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RISS 인기검색어
- 검색키워드
- 발행기관 : Gachon Univ. (검색결과 18 건)
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Impedance based cell culture platform for nanotechnology based drug formulations
Bennet, Devasier Gachon Univ. 2013 국내박사
Advancement in bio-nanotechnology has expanded our ability to design new regeneration applications in the field of photo-oxidative therapeutics. Light incidence morbidity and mortality of human eye and skin diseases have continued to rise. Continuous exposure causes photo-oxidative damages, degenerative disorders including retinopathies retinitis pigmentosa, computer vision syndrome, premature aging, and skin diseases and disorders. All together the radiation-induced photo-oxidative mechanism has mediated by reactive oxygen species (ROS). Surplus production of free radicals activates various diseases including vascular dysfunction and damage to cellular macromolecules and nucleic acid. Targeting ROS is therefore an important strategy for protection. While the early stages of the disease can often be cured by appropriate therapy, it will allow patients to improve their quality of life. However, the potential role of this early stage of degenerative process is neither well understood nor explored. For this reason the treatment of light toxicity remains challenge in optometry and dermatological intervention. In other side continuous monitoring cellular responce is very difficult with existing methods. Understanding their importance is a prerequisite to develop more sensitive and real-time analytical tools to rectify existing drawbacks. The objective of this thesis work is to development of analytical tool and newer approach of biomedically important targeted towards eye and skin. In our present research developed an analytical tool, which unprecedented and real-time in vitro light setup was established using a bioimpedance system to measure the cell photo-oxidative damage and protective effect of various drugs. This thesis is based on the following chapters. Chapter 1 provides the general description about research and developments for phototoxicity-oxidative damages in eye and skin cells, especially highlighting the retinal and skin damage existing in the literature. Also addressing the virtual need in research and developments for therapeutics, and exploring analytical platform. Chapter 2 is an experimental report demonstrating the cellular behavior and real-time evaluation of light-induced stress in retinal cells. Also, the different color light-induced effects on the response of cells that exposed to protective drugs were studied. Finally, the effects of agmatine and resveratrol on light-induced damage and its underlying photo-oxidative and protective mechanisms were monitored, and its helps to rescue damaged cells. Chapter 3 describes an analytical tool, which unprecedented, simple and real-time in vitro light setup was established using a bioimpedance system to measure the skin cell photo-oxidative damage and protective effect of quercetin-loaded PLGA nanoparticle. Also the phyto-medicine applications to protect against skin cell photo-damage. The continuous real-time evaluations of photo-damage and photo-protective effect of phyto-compounds on skin cells were studied. Chapter 4 deals with targeting oxidative stress with inhibiting or boosting the endogenous levels of antioxidants effect in the treatment of oxidative-stress-related diseases. It describe permeation enhanced nanoparticle for enhanced transdermal delivery. Also describes the dual drug-loaded nanoparticles incorporated swelling-induced scaffold film for controlled transdermal drug-delivery system to prevent first-pass metabolism. Chapter 5 provides the overall concluding remarks and future perspectives about the different phenotypic skin cell (human dermal fibroblast cells and human epidermal keratinocyte cells) response to light radiation and to elucidate mechanism of melanoma regeneration applications.
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Smart point-of-care systems for molecular diagnostics based on nanotechnology
Devadhasan, Jasmine Pramila Gachon Univ. 2013 국내박사
Complementary Metal-Oxide-Semiconductor (CMOS) based sensor systems continue to play an important role in various biomedical, chemical, industrial, food safety, national security and defense, and environmental applications. Among several applications, molecular diagnostics have attracted a great deal of consideration in biomedical applications. Objective of this thesis work is to develop CMOS image sensor based molecular diagnostics with a central focus towards the enhancement of point-of-care (POC) approach in whole blood glucose analysis and immunoassay. Development of chip fabrication, synthesis of ammine functionalized silica (AFSiO2) nanoparticles and various thickness of thermally evaporated indium nanoparticle (InNP) substrate preparation are the part of the research to achieve the CMOS image sensor based molecular diagnostics system. The thesis based on the following chapters. Chapter 1 provides the general introduction about CMOS image sensor and their biomedical applications, especially highlighting the CMOS image sensor based molecular diagnostics in glucose monitoring and immunoassay existing in the literature, followed by addressing the virtual need in research and developments for glucose sensor and immune sensor. Chapter 2 describes the CMOS image sensor based glucose monitoring with new approach. To achieve the goal, a series of studies were performed to establish the CMOS image sensor based whole blood glucose monitoring with POC approach. Study 1 is a preliminary investigation for developing novel optical sensor system for measurement of glucose. Experiments in aqueous glucose solution were designed to demonstrate the feasibility of the proposed technique for the glucose monitoring detected by CMOS image sensor. Study 2 demonstrates CMOS image sensor based various concentration of mouse plasma glucose monitoring. Study 3 explains fabrication of PET film chip and synthesis of AFSiO2 nanoparticles for enzyme immobilization were developed for an innovative method of whole blood glucose monitoring using CMOS image sensor as a POC approach. For the analysis, various concentration of mouse whole blood was used to accomplish the assay with real time monitoring. A series of studies were performed to establish the CMOS image sensor based molecular diagnostics in immunosensing which includes in chapter 3, study 1 explains development of InNP substrate by thermal evaporation method and the practical applicability of C-reactive protein (CRP) and troponin I antigen-antibody (Ag-Ab) interaction on various thickness of InNP substrate. Study 2 extends the unraveling the phenomena involved in CMOS image sensor based detection of CRP and troponin I Ag-Ab interaction. Study 3 explains the topographical studies of HIV Ag-Ab interaction and study tried to prove overcomes of non-specific interaction on InNP substrate. Study 4 demonstrates the morphological characterization study of HBV Ag-Ab interaction on InNP substrate and the comparative study of conventional ELISA method. This study proved that the CMOS image sensor is a potential device for immune diagnostics and could be utilized with POC applications. Chapter 4 provides overall concluding remarks and chapter 5 demonstrates the future perspectives about the CMOS image sensor molecular diagnostics the field of glucose monitoring and immunoassay by POC approach. This research will lead to the development of a small, low power, optical sensor for glycemic, cardiac and infectious disease patients, which will be a major breakthrough in the area of molecular diagnostics, upon successful completion of this research and development of the device.
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