http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Prakash Annamneedi V (검색결과 1 건)
-
The role of Per2, one of circadian genes, in Atopic dermatitis
Prakash Annamneedi V 삼육대학교 2021 국내박사
Every organism has circadian rhythms which are generated from SCN and helps to the organism to know time of day in order to anticipate and respond to changes in the outer environment. Inflammation is a primary response of body for infection, injury or foreign bodies offense and it is critical for both innate and adaptive immunity. Inflammation disrupted the circadian rhythms results these are promoting inflammatory diseases (Cavadini et al., 2007; Kwak et al., 2008; Lundkvist et al., 2002). Per2 is core circadian gene to generate circadian rhythms and it is associated with enhancing inflammation and inflammatory diseases. Circadian clock disruption in immune cells may promote higher levels of inflammatory cytokines. These abnormal circadian rhythms effect on sleep cycle and it is one of the cause of Atopic dermatitis. In this study we investigated the Per2 role in Atopic dermatitis by establishing Per2-/- DNCB (2,4-Dinitrochlorobenzene) mice and siper2 transfected RAW 264.7 cells. We focused on the Per2 gene involvement in Atopic dermatitis (AD) pathogenesis and macrophage inflammation. And Per2 effect on anti-inflammatory clock gene Brain and muscle ARNT-like 1 (BMAL-1) and Th2 transcription factor GATA-binding protein 3 (GATA3). The Clock gene Per2 has an important role in inflammation and it is enhancing inflammatory cytokines. Atopic dermatitis (AD) is a chronic inflammatory skin disorder associated with defective skin barrier. AD is also a Th2 mediated allergic skin disease. In this study we investigated the relation of Per2 with BMAL1(Brain and muscle Arnt-like protein-1) and the role of Per2 in Atopic dermatitis by animal model and inflammation in macrophages in vitro. We established the atopic dermatitis animal model in Per2-/- and Per2+/+ (wild-type) by treatment with 2,4-dinitrochlorobenzene (DNCB). A subset of mice in both Per2-/- and Per2+/+ were treated with saline as control group. It seems that Per2 in wild type mice enhances the Th2 cell differentiation by upregulation of GATA3 (GATA Binding Protein 3) by suppressing BMAL1. Per2-/- mice doesn’t get atopic dermatitis and it is similar like wild type and Per2-/- control groups. The skin severity score was shown mild and Th2 cytokines also too low in AD-induced Per2-/- group. We found that there is a major difference in enhancement of Th2 cytokine levels during AD development between Per2+/+ and Per2-/- mice. Serum IgE was less produced in Per2-/- mice. According these results, we hypothesized Per2 influence to promote atopic dermatitis by inflammation in Per2+/+ mice and Per2-/- mice protected from allergic atopic dermatitis by increasing anti-inflammatory clock gene BMAL1 through supressing GATA3. For in vitro study of Per2 role in RAW 264.7 cell line, we transfected the RAW264.7 cell line with SiRNA by gene silencing. Transfected RAW 264.7 cells doesn’t get inflammatory response while stimulated by LPS. And nitric oxide and inflammatory cytokine production also very low in SiPer2 RAW 264.7 cells than RAW 264.7 cells. We hypothesized that inflammation disrupt and increase Per2 gene levels which results to develops the Atopic dermatitis by suppressing BMAL-1 and inflammation in macrophages. Per2 is playing key role to triggering atopic dermatitis and inflammation in macrophages. Key words: Per2, atopic dermatitis, inflammation.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
