,Li, Wei-Dong,Chen, Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Introduction: Lung cancer is extremely harmful to human health and has one of the highest worldwide incidences of all malignant tumors. Approximately 80% of lung cancers are classified as non-small cell lung cancers (NSCLCs). Cisplatin-based multidrug chemotherapy regimen is standard for such lesions, but drug resistance is an increasing problem. F-box/WD repeat-containing protein 7 (FBW7) is a member of the F-box protein family that regulates cell cycle progression, and cell growth and differentiation. FBW7 also functions as a tumor suppressor. Methods: We used cell viability assays, Western blotting, and immunofluorescence combined with siRNA interference or plasmid transfection to investigate the underlying mechanism of cisplatin resistance in NSCLC cells. Results: We found that FBW7 upregulation significantly increased cisplatin chemosensitivity and that cells expressing low levels of FBW7, such as NCI-H1299 cells, have a mesenchymal phenotype. Furthermore, siRNA-mediated silencing or plasmid-mediated upregulation of FBW7 resulted in altered epithelial-mesenchymal transition (EMT) patterns in NSCLC cells. These data support a role for FBW7 in regulating the EMT in NSCLC cells. Conclusion: FBW7 is a potential drug target for combating drug resistance and regulating the EMT in NSCLC cells.

Inhibition of cancer cell growth and migration by dihydroxynaphthyl aryl ketones

Wei Xiong,Yun-Feng Li,Shan Liu,Ting Chen,Hong-Tao Zhang,Zhi-Bin Yang,Ying-Ying Ding,De-Pei Gao,Guan-Shun Wang,Jian Dong,Jian Dong 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.4

Dihydroxynaphthyl aryl ketones 1-5 exhibit activity as tubulin polymerization inhibitors by targeting the colchicine binding site of microtubules making them potential anticancer drugs. Therefore, analogues 1-5 have been evaluated for their cytotoxic activity against the cancer cell lines DU-145 (prostate), T24 (bladder) and MCF-7 (breast). notable differences in biological activity were observed for compounds 1-5, most likely related to the nature of the aryl substituent bonded to the carbonyl group. among the tested compounds, only compound 5 showed selectivity for cancer cells over healthy, non-transformed cells. T24 cancer cells treated with compound 5 presented a concentration-dependent decrease in cell proliferation and a loss of migration ability. The cytotoxicity of compounds 1-5 on the selected cell-based assays is discussed in terms of it lipophilicity and polarizability parameters.

Conflicts Rules of Arbitration Agreements in China: Evolution and Reform

( Wei Dong Zhu ) 고려대학교 법학연구원 2012 The Asian Business Lawyer Vol.9 No.-

The Evolution of the conflicts rules of arbitration agreements in China can be divided into three stages: Before the "Interpretation 2006" was issued by the Supreme People`s Court, when designating the applicable law of the arbitration agreement the Chinese courts followed the judicial practices developed for a long time; the "Interpretation 2006" provides clear guidelines for the courts to assign the law applicable to the arbitration agreement, but the rigidity of the conflicts rules in the "Interpretation 2006" often results in the arbitration agreements being held invalid because in most if not all the cases the Chinese law will be determined as the lex causae of the arbitration agreements, greatly frustrating the parties` expectation to have their disputes settled through arbitration; the newly promulgated Law on the Application of Laws to the Foreign-Related Civil Matters (CPIL) contains a set of specific conflict rules of arbitration agreement, but such a provision is no but the old wine in the new bottle, causing more uncertainty and even contradictory results. The author analyzes the main approaches adopted in other jurisdictions in determining the validity of arbitration agreements and proposes the possible reform in Arbitration Law in China.

Adenocarcinoma of Meibomian Glands and Moll Glands in the Eyelid of Canine

Dong-wei Yuan(위엔동웨이),Moon-Jung Goo(구문정),Hai-jie Yang(양해걸),Il-Hwa Hong(홍일화),Mi-Ran Ki(기미란),Jin-Kyu Park(박진규),Hye-Rim Lee(이혜림),Sung-Eun Yoo(유성은),Kyu-Shik Jeong(정규식) 한국생명과학회 2008 생명과학회지 Vol.18 No.3

본 증례는 4년령의 암컷 비글종에서 발생한 것으로 점진적인 안구돌출증을 비롯하여 왼쪽 하안구 부속기에 발생한 직경 1.4 cm의 종양을 가지고 있었다. 종양의 병리조직학적 검경에서 증생된 종양 세포들이 소엽 및 섬유성 격벽으로 구분되는 관통상의 구조를 나타내고 있었다. 잘 분화된 피지선은 다양한 크기의 소엽을 형성하고 있으며 비만세포 및 단핵 염증세포의 침윤이 관찰되었다. 관강 내에는 이러한 관성구조의 정점부위에서 탈락되어 나온 호염성 물질이 관찰되었으며 일부 관성구조는 경도의 화생을 나타내고 있었다. 조직면역염색에서 세포집단은 α-SMA와 vimentin에 양성 반응을 나타내었다. 따라서 본 원발성 종양은 안검에서 발생한 내검판선 및 외검판선 유래의 선암종으로 진단되었으며 침윤되고 있는 안구내 종양은 악성 혼합종으로 진단되었다. A 4-year-old female beagle with progressive exophthalmos and which had a neoplastic mass with diameter of 1.4 cm in the left lower ocular adnexa. Histologically, the mass was composed of hyperplastic lobules and tubular structures separated by fibrous septum. The well differentiated sebaceous gland forming various sized lobules, and infiltration of mast cells and mononuclear inflammatory cells were observed. Apical decapitation secretion of these tubular structures with basophilic materials in their lumen showed mild sebaceous gland metaplasia. Immunohistochemical studying, cell groups were positive in α-SMA and vimentin. The primary tumor was diagnosed as adenocarcinoma originated from moll gland and meibomian gland of the eyelid, and the infiltrating intraocular neoplasm was diagnosed as a malignant mixed tumor.