Berberinephenolbetaine 誘導體合成 및 活性硏究[Ⅲ]

정경옥,김동현,정세영,노영수,김신규 慶熙大學校 1990 論文集 Vol.19 No.-

Nucleophilic additon of allyl magnesium bromide to berberine chloride resulted in the 8-allyl-9, 10-dimethoxy-2, 3-methylenedioxy-13, 13a-dehydroberbine[Ⅰ]. And oxidation of [Ⅰ] with m-chloroperbenzoic acid in a stream of Argon at-20∼-30℃ afforded the 8-allylberberinephenolbetaine [Ⅱ], and cycloaddition of [Ⅱ] with dialkylacetylenedicarboxylate(R; a=CH_3, b=C_2H_5) in THF gave 8-allyl-9, 10-dimethoxy-2, 3-ethylendioxy-13-oxo-8, 14-ethenodimethyldicarboxyberbine[Ⅲ a] and 8-allyl-9, 10-dimethoxy-2, 3-methylenedioxy-13-oxo-8, 14-ethenodiethyl-dicarboxy-berbine[Ⅲb]. Heating of 8, 14-ethenoberbines[Ⅲa and Ⅲb] in ethanol effected smooth isomerization leading to the 3, 4-[benzo(3",4"-dimethoxy)]-1, 8-[dihydre(2',3'-methylenedioxy) naphthalene]-5-allyl-6, 7-dimethylcarboxy-2-oxo-azocine[Ⅳa] and 3, 4-[benzo(3", 4"-dimethoxy)]-1, 8-dihydro(2', 3'-methylenedioxy)naphthalene]-5-allyl-6, 7-diethylcarboxy-2-oxo-azocine[Ⅳb]. Irradiation of berberine chloride in methanol containing rose bengal and sodium methoxide with O_2 stream afforded the 8, 9, 10, 14-tetramethoxy-2, 3-methylenedioxy-13-oxoberberine[Ⅳ], and recrystallization of [Ⅳ] from methanol gave 8-methoxyberberinephenolbetaine[Ⅶ]. And cycloadditon of [Ⅶ] with dimethyl-acetylenedicarboxylate gave 8, 9, 10-trimethoxy-2, 3-methylene-dioxy-13-oxo-8, 14-ethenodimethyldicarboxyberbine[Ⅷ], and heating of [Ⅷ] in ethanol effected smooth isomerization leading to 3, 4-[benzo(3" 4"-dimethoxy)]-1, 8-[dihydro(2',3'-methylene-dioxy) naphthalene]-5-methoxy-6, 7-dimethylcarboxy-2-oxo-azocine[Ⅸ]. The in vitro biological evaluation showed that compounds[Ⅷ] and [Ⅸ] had antibacterial effect against Pseudomonas aeruginosa, and compounds [Ⅰ], [Ⅱ], [Ⅲb] and [Ⅶ] completely had anticancer activity to P-388 mouse lymphocyle leukemia at concentration of 100㎍, and [Ⅰ] almost had anticancer activity at concentration of 20㎍.

한국인의 치아우식 조절에 관한 연구 : Ⅱ. Streptococcus mutans strain B-13이 생산한 불용성 세포외 다당류와 dextransucrase 활성 Ⅱ. INSOLUBLE EXTRACELLULAR POLYSACCHARIDES AND ACTIVITY OF DEXTRANSUCRASE PRODUCED BY STREPTOCOCCUS MUTANS STRAIN B-13, IN VITRO

정태영,정동균,이효재,김각균,오귀옥,이종흔 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Insoluble extracellular polysaccharides synthesized by Streptococcus mutans type d strain B-13 were analysed in an effort to elucidate their chemical composition. Dextransucrase produced in supernatant was also studied for its activity. 1. The microorganism produced 2.10g of total insoluble fraction which contained 1.59g of insoluble polysaccharide and 0.41g of bacterial mass per 800ml of culture medium. 2. Insoluble fraction contained 26.1㎍/mg of nitrogen and 746.4㎍/mg of insoluble extracellular polysaccharides. Ketohexose content of polysaccharides was 7.12%. 3. Streptococcus mutans strain B-13 was capable of producing 4.10g insoluble extracellular polysaccharide per 1.0g of bacterial mass. 4. Dextransucrase liberated 5.11mg of hexose per 1ml of culture supernatant.

하인두의 재발성 암종에서 대흉근피판을 이용한 하인두 재건술후 인두피하 누공 1례 : A Case Report

정성민,정옥경,이동진 梨花女子大學校 醫科大學 醫科學硏究所 1995 EMJ (Ewha medical journal) Vol.18 No.4

The treatment of the recurrent carcinoma involving hypopharynx and cervical esophagus requires radical operation and reconstruction of the food passageway. Reconstruction of the hypopharynx and cervical esophagus fo11owing extensive resection forcancer has been accomplished with various techniques. The pectoralis major myocutaneous flap remains of the greatest contributions in reconstructive head and neck surgery because it has excellent vascularity and may be raisedwithout a delay. Early studies reported either a low incidence of complications or did not describe the incidence of complications associated with the use of the pectoralis major myocutaneous flap.However, recent publications have suggested that the incidence of complications is far greaterthan previously reported. Recently, we experienced pharyngocutaneous fistula fi11owing reconstruction of the hypopharynx with the pectoralis major myocutaneous flap in recurrent hypopharynx cancer. Therefore, we analyzed the complications associated with the use of the pectoralis major myocutaneous flap for recurrent hypopharyngeal cancer after total laryngectomy with postoperativeradiotherapy.

유역 토지이용과 저수지 수질의 상관관계 분석

윤동균,정상옥 경북대학교 농업과학기술연구소 2006 慶北大農學誌 Vol.24 No.-

The objective of this study was to present regression equations between reservoir water quality and land use types of the watersheds. In order to derive regression equations, a multiple linear regression analysis was used using observed data from 88 reservoirs in the Kyungpook Provcince. The measured values of BOD, COD, T-N, and T-P were correlated with the areas of land use types. 23 regression equations were obtained for all the water quality items and watershed sizes. The results showed that 2 regression equations have the multiple correlation coefficient(MCC) above 0.90, 10 regression equations have the MCC values from 0.70 to 0.90, 9 equations have the MCC from 0.40 to 0.70, and 2 equations have the MCC from 0.20 to 0.40. The results of this study can be used to estimate reservoir water quality simply and quickly in the planning phase.

한국인의 치아우식 조절에 관한 연구 : Ⅱ.흰쥐 치아우식 이환율에 미치는 수종 양념의 영향에 관한 추가연구 Ⅱ. ADDITIONAL STUDIES ON THE EFFECTS OF THE SEVERAL SPICES ON THE CARIES RATE IN RATS

정동균,이종흔,김각균,이공훈,오귀옥,정태영 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1

Those studies were performed to elucidate the effects of several dietary spices on dental caries and to determine which component of spices induced the change of incidence of dental caries in Sprague-Dawley rats which were supplied from SNU animal house. For this study, 132 rats (rats weighing 90-140 gm) were chosen at random with the same number of both sexes and divided into eleven experimental groups which contained the differential components of drinking water and diets for control and experimental group. Control group was fed standard cariogenic diet and tap water. In experimental groups, rats were fed standard cariogenic diet supplemented with capsicum (10%), ash capsicum (1, 10%), garlic (5%), common salt (3%), and analytical salt (3%) for 80 days. Drinking water was tap water containing water extract of capsicum (1, 10%), water extract of garlic (1.1%), and fluoride (50ppm). The results were as follows. 1. 54.6 in caries score was recorded in control group. 2. Rats which drank water containing 50ppm of fluoride showed 18.4% decrease in caries score. 3. Capsicum-fed rats showed 10.7% (capsicum 10%) increase in caries score and rats which drank water containing water extract of capsicum (1, 10%) showed 14.1% (w.e. capsicum 1%) and 0.16% (w.e. capsicum 10%) increase in caries score. But ash capsicum-fed rats showed 18.7% (ash capsicum 1%) and 7.3% (ash capsicum 10%) decrease in caries score. 4. Garlic-fed rats showed 22.1% (garlic 5%) decrease in caries score. But rats which drank water containing 1.1% of water extract of garlic showed 24.1% increase in caries score. 5. 58.4% and 68.0% increase in caries score were recorded in rats which were fed diet containing common salt (3%) and analytical NaCl (3%), respectively.

한국인의 치아우식 조절에 관한 연구 : Ⅲ. 白鼠우치痍患率에 미치는 數種 稀 有元素, 쌀 및 各種 양념의 影響 Ⅲ.EFFECTS OF SEVERAL TRACE ELEMENTS, RICE AND SPICES ON THE CARIES RATE IN RATS

鄭東均,鄭素英,李孝宰,金各均,吳貴玉,李種昕 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

Two-part experiment was conducted to elucidate the effects of several trace elements, rice and spices on caries rate in Korean albino rats. Rats were selected into two large groups according to their weight ; one group (for part I experiment) consists of rats weighing 140-200gm and the other group (for part II experiment) consists of rats weighing 76-109gm, and the same experiment was conducted independently for both groups. Rats were fed standard cariogenic diet supplemented with rice (21, 53%), capsicum (1, 10%), garlic (0.5, 5 or 10%), phosphorus (2%), sodium chloride (3%, reagent grade), or table salt. Drinking water was tap water containing cold water extract of capsicum (0.1%) alcoholic extract of capsicum (0.1%), water extract of ginseng (2%), fluoride (10, 50 or 100ppm) or 50ppm of Mg, Zn, Mo, Sr, Mn of Fe. The results were as follows. 1. 24.9%, 51.0% and 54.3% decrease in caries score were recorded for rats which drank water containing 10, 50 and 100ppm of fluoride in part I experiment. In part II experiment caries score decreases 51.8% and 56.9% for 10ppm and 50ppm fluoride content. 2. Capsicum-fed rats showed 51.7% (capsicum 1%) and 58.1% (capsicum 10%) decrease in caries score in part I experiment. 21.5% and 33.6% decrcase were recorded for part II experiment. 3. Garlic-fed rats showed 44.5% (garlic 0.5%), 23.0% (garlic 5%) and 53.7% (garlic 10%) decrease in caries score. 4. Sodium chloride-fed rats showed 122.6% (NaCl 3%) increase in caries score. 5. Rats which were fed diet containing 2% Na_2HPO_4 showed 26.7% decrease in caries score and further 41.6% decrease when 10ppm fluoride was given simultaneously. 6. 28.4% and 31.5% decrease in caries score were recorded for rats which drank water containing 50ppm of Mg and Zn.

한국인의 치아우식 조절에 관한 연구 : Ⅳ. 金堤郡 치문국교 학동의 尿中弗素濃度, 班狀齒指數 및 學童居住地域의 飮料水弗素濃度에 關한 硏究 Ⅳ. STUDY ON THE RELATION BETWEEN THE URINARY FLUORIDE CONCENTRATION, THE FLUOROSIS INDEX AND THE FLUORIDE CONCENTRATION OF DRINKING WATER IN BAIKKU-MYUN, KIMJAE-KUN

丁東均,李種昕,李孝宰,金各均,吳貴玉,鄭泰英 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1

The urinary fluoride concentration and the degree of dental fluorosis of all the schoolboys attending Chimoon primary school in Baikku-myun, Kimjae-kun and the fluoride concentration of drinking water of those areas were investigated in an effort to elucidate the relation between them. The urinary fluoride concentration and the fluoride concentration of driking water were measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1) The mean urinary fluoride concentration and the mean fluorosis index of all the schoolboys were 1.89ppm (0.12-12.2ppm) and 0.79 (0-1.99). The mean fluoride concentration of drinking water of those areas was 0.61ppm (0.027-2.66ppm). 2) The mean urinary fluoride concentration and the mean fluorosis index of the schoolboys residing in Yookang-ri 5-ku, Baikku-myun (Dongja-po) were the highest (3.23ppm, 1.99) among the statistics for the schoolboys residing in different areas. Incidentally the fluoride concentration of drinking water for that area was also the highest (1.99ppm). 3) The urinary fluoride concentration was two to ten times as high as the fluoride concentration of drinking water regardless of the resident area of schoolboys. 4) There was no significant seasonal change in fluoride concentration of drinking water collected from the well in the resident area of schoolboys. 5) There showed a relatively parallel relationship between the urinary fluoride concentration and the fluorosis index of schoolboys. 6) The urinary fluoride concentration and the fluorosis index of schoolboys tend to increase with age increasing.

草鳥중 Aconitine계 Alkaloids의 定量

嚴東玉,李來洪,宣中基,都京三 又石大學校 1995 論文集 Vol.17 No.-

This study was undertaken to develope a method for the determination of aconitine alkaloids from Aconitum ciliare Tuber by spectrophotometry. The complex of cobalt(Ⅱ)ion and thiocyanate ion forms tetrathiocyanatocobaltate(Ⅱ)ion in aqueous solution containing an excess of thiocyanate ion. The tetrathiocyanatocobaltate(Ⅱ)ion produces the complex with aconitine alkaloids. The complex are extracted from aqueous solution to 1. 2-dichlorethane. A spectrophotometry for determination of aconitine alkaloids with tetrathiocyanatocobaltate(Ⅱ)ion were, established by 1, 2-dichlorethane extraction. Determination of aconitine standard was possible to the rage of 0.4-0.6㎎/㎖ in 1.2-dichlorethane. To examine the determination of aconitine alkaloids in Aconitum ciliare Tuber were determined by using acid-alkali titration, spectrophotometry from ether extracts. The average recovery of aconitine standard sloution was 97.9% and standard deviation 2.97%. As this method is accurate and precise, is applicable to the determination of aconitine alkaloids in Aconitum ciliare Tuber.

한국인의 치아우식 조절에 관한 연구 : Ⅲ. 만경강 유역 주민의 뇨중 불소농도, 반상치 지수에 관한 연구 STUDY ON THE RELATION BETWEEN THE URINARY FLUORIDE CONCENTRATIONAND THE FLUOROSIS INDEX OF RESIDENTS IN MANKYUNG RIVER VALLEY

정동균,정태영,이종흔,김중수,오귀옥,이효재 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1

This experiment was conducted to elucidate the relation between the urinary fluoride concentration and the fluorosis index of residents in Mankyung River valley, especially in 3 areas (Yookang-ri, Koje·Yoochun, Shinbok·Yongkang) with high fluoride concentration in drinking water. The urinary fluoride concentration was measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1. There was no reletionship between the urinary fluoride concentration and age. 2. 11-20 years age groups has revealed the highest fluofosis index. In this group : 3. There showed a parallel relationship between the fluorosis index and the urinary fluoride concentration. 4. DMF rates of residents in Yookang-ri, Koje and Shinbok are 59.2, 48.4 and 47.1%, respectively. 5. There showed a parallel relationship between the years of residence within 8 years after birth and the fluorosis index.

Glow放電法에 의한 α-Si蒸着裝置의 製作과 그 試料의 特性

유동선,현옥배,고년규,황정남,정원모,이철주 연세대학교 자연과학연구소 1981 學術論文集 Vol.7 No.-

Glow방전법에 의해서 SiH_4를 분해하여 α-Si 박막을 얻을수 있는 장치를 제작하였다. 이 SiH_4기체는 수소기체속에서 10%로 희석되였으며, 진공방전함속에서 rf 전압에 의하여 분해된 Si는 유리기판위에 증착된다. α-Si박막은 1~1.6 A˚/sec의 증착율로 약 7000 A˚의 두께까지 증착되였다. α-Si 박막의 흡수계수는, 2.3 eV의 photon energy 영역에서 약 10^4 cm^-1이고, 그 optical gap은 약 2.1 eV였다. 이 박막의 증착율은 전극간격에 따라 변하고 있으나, 최적전극간격과 기체유입량은 각각 2.6cm와 1~2cc/min였다. Apparatus capable of producing α-Si film by the glow discharge method was designed and constructed in this study. The monosilane gas which is diluted in H_2 gas to 10% is decomposed in the reaction chamber by rf oscillation. The apparatus is composed of a reaction chamber, a rf generator, a cooling system, etc. The α-Si thin film is produced at a deposition rate of 1~1.6 A˚/sec to a thickness of about 7000 A˚. Its absorption coefficient is about 10^4 cm^-1 in the photon energy region of ~2.3 eV, and its optical gap is about 2.1 eV. The deposition rate for the film is controlled by varying the distance between electrode and substrate. The optimum distance and gas flow is about 2.6 cm and 1~2 cc/min, respectively.