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RISS 인기검색어
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- 저자 : 백진원 (검색결과 3 건)
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배양한 흰쥐 대뇌세포의 저산소증모델에서 蘇合香元이 유전자 표현에 미치는 영향
The purpose of this investigation is to evaluate the effects of Sohaphyang-won(SH) on the alteration in gene expression in a hypoxia model using cultured rat cortical cells. E18 rat cortical cells were grown in Neurobasal medium containing B27 supplement. On 12 DIV, SH was added (20㎍/㎖) to the culture media for 24 hrs. On 14 DIV, cells were given a hypoxic insult (2% O_(2)/5% CO_(2), 37°C, 3 hrs), returned to normoxia and culture for another 24 hrs. Total RNA was prepared from SH-untreated (control) and -treated cultures and alteration in gene expression was analyses by microarray using rat 5K-TwinChips. Effects on some of the genes whose functions are implicated in neural viability are as follows: 1) For most of the genes altered in expression, the Global M values were between -0.5 to +0.5. Among these, 1517 genes were increased in their expression by more than Global M +0.1, while 1480 genes were decreased by more than Global M -0.1. 2) The expression of apoptosis-related genes such as Bad (Global M = 0.35), tumor protein p53 (T53) (Global M = 0.28) were increased, while v-akt murine thymoma viral oncogene homolog 1(Akt1) was decreased. 3) The expression of hemoglobin alpha 1 (probably neuroglobin) was increased by about 3.2-fold (Global M = 1.7). 4) The expression of antioxidation-related catalase gene was increased (Global M = 0.26). 5) The expression of PKCzeta(prkcz), an upstream kinase of MAPK, was increased (Global M = 0.29). 6) The expression of retinoic acid receptor alpha (RARα), which may regulated transcription in hypoxic stress, was increased (Global M = 10.27). In summary, the microarray data suggest that SH doesn't increase the expression of oxygen capture-, anti-oxidation- and 'Response to stress'-related genes but also decreases some anti-apoptosis genes which would help protect the hypoxiv cells from apoptosis.
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丹蔘散이 apolipoprotein E 결손 당뇨병유발 생쥐의 당뇨병, 동맥경화증 및 고콜레스테롤혈증에 미치는 영향
Background Diabetis mellitus(DM) showed sudden increase recently is tend to accompany with Atherosclerosis and Hyperlipidemia. Melitus-Atherosclerosis-Hypercholesterolemia caused by interaction of DM, insulin resistance, oxidative stress and lipid metabolic abnormality develop a complication. Objective We examined with Apolipoprotein E -/- Diabetic Mouse to investigate the effects of Dansamsan(DSS) on Diabetic Melitus-Atherosclerosis -Hypercholesterolemia. Methods ApoE -/- mouse were classfied into three groups: control group, ApoE -/- diabetic mouse(AD group, high fat diet and STZ) and ApoE -/- diabetic mouse with DSS treated group(DT group). Present study was carried out to investigate the curative effects of herb-combined prescription Dansamsan(DSS) on Diabetic Melitus-Atherosclerosis-Hypercholesterolemia in ApoE -/- diabetic mouse. The effect of DSS extract was observed by measuring 3 parts. 1. diabetic mellitus : the changes of blood glucose and recovery of β-cell on pancreatic islet. 2. atherosclerosis : the change of deposit of fat at aortic arch, CD36, PPAR-г, and PDGF-C 3. Hypercholesterolemia: the change of body weight, total cholesterol, LDL and lipid distribution of liver and kidney. Results 1. DSS extract showed effective affects on decline of blood glucose and It recover the insulin secretion on Pancreatic islet β cell. 2. DSS extract showed effective affects on decrement of deposit of fat at aortic arch, suppression the formation of foam cells-inhibiting the CD36, PPAR-γ and effect against formation of proliferation of smooth muscle cell-decrease of PDGF-c reaction 3. DSS extract showed effective affects on increase of body weight, decrease of total cholesterol and LDL cholesterol. Finally we found the decline of lipid distribution of liver and kidney tissue. Conclusion The results may suggest that DSS extract can improve Diabetic Melitus-Atherosclerosis-Hypercholesterolemia at the same time, by control of blood glucose, lipid metabolism and antioxidant effect.
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