The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora

Dong Xinyuan,Si Jiali,Zhang Guanghui,Shen Zhen,Zhang Li,Sheng Kangliang,Wang Jingmin,Kong Xiaowei,Zha Xiangdong,Wang Yongzhong 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Δg511). Next, the biological characteristics of the Δg511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_ s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1–β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Δg511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.

Experimental and numerical validation of guided wave based on time-reversal for evaluating grouting defects of multi-interface sleeve

Dong-Sheng Li,Jiahe Liu,Li Tang,Wei Shen 국제구조공학회 2024 Smart Structures and Systems, An International Jou Vol.33 No.1

Grouting sleeves are an essential connecting component of prefabricated components, and the quality of grouting has a significant influence on structural integrity and seismic performance. The embedded grouting sleeve (EGS)'s grouting defects are highly undetectable and random, and no effective monitoring method exists. This paper proposes an ultrasonic guided wave method and provides a set of guidelines for selecting the optimal frequency and suitable period for the EGS. The optimal frequency was determined by considering the group velocity, wave structure, and wave attenuation of the selected mode. Guided waves are prone to multi-modality, modal conversion, energy leakage, and dispersion in the EGS, which is a multi-layer structure. Therefore, a time-reversal (TR)-based multi-mode focusing and dispersion automatic compensation technology is introduced to eliminate the multi-mode phase difference in the EGS. First, the influence of defects on guided waves is analyzed according to the TR coefficient. Second, two major types of damage indicators, namely, the time domain and the wavelet packet energy, are constructed according to the influence method. The constructed wavelet packet energy indicator is more sensitive to the changes of defecting than the conventional time-domain similarity indicator. Both numerical and experimental results show that the proposed method is feasible and beneficial for the detection and quantitative estimation of the grouting defects of the EGS.

Long-term surgical outcomes of liver resection for hepatocellular carcinoma in patients with hepatitis B virus and hepatitis C virus co-infection: A multicenter observational study

Hang-Dong JIA,Lei LIANG,Chao LI,Han WU,Hong WANG,Ying-Jian LIANG,Ya-Hao ZHOU,Wei-Min GU,Xin-Ping FAN,Wan Yee LAU,Timothy M. PAWLIK,Yong-Kang DIAO,Qiu-Ran XU,Feng SHEN,Cheng-Wu ZHANG,Dong-Sheng HUANG,T 한국간담췌외과학회 2021 Annals of hepato-biliary-pancreatic surgery Vol.25 No.-

A New Furostanol Saponin from Asparagus cochinchinensis

Yang Shen,Hai-Sheng Chen,Cong-Li Xu,Wei-Dong Xuan,Hui-Liang Li,Run-Hui Liu,Xi-Ke Xu 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.10

A new furostanol saponin, (25S)-26-O-β-D-glucopyranosyl-5β-furost-20(22)-en-3β, 15β,26-triol-3-O-[α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside, namely, aspacochioside D (1) were isolated from Asparagus cochinchinensis (Lour.) Merr, along with three known saponins, aspacochioside C (2), (25S)-5β-spirostan-3β-yl-O-[O-α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside (3), and pseudoprotoneodioscin (4). The structure of 1 was elucidated on the basis of chemical reactions and spectral analysis (IR, GC, ESI-MS, ^1H-NMR, ^13C-NMR, DEPT, HMBC, HMQC and NOESY). The antiproliferative effects of 1-4 were evaluated in a cytotoxicity assay against the human tumor cell line, A549. Compound 2 (Aspacochioside C) exhibited moderate cytotoxicity against A-549, with an IC_50 value of 3.87 μg/mL.

Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells

Liao, Xian-Dong,Zhou, Chang-Hai,Zhang, Jing,Shen, Jing-Lin,Wang, Ya-Jing,Jin, Yong-Cheng,Li, Sheng-Li Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. Methods: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively. Results: In MAC-T cells, ATRA increased the mRNA levels of α_S1-casein and β-casein, janus kinase 2 (JAK2) and E74-like factor 5 of the signal transducer and activator of transcription 5 β (STAT5-β) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-β and S6K1 proteins. Additionally, ATRA increased the de novo synthesis of fatty acids, reduced the content of long-chain fatty acids, the ratio of monounsaturated fatty acids to saturated fatty acids (SFA), the ratio of polyunsaturated fatty acids (PUFA) to SFA, and the ratio of ω-6 to ω-3 PUFA. The mRNA levels of acetyl-CoA carboxylase 1, fatty acid synthase, lipoprotein lipase, stearoyl-CoA desaturase, peroxisome proliferator-activated receptor gamma, and sterol regulatory element-binding protein 1 (SREBP1) were enhanced by ATRA. Conclusion: ATRA promotes the synthesis of casein by regulating JAK2/STAT5 pathway and downstream mTOR signaling pathway, and it improves the fatty acid composition of MAC-T cells by regulating SREBP1-related genes.

李白生卒年硏究中的問題分析

楊?生(Yang Xu-Sheng),沈曙東(Shen Shu-Dong) 동아인문학회 2010 동아인문학 Vol.17 No.-

한 작기의 생몰년에 대한 연구는 틀림없이 작기에 대한 연구에서 중요한 내용이다. 특히 고대작가에 대한 연구는 더욱 그러하다. 그렇기 때문에 이백이 생몰년 문제는 그 시대 이백에 대한 연구에서 가장 주목을 받는 문제로 되어 많은 “새로운 주장”들이 나오게 되었다. 두말할 나위 없이 이런 주제에 문장을 더 뛰어나게 하려고 공을 들이는 “백가쟁명”은 이백의 생몰년에 대한 심도 깊은 연구에 유익한 점을 가져다 줄 것임은 이다. 그러나 만약 한 문제에 대한 연구가 특히 생몰년과 같은 유일한 결론이 나는 문제일 경우에는 일치된 결론을 얻을 수 없는 경지에 빠지게 되는데 이것은 학술연구자들이 바라는 바는 아니다. 그리하여 이백의 생몰년에 대한 결론이 사실에 좀 더 부합되게 하기 위해서는 “새로운 주장”에 나타나는 문제에 대하여 다시 한 번 정리해볼 필요가 있다고 생각된다. 생몰년에 대한 확정은 작가(역사인물) 연구에 있어서 중요한 일이다. 하물며 이백의 생몰년은 더욱 그러하다. 우리는 이백의 생몰년 연구에서 나타나는 문제에 대하여 순서대로 정리를 하였다. 실제로 이백의 생몰년의 연구와 확정에 도움이 될 것으로 사료된다.

Two New Acorane Sesquiterpenes from Illicium henryi

Tai-Fa Song,Wei-Dong Zhang,Xin-Hua Xia,Yun-Heng Shen,Chun-Mei Liu,Sheng Lin,Hui-zi Jin,Hui-Liang Li 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9

Two new acorane sesquiterpenes, 10-hydroxyacoronene (1) and 1β-isopropyl-4β-methyl-9β-hydroxy spiro[4.5]dec-6-en-8-one (2), one new natural product, 4-hydroxy-4, 6-dimethyl-1-tetralone (3), and one known acorane sesquiterpene, acoradiepoxide (4) were isolated from the twigs and leaves of Illicium henryi. The structures of the new compounds were elucidated primarily on the basis of analysis of spectroscopic data. In addition, the inhibitory effect on NO production of these compounds were tested. Compounds 1 and 4 exhibited slight inhibitory effects on NO production with IC50 values of 82.4 μg/mL and 76.5 μg/mL, respectively.

Two New Coumarin Glucosides from the Roots of Angelica apaensis and Their Anti-Platelet Aggregation Activity

Xiao, Wei-Lie,Shen, Yun-Heng,Niu, Xue-Mei,Sun, Han-Dong,Li, Sheng-Hong 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7

Two new coumarin glucosides, $11-O-{\beta}-D-glucopyranosyl$ thamnosmonin (1) and $12-O-{\beta}-D-glucopyranosyl$ gosferol (2), were isolated from the roots of Angelica apaensis. Their structures were elucidated spectroscopically. Both compounds showed weak inhibitory effects on rabbit platelet aggregation induced by PAF, AA and APD.

Gallbladder Tuberculosis: CT Findings with Histopathologic Correlation

Xiu-Fang Xu,Ri-Sheng Yu,Ling-Ling Qiu,Jian Shen,Fei Dong,Ying Chen 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.2

Objective: We wanted to describe the computed tomography (CT) fi ndings of gallbladder tuberculosis (TB) and to correlate them with pathologic fi ndings. Materials and Methods: There were seven patients (M:F = 3:4; mean age, 46.3 years; age range, 32 to 78 years) in whom gallbladder TB was eventually diagnosed. All of them underwent cross-sectional imaging with CT, a pathologic examination and a retrospective review. CT imaging evaluation was done in each case, including the fi ndings of a mass versus nodule, wall thickening (uniform or irregular) and the enhancement patterns (homogeneous or heterogeneous). Results: All the cases of gallbladder TB revealed the following three different CT fi ndings: micronodular lesion of the gallbladder wall (n = 1), a thickened wall (n = 4) and a gallbladder mass (n = 2). There were three cases of homogeneous enhancement of the lesions, including homogeneous enhancement with nodular lesion, homogeneous uniform thickness enhancement and homogeneous thickness enhancement in one case each, and these cases pathology showed tuberculous granuloma with a little caseating necrosis in one case and tuberculous granuloma with rich fi brous tissue, but little or no evident caseating necrosis in two cases. Four cases of heterogeneous enhancement of the lesions, including heterogeneous uniform-thickness enhancement in two cases, heterogeneous enhancement with a local mass lesion in one case and heterogeneous enhancement with a mass that replaced the gallbladder in one case; in these cases, pathology showed tuberculous granuloma with marked caseation or liquefaction necrosis in three cases and tuberculous granuloma by fi brous and calcifi cations accompanied by caseating necrosis in one case. Among the seven cases of gallbladder TB, six cases were accompanied by abdominal extra-gallbladder TB, including abdominal lymph node TB in fi ve cases and hepatic TB in four cases. Conclusion: Gallbladder TB has various CT manifestations, and the enhanced CT findings are well matched with pathological features. An irregularly thickened gallbladder wall or a gallbladder wall mass with multiple-focus necrosis or calcifi cations accompanied by the typical CT fi ndings of abdominal extra-gallbladder TB should suggest the diagnosis of gallbladder TB.

Two New Coumarin Glucosides from the Roots of Angelica apaensis and Their Anti-Platelet Aggregation Activity

Wei-Lie Xiao,Sheng-Hong Li,Yun-Heng Shen,Xue-Mei Niu,Han-Dong Sun 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7

Two new coumarin glucosides, 11-O-β-D-glucopyranosyl thamnosmonin (1) and 12-O-β-D-glucopyranosyl gosferol (2), were isolated from the roots of Angelica apaensis. Their structures were elucidated spectroscopically. Both compounds showed weak inhibitory effects on rabbit platelet aggregation induced by PAF, AA and APD.