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RISS 인기검색어
- 검색키워드
- 저자 : 폰카즈 쿠마르 바닉 (검색결과 1 건)
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폰카즈 쿠마르 바닉 전남대학교 대학원 2012 국내석사
Objective: To investigate the replacement and trophic actions of neural stem cells after transplantation into the hearing impaired guinea pig by acoustic trauma. Materials and Methods: Acoustic trauma was induced by a continuous white noise at 120 dB SPL for 4 h/day for 3 consecutive days. Three days after noise injury human neural stem cells (HB1.F3) were transplanted into the scala tympani of the basal turn. Phalloidin staining and scanning electron microscopy were carried out to detect the status of hair cells of the organ of Corti. Immunohistochemistry with specific antibodies, toluidine blue and hematoxylin and eosin staining were used to demonstrate the spiral ganglia neurons. Hearing ability was assessed by auditory brain stem response (ABR) evaluation. Neutrophic factors (Glial cell line-derived neurotrophic factor as GDNF, angiopoietin-3, and Neurotrophin-3) were analyzed by western blot analyses. Results: The loss of hair cells within the organ of Corti and degeneration of spiral ganglion neurons after noise injury was found at-turn. Thresholds of click evoked ABR and 8 kHz tone burst ABR were increased to 60-75 and 60-80 dB, respectively. After stem cell engraft, the number of cochlear hair cells was increased in the 2nd and 3rd turns of cochlea compared with control group under phalloiding staining and SEM. The number of neuronal cells in the spiral ganglia was also increased in transplanted group. In addition, fluorescent immunohistochemistry with human specific antibody (hNuclei) revealed that implanted human neural stem cells migrated and survived with in the spiral ganglia. Moreover, in toluidine blue, hematoxylin and eosin staining, findings were demonstrated the increased number of neuronal cells in spiral ganglia in transplanted group than control group. Furthermore, after transplantation of neural stem cells significant increase in the expression of neurotrophic proteins such as GDNF, NT3 and angiopoietin 3 was observed in cochlea. ABR results showed the hearing recovery after transplantation. Thresholds of click evoked ABR and 8 kHz tone bursts ABR were decreased to 25-40dB and 35-45dB, respectively. Conclusion: Present study has demonstrated that neural stem cells are capable to replace the damaged spiral ganglion cells and to preserve hair cells in noise-induced hearing loss model, and stem cell transplantation also promotes hearing recovery
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