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      • Effects of water extracts of Remannia glutinosa on neuroprotection by inhibitory action of NMDA receptors

        CHOU HUNGCHUN 경희대학교 대학원 2018 국내석사

        RANK : 247359

        The objective of this study was to observe the effects of water extracts of Rehmannia glutinosa on the glutamate-induced inward current that heterologously express human NMDA receptor. The N-methyl-D-aspartate (NMDA) receptor is one of three types of ionotropic glutamate receptors, the others being the AMPA and kainate receptors. It is activated when glutamate and glycine (or D-serine) bind to it, and when activated it allows positively charged ions to flow through the cell membrane. In the present study, we investigated the regulatory effects of the water extracts of Rehmannia glutinosa on the glutamate-induced inward current that heterologously express human NMDA receptor. The current was measured using the two-electrode voltage clamp technique. In this study, we found the water extracts of Rehmannia glutinosa inhibited glutamate induced current in a reversible and concentration-dependent manner. The IC50 was about 51.2 μg/ml. The water extracts of Rehmannia glutinosa action on glutamate induced current was attenuated by increasing glutamate concentration, and was membrane holding potential-dependent. These results show a possibility that the water extracts of Rehmannia glutinosa interacts with NMDA receptor, which was heterologously expressed and that the water extracts of Rehmannia glutinosa on glutamate induced current of NMDA receptor could be one of contributions of Rehmannia glutinosa-mediated neuroprotections.

      • Health monitoring with smart phones

        CHOU, NORY 이화여자대학교 대학원 2011 국내석사

        RANK : 247359

        기술진보의 빠른 성장과 산업화는 사람들의 삶을 안락하고 편리하게 해주었지만 이러한 혁신과 더불어 사람들의 건강에 해로운 영향을 끼치는 요소들이 생겨났다. 기술적 발명품들이 나오면서 사람들은 점점더 바빠져서 자신들의 건강이 어떠한 상태인지를 의학적으로 체크할 시간이 없게 되었다. 본 연구는 바쁜 현대인들이 스마트폰을 모니터링 기기로 활용하여 스스로 펄스옥시미터를 체크하고 건강상태를 확인함으로써 건강한 생활을 영유할 수 있도록 하기위해 시작되었다. 또한 먼 곳에 있더라도 옷에 부착되거나 몸안에 심어진 Wireless Body Area Networks (WBANs)를 통해 사람들의 건강상태를 점검할 수 있도록 하였다. 본 연구에서 저자는 스마트폰 어플리케이션과 호환가능한 안드로이드 SDK 기반의 프로그램을 고안하여 사용자들로 하여금 펄스옥시미터를 사용해서 건강상태를 모니터링 할 수 있게 하였다. 본인은 이 연구에서 다양한 프로그램과 소프트웨어를 테스트하고 구현해 봄으로써, 되도록 단순하고 구체적이며, 사용자들이 쉽게 메뉴얼을 이해하고 실행버튼을 횔용할 수 있게 하였다. 더 나아가 저자는 본 연구의 타당성과 적용성을 증명하기 위해 WBANs 네트워크 와 펄스 옥시미터, 그리고 안드로이드 SDK에 관해 연구하였다. 이 연구를 진행하면서 저자는 스마트폰 어플리케이션이 잘 만들어진 유저 인터페이스와 WBAN 실시간 네트워크 안에서, 다양한 바디 센서들로부터 얻어진 스트리밍 데이터에 기반한 복합적인 상태들을 모니터링할 수 있다는 것을 알게 되었다. 펄스 옥시미터 센서 툴은 사용자의 건강 상태를 모바일 폰에 입력하기위해 사용되었다. 사용자의 입력은 축적되고, 분석되고 저장된다. 그 후에 사용자들은 건강상태의 처리 결과로써 경고 메시지를 받게 된다. 유저 인터페이스를 활용한 스마트폰 안드로이드 어플리케이션이 WBAN 환경에서 스트리밍 바디 센서 데이터를 위한 모니터링 시스템과 함께 사용될 때, 사용자들의 건강상태를 모니터링하는 것 뿐 아니라 헬스케어, 비상 모니터링, 육체 훈련을 포함하는 광범위한 영역의 어플리케이션들에 활용될 수 있을 것이다. The rapid growth in technological advancement and industrialization of the world made the people’s lives comfortable and convenient yet alongside of these innovations are the detrimental effects to the health of people. As technological inventions advanced, more and more people become busy to the extent that they no longer have time to have their medical check-ups to check whether their health is still conditioned or not. This research was created for the purpose of helping busy people to live a healthy life with the use of Smart Phone as a monitoring device to measure the Pulse Oximeter of the users’ health condition. Hence, even if they are in remote places, their health condition can still be monitored through the use of Wireless Body Area Networks (WBANs) attached to their clothes or implanted in their body. In this research, the author designed a programme using Android SDK that is compatible with smart phone applications in monitoring the health condition of the users with the use of Pulse Oximeter. This study was carried out through testing and implementation different programmes and software to come up with a simple, specific and easy to understand manual and command button for the users. Furthermore, the author had researched the net about WBANs, Pulse Oximeter and Android SDK to qualify the feasibility and applicability of the research. In carrying out the research, the author discovered that the smart phone application could perform the monitoring of complex conditions on streaming data from various body sensors within a WBAN in real time with a nice user interface. The Pulse Oximeter sensor tool was used to input the users’ health condition in the phone. The inputs are stored, analyzed and saved. After that, the users will receive an Alert Message as a result of their processed health condition. The author found out that a smart phone android application with an advance user interface can be used in monitoring health condition together with a monitoring system for streaming body sensor data in a WBAN environment and also support a wide range of applications including healthcare, emergency monitoring and physical training.

      • Engineering properties of lignosulfonate-polyethyleneimine treated granular soil

        Chou, Sopharith Graduate School, Korea Unversity 2020 국내석사

        RANK : 247359

        Recently, lignosulfonate (LS) has been reported to have potential as a soil stabilizer in the geotechnical engineering field since it is not only an environmentally friendly and non-toxic material but could improve the mechanical properties of soil as well. However, lignosulfonate has high hydrophilic characteristics which causes removal from treated soil into the water. According to this deficiency, the additional admixture is required in an effort to overcome this limitation. In this study, polyethyleneimine (PEI) is mixed into lignosulfonate solution to stabilize crushed silica sand in order to investigate the improvement of the water-resistant and mechanical strength characteristic of lignosulfonate treated soil through the performance of laboratory tests. Unconfined compressive strength (UCS), wetting-drying cyclic, consolidated undrained triaxial, hydraulic conductivity, wind erosion, and soil pH tests are conducted. The UCS test of treated soil is assessed according to three main factors: lignosulfonate concentration, content, and soil relative density. As a result, it is shown that the UCS shows an increasing trend with a rise of lignosulfonate content. Moreover, the UCS is influenced by the drying out of the water from a specimen which is relevant to the study of UCS with varying the lignosulfonate concentration and curing time. It is seen that the higher the concentration and the longer the curing period result in an increase of UCS. In addition, the optimum soil relative density is revealed according to the variation of density has an effect on the void volume and ratio of binder to sand particles. For the wetting-drying cyclic test, the disintegration of the specimen is not observed for the case of the sample treated with 1 to 1 (PEI:LS) binder. Although the compressive strength of treated soil at wet condition shows a notable decrease, after re-drying for 7 days in a controlled room, its strength recovers about 90% of its initial dry condition. From the triaxial test, the deviatoric stress increases with the increase of lignosulfonate content. For treated soil, both cohesion and elastic modulus are improved, and it behaves more ductile compared to soil treated by other stabilizers. Additionally, LS-PEI binder also influences the permeability of treated soil by reducing about 2 orders of hydraulic conductivity. Besides, from the wind erosion test, it could be seen that LS-PEI binder improves the wear resistance and moisture retention of soil as well since the mass and moisture loss decreases with the increase of LS-PEI binder. Lastly, soil treated by LS-PEI shows relatively lower pH compared to the soil treated by other traditional soil stabilizers; so that it does not threaten the environment such as underground water and soil since it does not make the corrosive atmosphere. To conclude, polyethyleneimine improves the water-resistant and mechanical properties of lignosulfonate treated soil, and LS-PEI could be a new alternative soil stabilizer for soft ground improvement and a sustainable solution for geotechnical engineering application in the future.

      • Evaluation of intrathecal administration of human regulatory T cell in Alzheimer’s disease mouse model

        CHOU HUNG CHUN 경희대학교 대학원 2021 국내박사

        RANK : 247359

        Neuroinflammation is primary feature of Alzheimer’s disease (AD) pathology. For few decades as now, AD research continues to be driven by developing novel and effective AD immunotherapy. Currently, the interest in AD immunotherapy is accelerated by a specific subset of T cells, CD4+CD2+Foxp3+ regulatory T cells (Treg), suppressing autoimmunity and maintaining immune homeostasis. Here, we demonstrated the therapeutic impact of human Treg cells on AD in a murine model of Alzheimer’s disease. Freshly isolated and ex vivo expanded human Treg (hTreg) cells were intrathecally (i.t.) administered in 3xTg Alzheimer’s disease mouse model. Importantly, such therapeutic potential of hTreg cells was significantly suppressed increased microglia activity. These results suggest that hTreg cells may be involved in modulation of neuroinflammation in AD pathogenesis by slowing or suppressing microglial response associated with Ab toxicity. Our study highlights the potential contribution of adoptive transfer of hTregs as live drugs for innovative immunotheray against Alzheimer’s disease and an approach to modulating neuroinflammation.

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