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Microbial Production of Fatty Acid via Metabolic Engineering and Synthetic Biology
Chandran Sathesh-Prabu,Kwang Soo Shin,Geun Hwa Kwak,Sang-Kyu Jung,Sung Kuk Lee 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.1
The microbial production of free fatty acids (FFAs) has gained great attention from the scientific community due to its significant environmental and economic benefits. FFAs can also be used as precursors for the production of valuable products, polymer additives and industrial chemicals by various biological or chemical reactions. Since microorganisms synthesize fatty acids (FAs) which are mainly used to form lipids for cell membrane constitution, FFAs are not normally accumulated as metabolic intermediates. FA metabolism is tightly regulated at transcriptional and post-transcriptional levels by both the transcription factor and product inhibition, meaning that FA overproduction may require extensive re-engineering of cellular metabolism. Therefore, we here present the recent efforts applied to enhance FFA production via metabolic engineering and synthetic biology with special reference to Escherichia coli.
Mutation Breeding of Mushroom by Radiation
Chandran Sathesh-Prabu,이영근 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.4
Mushrooms belonging to macrofungi have been consumed by humans for their nutritionaland medicinal values for centuries throughout the world. Mushroom farming is practiced in morethan 100 countries of the world, with production increasing at a rate of 7% per annum. High yieldand good quality are always the principal goals for agriculturally important crops, including mushrooms. Several breeding methods are employed for strain improvement such as mass selection basedon the natural chance mutation and induced mutation (mutation breeding), protoplast fusion technology,cross breeding and transgenic breeding. However, mutation breeding has shown prominentsuccess in crop plant improvement. Though several-hundred mutant crop varieties have beendeveloped around the world, the mutation breeding of mushrooms is limited. This review paperexplores the potential application of radiation on the development of mutant varieties of mushroomsfor breeding with desired traits such as better quality and productivity.
Sathesh-Prabu, Chandran,Lee, Sung Kuk American Chemical Society 2015 Journal of agricultural and food chemistry Vol.63 No.37
<P>Long-chain α,ω-dicarboxylic acids (LDCAs, ≥C12) are widely used as a raw material for preparing various commodities and polymers. In this study, a CYP450-monooxygenase-mediated ω-oxidation pathway system with high ω-regioselectivity was heterologously expressed in <I>Escherichia coli</I> to produce DCAs from fatty acids. The resulting engineered <I>E. coli</I> produced a maximum of 41 mg/L of C12 DCA and 163 mg/L of C14 DCA from fatty acids (1 g/L), following 20 h of whole cell biotransformation. Addition of a heme precursor and the hydroxyl radical scavenger, thiourea, increased product concentration (159 mg/L of C12 DCA and 410 mg/L of C14 DCA) in a shorter culture duration than that of the corresponding controls. DCAs of various chain lengths were synthesized from coconut oil hydrolysate using the engineered <I>E. coli</I>. This novel synthetic biocatalytic system could be applied to produce high value DCAs in a cost-effective manner from renewable plant oils.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2015/jafcau.2015.63.issue-37/acs.jafc.5b03833/production/images/medium/jf-2015-03833u_0008.gif'></P>
Carbon Source- or Substrate- Inducible Promoter Systems for Pseudomonas putida KT2440
Sathesh-Prabu CHANDRAN,Sung Kuk LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The production of biobased bulk chemicals from renewable bioresources could minimize the negative impacts of conventional chemical-based productions on the environment and the challenges posed by depleting resources of natural petrochemicals. Pseudomonas putida strain KT2440 is a prominent metabolic engineering and synthetic biology chassis for industrial and medical applications because of its robustness and metabolic versatility. Inducible and tuneable expression systems are essential for microbial production of biochemicals. Here, carbon source- or substrate- inducible promoter systems for P. putida KT2440 strain were developed. The systems can be induced by the low-cost substrates such as glucose, xylose, levulinic acid and 3-hydroxypropionic acid. These substrates can be used as the potent starting material for both cell growth and production of a wide range of biochemicals. The systems are tuneable and controllable. LvaR/PlvaA and XutR/PxutA systems were sensitive even at low concentration of levulinic acid (0.1 mM) and xylose (0.5 mM), respectively. As the efficiency of the reported systems were very much comparable with the conventional chemical inducible systems, their limitations such as high cost, cell toxicity and difficulties in downstream process could be avoided. Hence, the newly investigated promoter systems are highly useful for the expression of the target genes in the widely used synthetic biology chassis Pseudomonas putida KT2440 for industrial and medical applications.