지하철 진동이 주변지반 및 인접구조물에 미치는 영향

강호정 漢陽大學校 1986 국내석사

알레르기성 비염환자의 부비동조직에서 IL1, 4, 5의 발현에 대한 면역조직화학적 연구

강호정 東亞大學校 大學院 2000 국내석사

배경 : 알레르기성 비염과 만성 부비동염이 동반된 경우를 임상에서 흔히 경험하게 되는데, IgE와 관련된 제 1형 과민성반응이 만성부비동염의 병인에 영향을 미치는 것으로 알려져 있으나 이의 실험적 증명은 충분히 이루어지지 않았다. 목적 : 알레르기성 비염이 동반된 만성 부비동염 환자의 부비동 점막내에서 IL1, 4 및 5의 발현유무 및 정도를 면역조직화학법을 이용하여, 비알레르기성 환자군과 비교함으로써 만성 부비동염의 병인에 미치는 알레르기성 반응의 역할을 규명하고자 하였다. 재료 및 방법 : 본원 이비인후과를 내원한 환자중 증상, 국소소견 및 방사선학적 소견상 만성 부비동염으로 진단되어 부비동 내시경수술을 시행받은 환자 10명을 대상으로 하였다. 이들 중 5명은 알레르기성 비염의 병력이 있고 MAST검사 및 알레르기 피부반응 검사에서 알레르기성 비염으로 진단받은 환자였고(실험군), 나머지 5명은 알레르기검사상 알레르기비염의 소견이 없는 만성 부비동염 환자였다(대조군). 두 군 모두에서 H&E 염색 및 cytokines(IL1, 4, 5)에 대한 면역조직화학검사를 시행하여, 침윤하고 있는 호산구세포의 수치 및 cytokines의 발현유무 및 정도를 관찰하였다. 결과 : 알레르기환자군에서 호산구수치는 120±60으로 비알레르기군의 5±1보다 높게 관찰되었다. IL1은 주로 혈관조직과 상피조직에서 양성으로 염색되고, 점막하 세포에서도 일부 염색되었는데, 두군에서 유의한 차이는 없었다. IL5는 주로 점막하세포와 상피조직에서 양성으로 염색되고, 알레르기군이 비알레르기군보다 높게 발현됨이 관찰되었다. IL4는 두 군 모두에서 염색이 되지 않았다. 결론 : 비록 대상자수가 적어 통계학적 의의는 적어나, 부비동 점막을 대상으로 한 본 연구에서도 알레르기성 부비동염의 병인에 있어서 제 1형 과민성 반응의 역할이 중요함을 확인할 수 있었다. We often encounter allergic rhinitis patients with an accompaning sinusitis. Typer Ⅰ allergies are believed to be involved in allergic sinusitis. However there is not enough evidence for this. We purposed to determine the role of IgE mediated allergic reactions in allergic sinusitis by investigating the immunohistochemical differences of IL1,4,5 between patients with allergic sinusitis and control subjects with non allergic chronic sinusitis. Ten chronic paranasal sinusitis patients confirmed by symptom, local finding and radiologic finding were included for this study. All patients underwent endoscopic sinus surgery and ethmoid sinus mucosa was taken for specimen. Among them, five allergic patients were selected by history of allergic rhinitis and confirmed by positive allergic reactions in MAST and skin tests. Specimens were stained and we measured the number of eosinophils and the concentration of cytokines(IL 1,4,5). The number of eosinophils was higher in paranasal sinus mucosa front patients with allergic sinusitis IL1 positive cells were expressed in epithelium and vessels, and partially submucosal cells. But There was no significant difference in concentration of Ⅲ between two groups. IL5 positive cells were expressed in vessels, submucosal cells, and the concentraion of IL5 was higher in the mucosa of allergic sinusitis. Expression of IL4 was not detected in both groups. 12. Yamaguchi Y, .Suda T, Sudal et al : Purified interleukin-5 supports the terminal differentiation and proliferation of murine eosinophilic precursors. J Exp Med 167:43-56 (1988) 13. Shirasaki H, Asakura K. Narlta s et al : A study of experimental allergic sinusitis on guinea pigs. Oto Rhinol Laryngol(Tokyo) 38:232-237 (1995).

Avian myeloblast virus reverse transe transcriptase 활성 저해 세균의 분리

강호정 濟州大學校 大學院 2008 국내석사

To screen antiretroviral compounds from microbial metabolites, 300 soil microorganism strains focusing Actinomycete family were collected and purified in Jeju. Those microbial isolates were cultivated and their culture filtrates were used as the samples for screening antiretroviral compounds. Inhibitory activity of microbial culture filtrate against avian myeloblast virus(AMV) reverse transcriptase (RT) was determined. About 87% of the 300 isolates showed AMV RT inhibitory activity in the primary screening test. Those isolates that showed higher than 60% inhibition of RT activity were chosen and their inhibitory activities were confirmed repeatedly using their cell-free culture filtrate. The inhibition rates of BLK268, BLK115, BLK418 and BLK338 were 98.7± 0.3%, 97.9± 0.2%, 97.9± 2.7%, and 95.9± 1.6%, respectively. The isolates BLK311, BLK90, BLK462-2, BLK248-2, CB249-2, and BLK107 showed morer than 80% inhibition, and BLK4, BLK86, BLK123, and BLK39 higher than 70% inhibition of AMV RT activity. Among those isolates, six strains including BLK39, BLK90, BLK107, BLK115, BLK268 and BLK418 were chosen for further study. In order to confirm the dependence of the inhibitory activity on microbial metabolite concentration, the cell-free culture filtrates were diluted several folds and added to the RT activity assay system. The results demonstrated that RT inhibition rate increased in general as the dilution rate decreased. The heat stability of active microbial metabolites were also investigated. When the culture filtrates were preincubated for 1 hour at 30℃ or less, no decrease in the inhibitory activity was observed. But most of the isolates lost the inhibitory activity rapidly at temperatures higher than 50℃, whereas the isolate BLK90 maintained relatively high inhibitory activity at above 50℃. The unknown isolates were identified through 16S rRNA gene analysis. The nucleotide sequences of 16S rDNA from BLK39, BLK90, BLK107, BLK115, BLK268 and BLK418 were determined and compared with those of other known microbial species. The isolates BLK 39, BLK115, BLK268 and BLK418 showed more than 96% sequence homology with Streptomyces species. Meanwhile, the 16S rDNAs of isolate BLK90 and BLK107 were similar to those of gram negative bacteria which belong to the genus Erwinia or Pantoea. Morphological features of these bacteria were consistent with the results of rDNA sequence analysis. Further research about inhibition mechanism and chemical nature of those bacterial metabolites are suggested.

새의 조형성에 관한 연구 -장신구 제작을 중심으로-

강호정 창원대학교 2015 국내석사

인간의 삶은 탄생의 순간부터 많은 관계 속에서 살아간다. 가족, 친구, 동료, 사회적인 모임이나 단체에 소속되어 본인이 원하던 원하지 않던 사회의 일원으로 긴 삶을 살아간다. 이 사회적인 인간관계 속에서 우리는 즐거움, 슬픔, 분노, 기쁨과 같은 여러가지 감정의 소용돌이 속에서 인생이란 여정을 걷고 있다. 또한 자신들이 속한 사회, 가정, 단체 등에서 많은 책임과 의무에 이끌려 자아에 의해 때론 타인에 의해 주어진 환경의 틀에 갇혀서 길들여지고 그것을 당연한 숙명처럼 받아들이면서 삶을 살아가고 있다. 보람되고 행복도 느끼겠지만 누구나 한번 씩 책임과 의무라는 족쇄에서 벗어나 맘껏 자유를 꿈꾼다. 현대 사회를 살아가는 한 일원으로써 이와 같은 삶의 지루함이나 속박에서의 일탈을 꿈꾸고 자유에 대한 욕망을 갈구하고 희망이라는 날개 짓을 염원하기도 한다. 새는 하늘을 날 수 있는 동물로써 예로부터 인간에게 동경의 대상이 되어왔다. 그리고 생김새가 가녀리고 연약한 존재로 새의 이미지가 다채로운 모습으로 표현하기에 충분하고 인간의 욕망을 대신해 줄 수 있는 매개체로 복잡한 인간의 감정을 표현하기에 충분한 소재라고 판단된다. 본인이 연구하는 작품은 새의 모티브를 전제로 비상과 희망이라는 상징성을 벗어나서 비상을 꿈꾸는 인간의 생활모습 즉, 언제나 반복되는 일상, 가족에 일에 때론 국가에 대한 책임과 의무에 힘들어 하고 숙명처럼 살아가는 우리들의 일상생활을 표현하고자 한다. 또 그 속에서 작으나마 나의 존재를 표현하고 그곳이 부정적인 면만 있는 것이 아니고 작은 어울림 속에서의 조화도 있으며, 책임과 의무를 다하여 이루어낸 보람과 자부심도 있고, 인간관계에서의 사랑과 정이라는 따스함도 표현하였다. 작품에서의 중심은 인간이다. 인간을 새의 형상을 통하여 표현하여 인간의 삶을 비추어 재조명하는데 목적을 두고 있기 때문이다. 작품제작에 있어서 새의 형태를 사실적으로 묘사하기 보다는 새가 가진 유선형을 강조하여 간결하고 단순화시켜서 조형적 요소와 특징을 강조하여 금속의 차갑고 딱딱한 소재를 부드럽고 따스함을 전달하는데 목적을 두고 때론 어우러지게 때론 숨어 버리게 때론 튀어나오게 포근하게 감싸 안긴 듯 표현하여 새를 통해 삶의 형태를 고찰하고자 하였다. 이와 같은 연구는 앞으로의 작업 방향과 삶에 있어서 자아성찰과 자아실현을 위해 끊임없이 노력하고 고뇌하는 인간의 본성에 대한 연구를 바탕으로 자아를 완성하고 성숙되어 삶에 대한 올바른 인식과 태도를 본인을 포함한 많은 이들에게 인식되는 기회가 되기를 바란다.

폐폴리우레탄폼의 자연발화특성에 관한 연구

강호정 부경대학교 산업대학원 2002 국내석사

지적장애인의 부모가 인식하는 자녀의 승마 참여제약 요인

강호정 한국체육대학교 대학원 2013 국내석사

섬기린초에서 분리한 항산화 및 항염증 물질 : Antioxidant and Antiinflammatory compounds from Sedum takesimense Nakai

강호정 충남대학교 2006 국내박사

Sedum takesimense Nakai is an endemic species in Korea, and there is no report on its constituents and biological activities. One of related plants, Sedum kamtshaticum has been used as a folk medicine for anti-inflammation, and improving the circulation of blood. In this study, the methanol extract and solvent fractions of S. takesimense were ---------------------------------------------------------------- * A thesis submitted to the committee of Graduate School, Chungnam National University in a partial fulfillment of the requirements for the degree of doctor of Philosophy conferred in August 2006. measured on several radical scavenging activities and inhibitory effect of COX-1 and COX-2. Among them, ethyl acetate and butanol fractions which showed strong anti-oxidant activity and relatively effective inhibitory COX-1 and COX-2 effects were selected to isolate the bio-active constituents. In order to isolate bio-active constituents, fresh whole plant of S. takesimense (2.5 kg) was refluxed with MeOH for 4 h. The MeOH extract (460g) was suspended in water and then partitioned with hexane, ethyl acetate, and butanol, sequentially. The IC50 (μg/ml) values of DPPH and superoxide radical scavenging activity of ethyl acetate fraction were 4.6 and 0.7, and the IC50 (μg/ml) of butanol fraction were 5.2 and 0.7, respectively. Additionally, COX-1 and COX-2 inhibitory effects of ethyl acetate fraction were 84.7% and 91.6% at 200 μg/ml as the final concentration. Furthermore, COX-1 and COX-2 inhibitory effects of butanol extract were 68.8% and 75.7%. Therefore, these two fractions selected to isolate the bio-active constituents. Sephadex gel and silca gel column chromatography, and preparative HPLC of the active fractions led to the isolation of fourteen compounds including three new compounds. They were identified as ferulic acid (1), caffeic acid (2), gallic acid (3), methyl gallate (4), 1-(4-hydroxyphenyl) - 2-(3,5-dihydroxyphenyl)2-hydroxyethanone (5), myricetin (6), quercetin (7), luteolin (8), rhodalin (9), gossypetin-8-O-β-D-xyloside (10), rhodalidin (11), luteolin-7-O-β-glucoside (12), 2,6-di-O-galloylarbutin (13), and arbutin (14) by comparing spectral data with those previously reported. Among them, compound 5, 10, and 13 were isolated as new compounds. The isolated compounds were tested for their anti-oxidative activities with DPPH and superoxide radical scavenging assay system. 2,6-di-O-galloylarbutin (13) showed strongest DPPH radical scavenging activity and the IC50 value was 3.63 μM. Gossypetin-8-O-β-D-xyloside (10) also showed prominent superoxide radical scavenging activity and the IC50 value was 5.5 μM. These two new compounds have effective scavenging activity on each other free radical. In the NO radical scavenging assay, well known compounds such as caffeic acid and luteolin showed relatively effective NO radical scavenging activity. On the basis of these antioxidant activity, three new compounds from S. takesimense were evaluated for inhibitory effects of LDL-oxidation. As results, IC50 values of 10 and 13 in TBARS assay were 5.7 and 3.3 μM, respectively. In addition, the lag time (10: 72 and 180 min 13: 80 and 170 min, probucol: 76 and 120 min at 1 and 3 μM, and control: 41 min) and the REM (inhibition of 10 and 13: 100% and 38% at 10 μM). In conclusion, the antioxidant activity of 2,6-di-galloylarbutin (13) showed more potent than positive controls in these assay. Two new compounds, 10 and 13 showed strong inhibitory effects on COX-1 activity with the IC50 values of 32.2 and 55.7 μM, respectively. Meanwhile, compound 5 exhibited supressive activity against both COX-1 and COX-2 activities, with the IC50 values of 39.8 and 61.4 μM. From these, the anti-inflammatory action of three new compounds might be related to the inhibitory effect of cyclooxygenase. In addition, the inhibitory effect of LPS-induced NO and ROS production in RAW 254. 7 cell and cell viability for 5, 9, 10, and 13 were evaluated. All compounds show no cytotoxicity to RAW 264.7 cells. Compound 9, 10 and 13 showed inhibitory effect of LPS-induced ROS production; the percent inhibitions of ROS production were 19, 39 and 71% for 10, 39, 53 and 69 % for 13, 66, 66 and 72 % for 9 at 50, 100, and 200 μM , respectively. Three new compounds showed inhibitory effect of TPA-induced mice ear edema and lymph node in vivo. The percent inhibition of TPA-induced mice ear edema for 5, 10, and 13 were 65.3, 48.7, and 69.7 %, respectively. Additionally, the percent inhibition of TPA-induced mice lymph node's weight for 5, 10, and 13 were 66.7, 33.3, and 33.3 %. However, it was expressed that there was no remarkable inhibition of TNF-α formation except for 13, which showed 21.7 % inhibition on TNF-α formation.

인체 피부표면의 지질양과 지질구성성분의 분석에 관한 연구

강호정 이화여자대학교 대학원 1996 국내석사

인체의 피부표면지질은 phospholipids, sphingolipids, choesterols, cholestrolesters, fatty acids, triglycerides, squalene과 wax ester로 구성되며 이들은 체액손실을 막고 경피흡수를 조절하는 외에도 각질 형성세포간의 결합과 탈락 및 표피분화와 성장조절에도 관여한다고 알려져 있다. 본 연구는 정상인과 여드름 환자를 대상으로 피부표면지질의 추출유기용매와 신체부위에 따른 변화를 알아보고 이러한 지질변화가 건조한 피부와 여드름 발생에 미치는 영향을 알아보기 위하여 전자미세저울로 지질의 양을 측정하고 그 구성성분은 high performance thin layer chromatography와 photodensitometer로 측정한 결과는 다음과 같다. 1.Hexane, hexane/methanol(2:3, 용적비) 및 95% ethanol 세가지 용매에 따른 지질추출량은 통계적으로 유의한 차이가 없었다. 다른 용매에 비해 hexane으로 추출시 cholesterol-3-sulfate 및 총 ceramides는 적었고 (p<0.05) triglycerides는 많았다.(p<0.05) 2.이마의 지질량은 다른 부위에 비해 유의하게 많이 추출되었다.(p<0.01) ceramides은 뺨에서 많이 추출되었고 (p<0.05) cholesterol은 다리에서 많았으며 다리나 뺨보다 복부에서 triglycerides는 많았다. Cholesterol esters와 wax esters는 다리에서 뺨과 이마부위보다 적었다(p<0.05). 3.건조한 피부에서의 지질 추출량은 유의한 차이를 보이지 않았다. 건조한 피부보다 매끄러운 피부에서 tryglycerides는 많았지만(p<0.05) 그외 다른 지질구성성분에는 유의한 차이가 없었다. 4.정상인과 여드름 환자에서 지질추출량 및 그 구성성분에 유의한 차이는 없었다. 이상의 결과로 자극성과 홍반발생이 컸던 hexane/methanol 을 사용했을 때 ceramides와 극성지질(polar lipid)인 cholesterol-3-sulfate가 많이 추출되어 ceramides와 극성지질(polar lipid)이 피부장벽기능에 중요한 역할을 할 것이라는 것을 간접적으로 시사하며 겨울철의 피지선 기능감소가 건조한 피부의 원인이 된다고 생각되었다. 여드름 환자는 1차적인 각질층 지질변화에는 영향을 받지않는 것으로 여겨지며, 피부 표면지질의 추출용매에 따른 결과의 해석과 신체부위별 피부표면지질 구성성분의 차이가 피부기초학 연구에 도움이 되리라 여겨진다. The composition and biosynthesis of lipids in human skin have long been of considerable interest. The human skin surface lipids are derived from two principle sources; the sebaceous glands and the epidermis. The composition of skin surface lipids includes phospholipid, sphingolipid, choesterol, cholestrolesters, fatty acids, triglycerides, squalene and wax esters. And it is known that skin surface lipids may play function roles as follows:control of epidermal growth and differentiation, determinination of cohesion and desquamation of corneocyte, formation of lamellar granules and functioning as permeability barrier. Therefore we sat out to find out the differences of skin surface lipids according to the solvents, different anatomic sites and the posible effect of these lipid alteration on xerosis and development of acne. The skin surface lipids was extracted by cup method and analyzed the lipid composition by high perforance thin layer chromatography. The result were as follows: 1.Total amount of lipids extracted with 3 solvents(hexane, hexane/methanol, ethanol) were not significantly different. Hexane/methanol was more powerful extract solvent of epidermal lipids(ceramides & cholesterol ?3-sulfate) than hexane. Hexane/methanol and ethanol was more irritant than hexane. 2.The amount of skin surface lipids on the forehead was higher than that of the other site. The composition of lipids was different according to the sites(total ceramides in cheek and truglycerides in andomen were higher than other sites. Sterol easter and wax esters in leg were lower than other sites) 3.Triglycerides in smooth legs indicates higher proportion than that in xerotic legs. 4.There were no significant differences in amount and composition of extracted lipids between acne patients and normal surbject. In conclusion, these results might ce helpfuul to elucidate the differences of extracted lipid composition depending on the solvents and anatomin sites. Total ceramides and polar lipids may play an important role in the maintenance of the normal barrier function. it is thought that decreased sebaceous function (triglycerides, cholestrol esters and wax esters) would be significantly associated with xerosis of legs. These results suggest that total ceramides primarily have not been implicated in the follicular retention keratinization of acne.

組織構成員의 스트레스에 관한 硏究

강호정 東國大學校 敎育大學院 2002 국내석사

The purpose of this study is to analyze the factors and conditions of the intra-organizational stress to which the members of organization facing a fierce international competition are subject, and the diseases caused by such a stress and thereby, find the ways to counter the stress to arrange some guidelines for effective management of the human resources for organizations. The results of this study can be summarized as follows; First, male and female members do not be frustrated by any stressful situation to discharge his or her duties to the end. However, new comers may be more or less frustrated by a stressful situation. Second, when a member faces a stressful situation on his or her duty, he or she may be considerably angry. Recently, employees tend to discharge their duties in their own ways unlike in the past. So, if they are subject to a stress, it is possible for them to be affected negatively by it. In this regard, supervisors or managers are requested to be skillful enough not to subject their subordinates to a stress when commanding or ordering them to do their duties. Third, regardless of ranks or age groups, a considerable portion of the employees feel stressful for their ordinary duties. Such a finding suggests that most of the employees are always vulnerable to a stress on their duties. Therefore, the organization is obliged to arrange some program to solve its employees' stress, while the employees themselves are requested not to be vulnerable to a stressful situation. Fourth, when an employee is exposed to a stressful situation, he or she may explore the ways to counter it, but may consider leaving the organization seriously, regardless of sexes. Such a tendency may reflect the situation of the time since many companies lay off and recruit the people with some career in the wake of IMF bailout program. Fifth, employees are subject to a stressful situation every day. In other words, they tend to suffer from stress and resolve it every day. Sixth, when employees are subject to a serious stress, they cannot strike a fresh idea or discharge their duties in a creative way. In this age of change and creation or in the age when those with fresh ideas and unique thoughts can survive, paralysis of creative thoughts means 'falling behind' in the competition. Seventh, when employees are subject to a stress, they tend to react sensitively to their colleagues' requests, which means that inter-department cooperation may not be smooth. If such a cooperation is not smooth when a synergy effect should be enhanced to a maximum degree, the performance must be poor. Eighth, many employees suffer from an unbalanced discharge of duties and a sense of instability when they are subject to a stress. When they are unstable, they cannot discharge their duties smoothly, much less achieving a good performance. Ninth, when employees are subject to a stress, most of them feel a pain in their backs, necks or heads. In other words, a considerable number of employees suffer from a chronic stress which may lead to a disease. Since mental stress may result in a physical disease, it is essential to arrange some anti-stress measures and additionally, explore the ways to relieve them of their stress physically. Tenth, many employees suffer from such gastro-intestinal problems as stomach ache or digestive difficulty, regardless of age groups or ranks. So, it is deemed necessary to find the ways to solve such problems for the employees selectively. Eleventh, when subject to a stressful situation, the majority of the employees tend to suffer from fatigue or shoulder pain. Twelfth, some employees often suffer from insomnia or disrupted thinking due to stress. Therefore, it is deemed necessary to arrange some individual measures for such problems. Thirteenth, the majority of male employees tend to rely on fierce exercises, drinking or smoking, while the majority of female employees tend to depend on listening to music or intake of foods. The younger a male employee is, he tends more to stop drinking or smoking, while the number of women rely on sports to be relieved of stress. Such a phenomenon seems to be desirable.

비행성능 최적화를 위한 헬리콥터 로터 설계

姜鎬廷 建國大學校 大學院 2009 국내석사

회전익 항공기는 수직이착륙, 호버링 등 좁은 공간에서의 비행이 가능한 특성으로 인해 산악 지형에 적합한 항공기 형태이며, 민간용이나 군용으로서 활발하게 사용되고 있다. 회전익 항공기는 회전하는 로터에 의해 양력과 추력을 동시 얻는 특성으로 인하여, 고정익 항공기기에 비해 형상 및 성능을 결정하는 요소가 복잡하고 다양하다. 그렇기 때문에 공력, 구조, 로터 동역학 등의 복합적인 분야를 고려하여 해석하는 것이 필요하다. 본 연구에서는 회전익 항공기의 사이징 및 성능해석 프로그램을 개발하고, 트림 프로그램과 연계 해석이 가능하도록 프로그램을 통합하였으며, 통합된 프로그램을 이용하여, 회전익 항공기 로터 블레이드의 최적 형상 설계를 수행하였다. 형상 설계의 변수로는 블레이드의 형상 변수들과 에어포일의 형상을 선정하였으며, 블레이드 형상에 대한 해석은 성능해석 프로그램을 통하여 수행하고, 에어포일 형상에 대한 해석은 panel method 기반의 프로그램을 사용하여 진행하였다. 본 연구를 통하여 개발된 프로세스는 회전익 항공기 전체 형상에 관한 최적화 연구에 적용될 수 있을 것이다. Rotorcraft have a special characteristics about hovering, vertical takeoff and Landing. In this reason, It is suitable aircraft for using the mountainous area. Therefore rotorcraft is used in civil and military. Rotorcraft is obtained Lift and thrust by the rotor. for this reason, configuration and performance selection factors are more complicated then fixed wing aircraft. therefore, multidisciplinary design was required for rotorcraft design that considering the aerodynamics, structure and rotor dynamics. In this study, rotorcraft sizing and performance analysis program is developed. That program is integrated with trim analysis program. Using that integrated program, rotorcraft rotor blade optimum design is performed. blade configuration and airfoil shape are selected on design variables. Blade configuration is analyzed by performance analysis program, airfoil shape is calculated by panel method based program. Developed design process through this study, will be apply to rotorcraft optimum configuration design.