回收 處理가 펄프纖維의 製紙特性에 미치는 影響

김형진 江原大學校 1989 국내석사

아세트酸-물 溶媒系에 의한 木材의 有機酸 蒸解 效果

이선호 江原大學校 1989 국내석사

落葉松 抽出物의 單離

최형주 江原大學校 1985 국내석사

木材의 冷凍이 木材乾燥에 미치는 效果

고재석 江原大學校 大學院 1982 국내석사

리그닌 모델 化合物의 合成

이현종 江原大學校 1989 국내석사

韓國産 闊葉樹材 穿孔板의 微細形態에 關한 硏究

김대영 강원대학교 대학원 1995 국내석사

This study was carried out to investigate the micromorphology of perforation plate that are the wall involved in the coalescence of two adjacent elements of vessel of Korea native hardwoods of 78 species, 45 genera and 25 families using SEM. Results obtained from this study are summarized as follows; 1. Perforation plates were divided into three types, simple, scalariform and multiple perforation plates. Based on the occurrence of these types within species, the species examined were classified into the following six groups: (1) 60 species (35 genera, 21 families) having exclusively simple perforations, (2) 5 species (2 genera, 1 families) having exclusively scalariform perforation plates, (3) 4 species (4 genera, 3 families) having simple and scalariform perforation plates, (4) 2 species (2 genera, 1 families) having simple and multiple perforation plates, (5) 5 species (3 genera, 3 families) having scalariform and multiple perforation plates, (6) 2 species (2 genera, 2 families) having simple, scalariform and multiple perforation plates. 2. The most of species of Betulaceae showed scalariform perforation plates, while Carpinus tschonoskii had simple perforation plates. 3. Quercus aliena of Fagaceae was observed vestured perforation that occurred around bordered rim of simple perforation plates. 4. The length of vessel elements with simple perforation plates was short and the inclination of perforation plates was slow slope. 5, Perforation rims which are observed in simple perforation plate were classified according to their occurrence and shapes into five types ', Q3 Having prominent bordered rim(Type I), 0 Having moderate bordered rim(Type II), Q3 Having non-bordered with blunt edge(Type III), (43 Having non-bordered with pointed edge(Type IV), (5) Having bordered rim with thick compound middle lamella(Showing a triple bordered rim, Type V). Among these types, Type IV was observed the most frequently, Type III showed relatively high occurrence, Type II showed maderate present and occurrence degree of Type I and V was low. Species having two kinds of perforation rims was 18species. 6. Among Betulaceae having only scalariform perforation plate, Alnus had many barred, closed and regular scalariform perforation plates, but Betula had few barred, wide and irregular scalariform perforation plates. 7. In the species with both simple and scalariform perforation plates or simple and multiple perforation plates, the occurrence rate of simple perforation plate was high. Also, in the species having scalariform and multiple perforation plate, that of scalariform perforation plate was high. 8, According to the previous report, Crataegus pinnatifida and Sorbus alnifolia had only simple perforation plate, but result of this work showed that these species had both simple and irregular multiple perforation plates, 9. Cornus kousa showed the scalariform and multiple perforation within a perforation plate. 10. The combination perforation plates were observed in 7species having more than one type of perforation plates. 11. Mismatching perforation plates were observed in 2 species among 5 species having scalariform perforation plates. 12. Substructure in opening bar of perforation plates were observed in Betula davurico and Cercidiphyllum japonicum.

Metallothionein 유전자를 이용한 카드뮴 내성 및 흡수능에 관한 연구

이은희 전남대학교 대학원 2008 국내석사

Metallothionein(MT)은 구성 아미노산의 약 1/3이 cystein 으로 이루어진 저분자량(<10 kDa)의 단백질로서, 중금속이 흡수되면 이들을 결합(chelation)시키는 역할을 수행한다. 본 실험에서는 토란(Colocassia esculenta)에서 Ce-MT2의 유전자를 처음으로 cloning하였으며, 또한 이를 유전공학적으로 변형시킨 Mod-MT2를 만들어 내었다. 이와 더불어 애기장대(Arabidopsis thaliana)에서 Ara-MT2를 cloning하였다. Ce-MT2 유전자는 다른 식물들로부터 cloning된 MT유전자들과 상동성(homology)을 비교해 본 결과 70%정도의 유사성을 보였다. 각각의 MT유전자를 E. coli에 발현시킨 후 카드뮴에 대한 내성 및 카드뮴 스트레스하에서의 생장 패턴, 카드뮴 흡수능을 분석하였다. 카드뮴 100 μM 처리시 E. coli에 발현된 MT유전자들 모두 카드뮴에 내성을 보였고, 각 시간대 별로 MT유전자들의 생장 패턴을 비교해 본 결과, 생장이 미비한 대조구와 달리 MT유전자들 모두 높은 생장능을 나타냈으며, 그 중에서도 Ara-MT2가 가장 높은 생장능을 나타냈다. 카드뮴에 대한 흡착능은 ICP를 통해 분석하였고, 그 결과 Mod-MT2의 경우 254.25 ㎍/㎎으로 가장 높은 카드뮴 흡착능을 보였다. 이는 대조구 43.7 ㎍/㎎에 비해 무려 5배나 높은 수치였다. 다음으로 Ce-MT2와 Ara-MT2 순으로 흡착능을 보였다. 카드뮴 스트레스하에서 가장 높은 생장능을 보였던 Ara-MT2는 흡착능력에 있어서는 오히려 가장 낮은 효과를 보였다. 이 결과를 통해 MT유전자들 모두 카드뮴에 대한 내성이 존재하지만, 그 중에서도 Mod-MT2가 카드뮴 흡착능력이 가장 뛰어난 유전자라 예측할 수 있었다. 본 실험에 이용되었던 카드뮴의 내성과 흡착능이 뛰어난 MT유전자들 중 Ce-MT2를 형질전환 식물 개발에 이용하기 위하여 35S promoter를 이용한 식물발현 벡터를 제작한 후 담배에 형질전환 하였다. 이렇게 생산된 형질전환 식물은 PCR과 western blot 분석방법을 이용하여 담배로의 유전자 도입 및 단백질의 발현여부를 확인하였다. Metallothionein (MT) is a protein with low molecular weight. Its 30% of amino acid is comprised of cystein and it plays an important role in chelation of heavy metal. In this paper, we have cloned genes of Ce-MT2 from Colocassia esculenta, and produced genetically modified Mod-MT2. We also have cloned Ara-MT2 from Arabidopsis thaliana. According to the homology test Ce-MT2 has 70% similarity with other plant MTs. Later when each MT genes are over-expressed to E. coli, we seek out the tolerance against cadmium as well as growth pattern and uptake under cadmium treatment. MT genes over-expressed to E. coli with cadmium 100 μM treatment have all shown tolerance to cadmium. After comparing the growth pattern of MT genes with an hour interval, all MT genes have shown high level of growth capability while control genes have not. ICP was used for analyzing the uptake of cadmium, and as a result, Mod-MT2 has the highest uptake of cadmium with the record of 254.25 ㎍/㎎. This result is 5 times higher than the control with 43.7 ㎍/㎎ in terms of uptake, followed by Ce-MT2 and Ara-MT2. Ara-MT2 with the highest growth capability under the cadmium treatment, and has the lowest effect regarding uptake. As a result, we have found that all of MT genes has the cadmium tolerance, and among those, Mod-MT2 is the gene with best uptake. In order to utilize Ce-MT2 among MT genes with good tolerance and uptake for developing transgenic plant, we have transformed plant expression vector to tobacco under the control of an 35S promoter. The gene insertion of the Ce-MT2 in tobacco was assayed by PCR analyses using genomic DNA. Also protein levels in the transgenic lines were detected by western blot analysis.

酵素法에 의한 輸入新聞古紙의 脫墨時 過酸化水素 漂白이 脫墨펄프의 白色度와 强度的 性質에 미치는 影響

최용복 서울大學校 1991 국내석사

소나무의 정상잎, 피해잎 및 솔방울의 테르페노이드 성분분석

최추이부 江原大學校 1993 국내석사

Gloeophyllum trabeum 으로부터 단리 정제된 Bi-functional Acidic Endoglucanase (Cel5B)의 특성 분석

김호명 전남대학교 대학원 2012 국내석사

The endoglucanase (Cel5B) from the filamentous fungus Gloeophyllum trabeum was cloned and expressed without a signal peptide, and alanine residue 22 converted to glutamine in Pichia pastoris GS115. The DNA sequence of Cel5B had an open reading frame of 1,077 bp, encoding a protein of 359 amino acid residues with a molecular weight of 47 kDa. Based on sequence similarity, Cel5B displayed active-site residues at Glu175 and Glu287. Both residues lost full hydrolytic activity when replaced with alanine through mutation. Following a comparative, specific activity analysis with other endoglucanases and cellobiohydrolase, the enzymatic properties of the purified recombinant Cel5B showed very high specific activity at pH 3.5 and 55°C, about 80-1000 times and 13-70 times on carboxymethylcellulose and filter paper, respectively. Cel5B displayed bi-functional characteristics under acidic conditions. The kinetic properties of the enzyme using a Lineweaver-Burk Plot indicated that Cel5B is a catalytically efficient cellulolytic enzyme.