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- 저자 : ( Am Jang ) (검색결과 276 건)
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김선민,이태용,오장균,박암 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.1
The author studied the recognition of patient's right and the degree of one's satisfaction to help set the index of doctor-patient relationship. The data was collected from 423 patients who were admitted to the hospital in Taejeon for fifteen days from Aug. 10, 1991 to Aug. 25, 1991. The results were as the follows: 1. The recognition of the patient's right and the degree of satisfaction were 4.46±0.734, 3.20±0.884 of total 5.0 score in each mean level. 2. "I believe a patient has a right to be treated equal.", "I believe a patient has a right to be compensated in medical accident." and "A patient should be told his diagnosis, treatment, prognosis about the disease by the doctor." were high mean level among each question. Their mean levels were 4.73, 4.69, 4.66 in sequence. 3. The recognition of patient's right by the age was the highest in teenager. It was lowest in above fifty years old. It was lower as age increase. There were statistically significant difference(P<0.05). The recognition of patient's right by the level of education was highest in college level and lowest in primary school level, but the degree of satisfaction was highest in primary school level and lowest in high school. The higher the education level was, the higher the recognition of patient's right was, but the lower it was, the higher the degree of satisfaction was. There was statistically significant difference(P<0.01). The recognition of patient's right be family income was highest in high income level but the degree of satisfaction was lowest. There was statistically significant difference in the recognition of patient's right(P<0.01). 4. Correlation between the recognition of patient's right and the degree of its satisfaction: "To be advised if the hospital proposes to engage in human experimentation affecting his care to patient one has the right to refuse to participate in such research projects was positive correlation with the degree of its satisfaction(r=0.1153). There was statistically significant difference(P<0.01). There were no correlation in other questions. 5. Correlation among each questions for the recognition of patient's right was highest between "…to expect that all communications and records pertaining to his care should be treated as confidential" and "…the right to know what hospital rules and regulations apply to his conduct as a patient"(r=.6314). 6. Correlation among each questions for the degree of satisfaction was highest between "…to considerate and respectful care" and "…to expect that within its capacity a hospital must make reasonable response to the request of a patient for services"(r=.6314).
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Jang, Seong Han,Jang, Ho Am,Lee, Junbeom,Kim, Jong Uk,Lee, Seung Ah,Park, Kyoung-Eun,Kim, Byung Hyun,Jo, Yong Hun,Lee, Bok Luel American Society for Microbiology 2017 Applied and environmental microbiology Vol.83 No.11
<P>IMPORTANCE Bacterial polyhydroxyalkanoate (PHA) biosynthesis is a complex process requiring several enzymes. The biological roles of PHA granule synthesis enzymes and the surface proteins of PHA granules during host-gut symbiont interactions are not fully understood. Here, we report the effects on colonization ability in the host midguts and the fitness of host insects after feeding Burkholderia mutant cells (four phaP-depleted mutants and one phaR-depleted mutant) to the host insects. Analyses of both synthesized PHA granule amounts and CFU numbers suggest that the phaR gene is closely related to synthesis of the PHA granule and the colonization of the Burkholderia gut symbiont in the host insect's midgut. Like our previous report, this study also supports the idea that the environment of the host midgut may not be favorable to symbiotic Burkholderia cells and that PHA granules may be required to adapt in the host midgut.</P>
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( Am Jang ),( In Soo Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.3
The ability to detect and enumerate the specific bacteria is essential for determining their fate in various biological processes. Although fluorescent in situ hybridization (FISH) technique using rRNA-targeted oligonucleotide probes may be considered as the most rapid method for identification of a single bacterial cell, it could not efficiently count cells due to the limitations of light microscopy. In this study, to make accurate estimates of the active nitrifying bacterial populations from activated sludge samples, applied FISH method using Luminescence Spectrometer LS50B was used. Enumeration of nitrifying bacteria by FISH using Luminescence Spectrometer can help in the optimization of the performance of biological nitrogen removal processes.
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Jang Ho Am,Park Ki Beom,Kim Bo Bae,Ali Mohammadie Kojour Maryam,Bae Young Min,Baliarsingh Snigdha,Lee Yong Seok,Han Yeon Soo,Jo Yong Hun 한국곤충학회 2020 Entomological Research Vol.50 No.12
Defensins are a major family of antimicrobial peptides that serve as the innate immune defense of both vertebrates and invertebrates. Due to their antimicrobial, chemotactic, and regulatory activities, Defensins have been exploited for their therapeutic potential. Insect Defensins are cysteine‐rich and contain an N‐terminal loop, α‐helix, and antiparallel β‐sheet, forming a “cysteine‐stabilized alpha beta (CSαβ)” or “loop–helix‐sheet” structure. In this study, we identified the full‐length open reading frame (ORF) sequences of Defensin (TmDef) and Defensin‐like (TmDef‐like) genes from the mealworm beetle Tenebrio molitor using in silico methods. TmDef and TmDef‐like genes encode the peptides of 72 and 71 amino acid residues, respectively. TmDefensin is comprised of a Defensin domain and the TmDefensin‐like is comprised of a signal peptide of 21 amino acid residues. Phylogenetic analysis revealed close similarities of TmDefensin with the Defensin of Acalolepta luxuriosa of the longhorn beetle family. The expression of TmDef mRNA was found to be greater than that of TmDef‐like mRNA and was mostly expressed in the pupal and adult stages. Tissue distribution showed high expression of TmDef‐like mRNA in larval hemocytes, gut, integument, and fat body, while in adults, the expression was high in gut and hemocytes. Following bacterial and fungal stimulation in vivo, TmDef was upregulated at 24 h post‐infection in whole body, fat body, and hemocytes of the larvae. Even TmDef‐like mRNA was upregulated in the gut and hemocytes at 12 and 9 h post‐infection respectively. These results suggest that TmDef and TmDef‐like genes play important roles in protecting T. molitor from microbial contact.
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Jang Ho Am,Park Ki Beom,Kim Bo Bae,Ali Mohammadie Kojour Maryam,Bae Young Min,Baliarsingh Snigdha,Lee Yong Seok,Han Yeon Soo,Jo Yong Hun 한국곤충학회 2020 Entomological Research Vol.50 No.9
Antimicrobial peptides (AMPs) are effector candidates that elicit humoral immunity in invertebrates. AMPs facilitate bacterial clearance by either physically disrupting the microbial membranes or the intracellular targets. In the Coleopteran pest, Tenebrio molitor, transcriptional regulation of AMPs has been studied in the context of innate immune signaling cascades and antimicrobial immunity. Here, we report the transcriptional response of three AMP genes, Coleoptericin A, B, and C (TmCole A, B and C) in T. molitor in response to bacterial (Escherichia coli, Staphylococcus aureus), and fungal (Candida albicans) challenges. These genes were expressed essentially in the gut and hemocytes followed by the integument tissue of the T. molitor larva. Further, these genes were highly expressed in the late-larval, pupal, and early adult stages. Furthermore, while all of these transcripts were highly upregulated in the fat body and Malpighian tubules after bacterial challenge, TmCole B and TmCole C were induced in the gut after E. coli challenge. Fungal stimulation was not required for the upregulation of the transcription of Coleoptericin genes in T. molitor.
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Jang, Ho Am,Seo, Eun Sil,Seong, Min Young,Lee, Bok Luel Elsevier 2017 Developmental and comparative immunology Vol.67 No.-
<P><B>Abstract</B></P> <P> <I>Riptortus pedestris</I>, a common pest in soybean fields, harbors a symbiont <I>Burkholderia</I> in a specialized posterior midgut region of insects. Every generation of second nymphs acquires new <I>Burkholderia</I> cells from the environment. We compared <I>in vitro</I> cultured <I>Burkholderia</I> with newly <I>in vivo</I> colonized <I>Burkholderia</I> in the host midgut using biochemical approaches. The bacterial cell envelope of <I>in vitro</I> cultured and <I>in vivo Burkholderia</I> differed in structure, as <I>in vivo</I> bacteria lacked lipopolysaccharide (LPS) O-antigen. The LPS O-antigen deficient bacteria had a reduced colonization rate in the host midgut compared with that of the wild-type <I>Burkholderia</I>. To determine why LPS O-antigen-deficient bacteria are less able to colonize the host midgut, we examined <I>in vitro</I> survival rates of three LPS O-antigen-deficient <I>Burkholderia</I> mutants and lysates of five different midgut regions. The LPS O-antigen-deficient mutants were highly susceptible when cultured with the lysate of a specific first midgut region (M1), indicating that the M1 lysate contains unidentified substance(s) capable of killing LPS O-antigen-deficient mutants. We identified a 17 kDa protein from the M1 lysate, which was enriched in the active fractions. The N-terminal sequence of the protein was determined to be a soybean Kunitz-type trypsin inhibitor. These data suggest that the 17 kDa protein, which was originated from a main soybean source of the <I>R. pedestris</I> host, has antibacterial activity against the LPS O-antigen deficient (rough-type) <I>Burkholderia.</I> </P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Burkholderia</I> LPS O-antigen-deficient bacteria had a reduced colonization rate in the host midgut. </LI> <LI> <I>In vitro</I> survival rates of three LPS O-antigen-deficient mutants were examined. </LI> <LI> A specific midgut contains substance killing LPS O-antigen-deficient mutants. </LI> <LI> 17 kDa trypsin inhibitor has antibacterial activity against the LPS O-antigen-deficient mutant. </LI> </UL> </P>
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In silico identification and expression analysis of superoxide dismutases in Tenebrio molitor
Jang Ho Am,Shin Hyeon Jun,Lee Seo Jin,Kim Jae Hui,Kim Jae Hui,Kang Dong Woo,Choi So Yeon,Jung Sang Mok,Shin Hyun Woung,Lee Yong Seok,Han Yeon Soo,Jo Yong Hun 한국유전학회 2024 Genes & Genomics Vol.46 No.7
Background Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage. Objective To investigate expressions of SODs under oxidative stress in Tenebrio molitor. Methods Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR. Results Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu2+ binding site, Zn2+ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs. Conclusion Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor. Background Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage. Objective To investigate expressions of SODs under oxidative stress in Tenebrio molitor. Methods Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR. Results Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu2+ binding site, Zn2+ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs. Conclusion Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor.
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