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개 후각망울에서 칼슘결합단백질인 Parvalbumin과 Calbindin D-28K 면역양성반응 신경세포에 관한 연구
We have studied the distribution of calcium-binding proteins parvalbumin(PV) and calbindin D-28kCB) immunoacitivity in the mongrel dog olfactory bulb using monoclonal antibodies and the avidin-biotin-immunoperoxidase method. The possible coexistence of both markers was determined by segmental histochemical and immuohistochemical double labelling of the same section. In the olfactory bulb of mongrel dog, PV-IR and CB-IR were mainly located in the external plexiform layer, and a few scattered in the glomerular layer, mitral cell layer, and glanule cell layer in the order of their existence. In addition, three neuronal types were observed in the glomerular layer and external plexiform layer border. Horizontal cells and vertical cells of Cajal were also observed after both PV-IR and CB-IR labeling. Distict groups of PV-IR and CB-IR, differing in size, shape, dendritic branching pattern, and staining intensity, were distinguished in the all layers of the olfactory bulb. Specific neuronal populations were positive for both PV-IR and CB-IR markes. No cell colocarized both stains in the mongrel dog olfactory bulb. The number of PV-IR were more than abundent in olfactory bulb compared to the CB-IR cells. The PV-IR and CB-IR positive, cell somata were round, oval, spindle and polygonal in shape, and the positive neurons were unipolar, bipolar, multipolar and horizontal in shape. The diameters of the somata of the two positive neurons were 20-40㎛, respectively. Also dendrites of two positive neurons were densely arrayed in arborization.
포유동물의 전맥락총 동맥벽의 탄성섬유 분포에 관한 비교형태학적 연구
A comparative histological study was carried out to observe the distribution of the elastic fibers contained in the intima, internal elastic membrane, media and adventitia in the anterior choroidal artery walls of 8 species of mammalia including mouse, rat, gold hamster, guinea pig, rabbit, squirrel, roe deer and cow. The obtained results were as follows : 1. The internal elastic membranes in the anterior choroidal arteries were relatively well developed in all the species. 2. The weve numbers of the internal elastic membrane were most numerous and thickest in the cow and roe deer. 3. The highest development of the wave was observed in the anterior choroidal artery of the guinea pig. 4. No external elastic membranes were found being scattered fine irregular elastic fibers in the media and the adventitia.