황칠나무 추출물의 항산화 효과 및 Streptozotocin 유발 당뇨 흰쥐의 항당뇨 효과에 미치는 영향 = Antidiabetic and Antioxidative Effects of Dendropanax morbifera in Streptozotocin -Induced Diabetic Rats

다국어 초록 (Multilingual Abstract)

This study was performed to investigate the antidiabetic effects of leaves and stems in the Dendropanax morifera Leveille (DML) hot Water extracts and ethanol extracts in the diabetic rats. Diabetic experimental model was established by intraperitoneal injection of streptozoticin (STZ) into male ICR mice. DML hot Water and ethnol extracts were concentration of 100 mg/kg and 200 mg/kg were administered orally in diabetic rats. Body weight, blood glucose levels were recorded for 14 days and serum insulin levels and urinalysis and histopathological observation of pancreatic islets were measured at the day 14. oral administration of DML extracts reduced significantly body weight in DW-200 groups. The blood level of glucose was decreased in the dose-dependent manner than DC group; 37.3% in the STZ+DW-200 group, 37.3% in the STZ+DE-200 group, 32.4% in the STZ+DW-100 group, and 19.3% in the DE-100 group. The serum level of insulin was improved 1.9 folds in the STZ+DW-200 group and 2.3 folds in the STZ+DE-200 group compared to the DC group. The urine level of glucose was improved 3.8 folds in the STZ+DW-200 group and 11.4 folds in the STZ+DE-200 group compared to the DC group. Immunohistochemical stainig for insulin clearly showed that pretreatment with DW-200 group and DE-200 group blocked the STZ-induced islets destruction and restored the number of islet cells that secreted insulin to the level of the control. The contents of total polyphenol and total flavonoids in 1g dry mass of DML extracts(DLW, DML leaves hot Water extracts; DLE, DML leaves ethanol extracts; DSW, DML stems hot Water extracts; DSE, DML stems ethanol extracts) were DLW (19080.51㎍), DLE (12561.03㎍), DSW (12207.18㎍), DSE (10266.66㎍) of gallic acid equivalents and DLW (1018.90㎍), DLE (803.79㎍), DSW (228.83㎍), DSE (220.68㎍) of quercetin equivalents, respectively. In addition, the antioxidant and DPPH radical scavenging activity of DML extracts IC50 were DLE (243.33㎍/㎖), DLW (212.70㎍/㎖), DSW (308.30㎍/㎖), DSE (317.07㎍/㎖). These results imply the intake of DML hot Water extracts could contribute to attenuate clinical symptoms of diabetes mellitus and improves β-cell proliferation and prevents the death of β-cells in STZ-induced diabetic rats.

목차 (Table of Contents)

• 차 례
• Ⅰ. 서 론 1
• Ⅱ. 재료 및 방법 6
• 1. 재료 6
• 1) 시료 6
• 2) 열수추출물의 제조 6
• 3) 에탄올추출물의 제조 7
• 2. In vitro 항산화 효과 7
• 1) 1,1-Diphenyl-2-picrylhydrazyl(DPPH) radical소거능 7
• 2) Total polyphenol 8
• 3) Total flavonoid 8
• 3. In vivo 항당뇨 효과 9
• 1) 실험동물 및 사육 9
• 2) 실험 디자인 9
• 3) 체중 및 혈당 변화 측정 10
• 4) 혈액 및 췌장 채취 10
• 5) 뇨 분석 11
• 6) 혈중 인슐린 농도 측정 11
• 7) 췌장 조직 처리 및 세포구축학적 변화 12
• 8) 면역조직화학적 염색 13
• 4. 자료처리 및 분석 14
• Ⅲ. 연구결과 15
• 1. 황칠나무 추출물의 In vitro 항산화 효과 15
• 1) DPPH radical 소거능 15
• 2) Total polyphenol 18
• 3) Total flavonoid 18
• 2. 황칠나무 추출물의 In vivo 항당뇨 효과 21
• 1) 체중의 변화 21
• 2) 혈당의 변화 23
• 3) 뇨 분석 26
• 4) 혈장 insulin 농도 28
• 5) H&E 및 인슐린에 대한 면역조직학적 변화 30
• Ⅳ. 요약 및 결론 35
• 참고문헌 38
• Abstract 43