Oryzanol과 Ferulic acid 보충이 지질대사와 제2형 당뇨대사 개선에 미치는 영향 = Influence of oryzanol and ferulic acid on the lipid metabolism, glucose metabolism and antioxidative status in high fat-fed mice

다국어 초록 (Multilingual Abstract)

The incidence of obesity, diabetes is rapidly increasing worldwide and the need for natural drugs against the disease has become stronger. The objective of this study was to investigate the effects of oryzanol and ferulic acid on the lipid metabolism and glucose metabolism of high fat-fed mice.
MATERIALS AND METHODS:
Male C57BL/6 mice were randomly divided into 4 groups: NC group fed with normal diet (NC group), HF group fed with high fat (17%) diet, HF-O group fed with high fat diet supplemented with 0.5% oryzanol and HF-FA group fed with high fat diet supplemented with 0.5% ferulic acid. All animals were allowed free access to the experimental diets and water for 7 weeks.
Experiment I: The concentrations of plasma total cholesterol (TC), triglyceride (TG), HDL-cholesterol (HDL-C) and HDL-C/TC ratio were measured. The activities of hepatic lipid-regulating enzymes, such as malic enzyme (ME), fatty acid synthetase (FAS) and Glucose-6-phosphate dehydrogenase (G6PD), were also determined. The antioxidative state was measured by the detection of SOD, GSH-Px, catalse, glutathione reductase (GR), plasma TBARS, and erythrocyte TBARS
Experiment II: The blood glucose, glycogen and insulin levels were measured. The activities of hepatic glucose-regulating enzymes, such as glucokinase (GK), glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), were also determined.
RESULTS:
The HF mice exhibited increased body weight gain, plasma and hepatic total cholesterol and triglyceride concentrations and lipid peroxidation rate, and reduced high-density lipoprotein cholesterol level. In general, they also showed lower hepatic antioxidant and higher lipid-regulating enzymes activities relative to those of NC group. Addition of oryzanol or ferulic acid in the diet counteracted these high fat-induced hyperlipidemia and oxidative stress
via increased fecal lipid excretion and regulation of antioxidant and lipogenic enzyme sactivities.
The HF-O and HF-FA groups exhibited significantly lower blood glucose level and G6pase and PEPCK activities and higher glycogen and insulin concentrations and GK activity compared with NC and HF groups.
DISCUSSION:
Results of this study illustrate that oryzanol and ferulic acid have relatively similar hypolipidemic actions and could be effective in lowering the risk of high fat diet-induced obesity. They also suggested that both oryzanol and ferulic acid could reduce the risk of high fat diet-induced hyperglycemia via regulation of insulin secretion and hepatic glucose-regulating enzyme activities.

목차 (Table of Contents)

• I. 서 론 1
• 1. 연구 목적 1
• 2. 연구 배경 5
• 2-1. 미강에 함유된 성분들의 생리활성 5
• 2-2. 대사증후군 관련 체내 대사들의 조절 기전 8
• II. 실험재료 및 방법 26
• 1. 실험 재료 및 실험 식이 26
• 2. 채혈 및 조직의 채취 29
• 3. 생화학적 분석 29
• 3-1. 혈장 지질농도 분석 29
• 3-2. 간조직 중성지질 및 콜레스테롤 함량 측정 30
• 3-3. 분변 중 지질함량 측정 31
• 3-4. 간조직의 효소원 분리 및 단백질 정량 31
• 3-5. 항산화관련 효소 활성도 측정 32
• 3-6. 지질 대사 관련 효소 활성도 측정 35
• 3-7. 당대사효소 활성도 측정 37
• 3-8. 글리코겐 함량 측정 38
• 3-9. 인슐린함량 측정 38
• 3-10. 혈당 측정 39
• III. 결 과 40
• 1. 체중 증가량 및 장기무게에 미치는 영향 40
• 1-1. 체중증가량비교 40
• 1-2. 장기중량 비교 41
• 2. 혈장 지질 농도에 미치는 영향 42
• 3. 간조직의 지질함량에 미치는 영향 45
• 4. 분변 중 지질함량에 미치는 영향 47
• 5. 간조직의 지질대사 효소 활성도에 미치는 영향 49
• 6. 간조직 항산화 효소 활성도에 미치는 영향 51
• 7. 간조직의 paraoxonase 활성도 비교 54
• 8. 혈장,적혈구 과산화지질 수준에 미치는 영향 54
• 9. 혈당량에 미치는 영향 56
• 10. glycogen 함량과의 관계 57
• 11. Insulin 농도와의 관계 58
• 12. 당질대사 관련 효소에 미치는 영향 59
• IV. 고 찰 62
• 1. oryzanol, ferluic acid 보충에 의한 비만 및 지질대사 관련성 연구 62
• 2. oryzanol, ferulic acid 보충과 항산화효소의 활성과의 관련성 연구 67
• 3. oryzanol, ferulic acid 보충의 당대사 질환 개선작용 연구 69
• V. 요약, 결론 73
• VI. 참고문헌 77
• VII. Abstract 88