H-Y 抗體의 種間交叉反應 및 H-Y에 對한 單一클론抗體의 生産에 關한 硏究 = Studies on Interspecific Cross Reaction of H-Y Antibody and Production of Monoclonal Antibodies to H-Y

다국어 초록 (Multilingual Abstract)

These experiments were carried out to investigate cross reaction of H-Y antibody and to produce monoclonal antibodies to H-Y.
H-Y antiserum was prepared in inbred SD female rats by repeated immunization of newborn testis homogenate from same strain. H-Y antibody in antiserum was detected by ELISA. To confirm cross reaction to H-Y antibody, mouse and rabbit embryos were cultured in medium with H-Y antiserum and complement.
After immunization of BALB/c TS to females of same strain, B lymphocytes from mouse producing polyclonal H-Y antibody were fused with myeloma cells. To identify clones producing monoclonal H-Y antibody, ELISA and indirect immunofluorescence test were used.
Results obtained in these experiments were summarized as follows:
1. Following culture of 248 mouse embryos and 42 rabbit embryos in medium containing rat H-Y antibody and complement, 115 (46.4%) mouse embryos and 23 (54.8%) rabbit embryos were arrested or destroyed and cross reaction of H-Y antibody could be confirmed from these results.
2. Of total 384 wells, 80 hybridoma colonies were formed after fusion. Fusion efficiency was 20.8%.
3. Three strong positive wells selected from fused cells by ELISA and 2 wells from these were also confirmed positive by indirect immunofluorescence test.
4. Twelve wells of ELISA-positive were selected after cloning and 2D45D4 clones from these were confirmed to produce monoclonal antibodies to H-Y by indirect immunofluorescence test.
5. Following affinity charomatography on protein A, monoclonal antibodies from 2D45D4 clones were classified immunoglobulin G and concentration of monoclonal antibodies in mouse ascitic fluid was 1mg/ml.

목차 (Table of Contents)

• 目次
• ABSTRACT = i
• Ⅰ. 緖論 = 1
• Ⅱ. 材料 및 方法 = 3
• 1. 試驗材料 = 3
• 2. 試驗方法 = 6
• Ⅲ. 結果 및 考察 = 12
• 1. H-Y抗體의 種間交叉反應 = 12
• 2. H-Y에 대한 單一클론抗體의 生産 = 15
• Ⅳ. 摘要 = 24
• REFERENCES = 26