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1
Review : Advances in Rapid Detection Methods for Foodborne Pathogens

( Xi Hong Zhao ),( Chii Wann Lin ),( Jun Wang ),( Deog Hwan Oh ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3
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  ScienceON
  
  KCI
  
  KISS
Food safety is increasingly becoming an important public health issue, as foodborne diseases present a widespread and growing public health problem in both developed and developing countries. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent human foodborne infections. Traditional microbiological detection and identification methods for foodborne pathogens are well known to be time consuming and laborious as they are increasingly being perceived as insufficient to meet the demands of rapid food testing. Recently, various kinds of rapid detection, identification, and monitoring methods have been developed for foodborne pathogens, including nucleic-acid-based methods, immunological methods, and biosensor-based methods, etc. This article reviews the principles, characteristics, and applications of recent rapid detection methods for foodborne pathogens.
2
An electromagnetically-driven microfluidic platform with indirect-heating thermo-pneumatic valves

Bonnie T.,Xin-Ying Yang,Ming-Yuan Cheng,Chii-Wann Lin,Yao-Joe Yang 한국바이오칩학회 2011 BioChip Journal Vol.5 No.2
In this work, we present a microfluidic platform which is capable of carrying out a series of laboratorial operations on a disposable chip. The platform employs coil arrays to transport biological samples attached on magnetic beads through different reaction zones in aqueous solutions. Micromachined thermopneumatic valves are adopted as the interfaces between sequential reactions. The self-contained system is composed of a disposable microfluidic reaction (MFR) chip and a fluidic driving/sensing (FDS) module. Various reaction zones with different functionalities are implemented on the MFR chip. Arrays of coils and micromachined heater/sensing chips are integrated into the FDS module. Also, novel indirect-heating thermo-pneumatic valves (IH-TPVs), which are monolithically integrated into the disposable MFR chip, are proposed and characterized. With an applied voltage of 3.2V, the IH-TPV effectively isolates two adjacent reaction zones, while its temperature elevation is sufficiently low for biological samples and reagents. By employing the proposed approach, we design and implement a miniaturized biochip system which carries out the processes of DNA extraction, purification and amplification from whole blood sample. The dimension of the fully-integrated system is 72 mm×54 mm×11 mm, and the system could be fully operated with a 12 V DC power supply. DNA extraction and purification from human whole blood followed by PCR amplification for the 122 bp segment is successfully performed by using the system.