(The) study on anti-inflammatory effect of Cynara scolymus L. (artichoke)

부경준 Sahmyook University 2020 국내석사

동축 전기방사를 이용한 Core/Sheath 구조의 자성 나노섬유 제조 및 특성평가

정다영 서울대학교 대학원 2014 국내석사

MWNTs/Fe3O4 nanocomposites were prepared simply by the coprecipitation method from the solution of ferrous/ferric mixed salt in the presence of MWNTs. The morphology of MWNTs/Fe3O4 nanocomposites was analyzed by HR-TEM, and then the structure and chemical bonding of MWNTs/Fe3O4 nanocomposites were confirmed by XRD and FT-IR analysis. The M-H hysteresis of MWNTs/Fe3O4 nanocomposites shows the superparamagnetic behavior with small remnant magnetization. Magnetorheological fluid was prepared from MWNTs/Fe3O4 nanocomposites dispersed in a mineral oil. The viscosity of MRF increases under magnetic field because the applied field aligns magnetic particles into fibrous structure. Due to the low density of MWNTs, the sedimentation stability of MRF is also improved compared to the MRF based on pure magnetite particles. Field responsive nanofibers with core(MRF)/sheath (polyurethane) structure were fabricated by coaxial electrospinning and the mechanical properties of both with and without magnetic field were measured by tensile tests. Since the load-carrying capacity of MWNTs is outstanding, the tensile stress and the modulus of core/sheath nanowebs are much higher than those of PU nanowebs. When the magnetic fields are applied to core/sheath nanowebs, the mechanical properties improve, but the elongation at break is slightly decreases. Due to the ability to absorb electromagnetic waves, nanowebs containing MWNTs/Fe3O4 nanocomposites can be applied as EMI shielding material. Even in a narrow range of frequencies, core/sheath nanowebs show outstanding EMI shielding properties.

Electro-responsive characteristics of carbon nanomaterials with inorganic coating for smart composites

오세영 서울대학교 2015 국내박사

The inorganic-coated carbon nanomaterials with anisotropic core-shell structure were fabricated for electrorheological (ER) fluid as well as the fiber webs encapsulating ER fluid were produced via a coaxial electrospinning technique for instantly hardening material. First, Multi-walled carbon nanotubes (MWNTs) were used to the template for the fabrication of one dimensional nanomaterials with adequate electrical conductivity (10-7~10-9 S/cm) and high elastic modulus. Silica-coated MWNTs (S-MWNTs) and titania-coated MWNTs (T-MWNTs) with cylindrical structure were synthesized to investigate the ER effects of inorganic materials with different dielectric constant. The electrorheological and dielectric properties of the two different inorganic-coated MWNTs ER fluids were measured using the rheometer and impedance analyzer. The T-MWNTs ER fluids have shown higher dynamic yield stress as well as viscoelastic properties compared to the S-MWNTs ER fluids under external electric field. Furthermore, the T-MWNTs ER fluids have shown higher permittivity and shorter relaxation time of interfacial polarization than those of S-MWNTs. It was caused by intrinsic properties of different inorganic materials which used as shell material. Second, we prepared S-MWNTs with three different shell thickness and inorganic-coated MWNTs with double layer to modify dielectric properties and electrical conductivity of MWNTs. The electrical conductivity of S-MWNTs decreased with an increase of the shell thickness. However, the dynamic yield stress and viscoelastic properties of S-MWNTs suspension increased with an increase the shell thickness and then decreased to the shell thickness 40 nm. This indicates that the electrical conductivity of particles is an important parameter in the ER performance. In the case of ER fluids with double layer, the dynamic yield stress of silica-titania-coated MWNTs (ST-MWNTs) suspension was higher compared to titania-silica-coated MWNTs (TS-MWNTs) suspension at the same volume fraction. It has been found that the outermost shell material plays an important role in the ER performance. Third, titania-coated graphene sheets (TCGSs) were synthesized by a sol-gel method to investigate influence of the difference between 1D and 2D structure and the mixture of T-MWNTs and TCGSs fluids. The value of TCGSs suspension was 2.11, which is similar to the T-MWNTs based ER fluids (2.17). However, the relaxation time of T-MWNTs is faster than that of TCGSs. This is caused by the degree of entanglement and specific surface area between T-MWNTs and TCGSs. Furthermore, mixture effect of T-MWNTs and TCGSs suspension on ER and dielectric properties has been analyzed. The mixture suspension showed the good stability of standing, the enhanced ER performance due to a unique interconnected nanostructure with high contact points and a large surface area, which shows a synergic effect and improves the interfacial interaction between inter-particles and conducting 3D network. Finally, fiber mats composed inorganic-coated MWNTs ER fluids as core part and poly(ethylene terephthalate) (PET) as shell component were produced using co-axial electrospinning technique for instantly hardening textile. The effect of core particle concentration and external field strength on mechanical properties of fiber mats was investigated. The modulus and tensile strength of the fiber mats is enhanced as well as the breaking elongation is reduced under the external electric field due to the increase of fibrillation number in core part.

오발부민으로 유도된 천식동물모델을 이용한 리팜피신의 천식 치료 효능 연구

박준우 삼육대학교 대학원 2019 국내석사

천식은 대표적인 염증성, 폐쇄성 폐 질환으로 현재까지 천식에 대한 확실한 치료제가 없는 실정이다. 본 연구에서는 대표적인 결핵치료제로 알려져 있는 리팜피신의 in vivo 천식 치료 효능을 연구하였다. 오발부민(ovalbumin)에 의해 유도된 천식 마우스 모델을 확립하였고, 각 실험군 별로 마우스의 기관지폐포세척액(BALF)을 분리한 후 BALF 내 염증세포의 수와 TNF- α, IL-6, IL-1β의 양을 측정하였다. trypan blue exclusion 방법과 FACS를 이용하여 BALF에 침윤된 염증세포의 수를 측정한 결과, 천식이 유도된 마우스의 BALF에서 염증세포의 수가 많았으나 천식이 유도된 마우스에 리팜피신을 투여했을 때 염증세포는 낮은 수치를 보여주었다. 또한 천식유도군에 비해 리팜피신 투여군의 BALF내 사이토카인은 낮은 수치를 보여 주였으며, 양성대조군인 덱사메타손(dexamethasone) 처치군과 유사한 효과를 보였다. 이런 결과들은 리팜피신이 염증을 조절함으로써 천식의 증상을 완화시킬 수 있음을 보여준다.

망초와 북시호 혼합추출물의 항염증 효능 연구

김유리 삼육대학교 대학원 2020 국내석사

본 연구에서는 대식세포인 RAW264.7 세포를 사용하여 예로부터 염증 억제, 피부 재생 효능이 있는 것으로 알려진 망초, 복시호 혼합 추출물의 항염증 효능 및 그 기전을 규명하고 염증 치료 물질로서의 가능성을 확인하였다. 먼저 망초, 북시호 혼합물은 속슬렛(Soxhlet) 추출과 동결 건조 과정을 거쳐 본 연구에 사용할 시료를 준비하였고 이를 SYUEB라고 명명하였다. SYUEB의 세포 독성을 확인하기 위하여 RAW264.7 세포를 배양 한 다음, MTT assay를 실행하였다. 그리고 폴리페놀 함량과, 항산화 능력을 측정하였으며, lipopolysaccharide (LPS)로 자극받은 대식세포에서의 Nitric Oxide (NO) 생성 및 iNOS 유전자 발현을 측정하였다. 또한 ELISA, RT-PCR 방법을 통해 염증성 사이토카인의 발현을 측정함으로써 SYUEB의 항염증 효과를 분석하였다. 그 결과 SYUEB는 사용한 농도에서 세포 생존율에 영향을 미치지 않았으며, 폴리페놀함량은 높은 수준으로 측정되었고, 항산화 활성은 양성대조군 Vitamin. C 보다 월등히 높았다. NO 생성능 실험 결과, 활성화된 대식세포에 SYUEB을 처리하였을 때, 세포 활성으로 증가된 NO 생성을 감소시키는 것을 확인 할 수 있었다. 그리고 ELISA, RT-PCR 분석 결과, 활성화된 대식세포에 SYUEB을 처리하였을 때, 염증 매개 인자인 Tumor Necrosis Factor-α(TNF-α), Interluekin-6(IL-6), Interluekin-1β(IL-1β) 발현을 감소시켰다. 따라서 본 연구의 결과, 망초와 북시호 혼합 추출물인 SYUEB가 항염증 효능을 가지고 있음을 확인할 수 있었다.

The study on anti-inflammatory effect of cynara scolymus L. (Artichoke)

부경준 삼육대학교 대학원 2019 국내석사

Artichoke (Cynara Scolymus L.) has been traditionally used in the treatment of digestive-related disease, severe hyperlipidemia and liver disease. Recently, anti-inflammatory effect of artichoke has been shown by several studies, but its mechanisms are still unclear. In the present study, to demonstrate the anti-inflammatory mechanisms of artichoke, we herein examined their effects on the expression of inflammatory mediators. The activation of NF-κB and caspase-1 proteins was also investigated. The expression of inflammatory mediators such as NO, PGE2, TNF-α, IL-1β, and IL-6 were investigated by using ELISA and RT-PCR method in murine macrophage cell line, RAW264.7 cells, stimulated with lipopolysaccharide (LPS). Artichoke inhibited the production of NO, PGE2, IL-1β, IL-6, and TNF-α in macrophages stimulated with LPS in a dose dependent manner. Artichoke also suppressed the mRNA expression of iNOS, COX-2, IL-1β, IL-6, and TNF-α in macrophages stimulated with LPS. Particularly, compared with other mediators, IL-1β expression was dramatically inhibited by artichoke treatment. Therefore, we examined the effect of artichoke on the activation of caspase-1, one of inflammasome complex, which matures the inactive form of IL-1β to active form. Artichoke also suppressed the caspase-1 activity in the cells stimulated with LPS, suggesting that artichoke decreases production of active IL-1β by inhibiting caspase-1. These findings support that artichoke could be a potential therapeutic candidate for the treatment of inflammatory diseases.

Anti-atopic dermatitis effect of scolopendrasin I from Scolopendra subspinipes mutilans

이기만 Sahmyook University 2014 국내박사

The role of Per2, one of circadian genes, in Atopic dermatitis

Prakash Annamneedi V 삼육대학교 2021 국내박사

Every organism has circadian rhythms which are generated from SCN and helps to the organism to know time of day in order to anticipate and respond to changes in the outer environment. Inflammation is a primary response of body for infection, injury or foreign bodies offense and it is critical for both innate and adaptive immunity. Inflammation disrupted the circadian rhythms results these are promoting inflammatory diseases (Cavadini et al., 2007; Kwak et al., 2008; Lundkvist et al., 2002). Per2 is core circadian gene to generate circadian rhythms and it is associated with enhancing inflammation and inflammatory diseases. Circadian clock disruption in immune cells may promote higher levels of inflammatory cytokines. These abnormal circadian rhythms effect on sleep cycle and it is one of the cause of Atopic dermatitis. In this study we investigated the Per2 role in Atopic dermatitis by establishing Per2-/- DNCB (2,4-Dinitrochlorobenzene) mice and siper2 transfected RAW 264.7 cells. We focused on the Per2 gene involvement in Atopic dermatitis (AD) pathogenesis and macrophage inflammation. And Per2 effect on anti-inflammatory clock gene Brain and muscle ARNT-like 1 (BMAL-1) and Th2 transcription factor GATA-binding protein 3 (GATA3). The Clock gene Per2 has an important role in inflammation and it is enhancing inflammatory cytokines. Atopic dermatitis (AD) is a chronic inflammatory skin disorder associated with defective skin barrier. AD is also a Th2 mediated allergic skin disease. In this study we investigated the relation of Per2 with BMAL1(Brain and muscle Arnt-like protein-1) and the role of Per2 in Atopic dermatitis by animal model and inflammation in macrophages in vitro. We established the atopic dermatitis animal model in Per2-/- and Per2+/+ (wild-type) by treatment with 2,4-dinitrochlorobenzene (DNCB). A subset of mice in both Per2-/- and Per2+/+ were treated with saline as control group. It seems that Per2 in wild type mice enhances the Th2 cell differentiation by upregulation of GATA3 (GATA Binding Protein 3) by suppressing BMAL1. Per2-/- mice doesn’t get atopic dermatitis and it is similar like wild type and Per2-/- control groups. The skin severity score was shown mild and Th2 cytokines also too low in AD-induced Per2-/- group. We found that there is a major difference in enhancement of Th2 cytokine levels during AD development between Per2+/+ and Per2-/- mice. Serum IgE was less produced in Per2-/- mice. According these results, we hypothesized Per2 influence to promote atopic dermatitis by inflammation in Per2+/+ mice and Per2-/- mice protected from allergic atopic dermatitis by increasing anti-inflammatory clock gene BMAL1 through supressing GATA3. For in vitro study of Per2 role in RAW 264.7 cell line, we transfected the RAW264.7 cell line with SiRNA by gene silencing. Transfected RAW 264.7 cells doesn’t get inflammatory response while stimulated by LPS. And nitric oxide and inflammatory cytokine production also very low in SiPer2 RAW 264.7 cells than RAW 264.7 cells. We hypothesized that inflammation disrupt and increase Per2 gene levels which results to develops the Atopic dermatitis by suppressing BMAL-1 and inflammation in macrophages. Per2 is playing key role to triggering atopic dermatitis and inflammation in macrophages. Key words: Per2, atopic dermatitis, inflammation.

BALB/c 마우스 모델을 이용한 아티초크의 항천식 효과 연구

배재필 삼육대학교 대학원 2023 국내석사

천식은 천명음, 호흡곤란, 기침 등의 증상이 나타나는 호흡기계의 만성 염증 질환이다. 아티초크(artichoke, Cynara Cardunculus var. scolymus L)는 지중해 연안이 원산지인 쌍떡잎식물이다. 아티초크는 전통적으로 소화기 질환, 고지혈증, 간 질환 및 항산화 연구에 널리 사용되어 왔다. 그러나 천식 치료 효능은 잘 알려져 있지 않았다. 본 연구에서는 동물모델을 이용하여 아티초크의 항천식 효과를 확인하였다. BALB/C 마우스에 오발부민과 알루미늄 하이드록사이드의 혼합물을 사용하여 천식을 유발한 후 아티초크를 농도별로 경구 투여했다. FACS분석을 통해 아티초크를 처리하지 않은 마우스와 비교하여 처리한 마우스의 기관지폐포세척액(BALF)에서 총 세포의 수가 처리되지 않은 마우스에 비해 감소된 것을 확인하였다. 혈장 내 IgE 농도 역시 아티초크를 처치한 실험군에서 감소하였다. TNF-α, IL-6, IL-1β 와 같은 전염증성 사이토카인(pro-inflammatory cytokine)의 농도가 아티초크로 치료한 마우스의 BALF에서 감소했으며, 천식에서 증가하는 특정 사이토카인인 IL-4, IL-5, IL-13의 농도도 아티초크를 처치한 실험군의 BALF 에서 감소했다. 이러한 연구 결과는 아티초크가 항천식 활성을 갖고 있어 천식 질환 치료제 개발에 적용될 수 있음을 시사한다.

Compound 48/80에 의해 자극된 사람 비만 세포주의 염증매개인자 분비에 대한 리팜피신의 억제 효능

김승현 삼육대학교 대학원 2014 국내석사

Allergic disease, such as atopic dermatitis (AD) which is a common inflammatory skin disorder, is mediated by mast cell. Rifampicin is a major drug for treatment of tuberculosis. Recently it was reported that rifampicin has anti-inflammatory activity. This study was performed to determine if rifampicin has anti-allergic activity via the suppression of mast cell activation. The inhibitory effects of rifampicin on human mast cells-1(HMC-1) activation were evaluated by measuring the release of granules and histamine. Rifampicin suppressed the release of β-hexosaminidase and histamine from HMC-1 cells activated with compound 48/80. Anti-inflammatory effect of rifampicin was evaluated using in vitro acute inflammation model. Treatment of rifampicin inhibited secretion of inflammatory mediators, such TNF-α and PGD2, in mast cells stimulated with compound 48/80. The expression of COX-2 was also measured and rifampicin reduced the COX-2 mRNA expression at a dose-dependent manner. These results suggest that rifampicin can be used to treat allergic disorder via the inhibition of histamine release and COX-2 expression. Keywords: rifampicin, mast cell, histamine, COX-2