桑白皮의 선천면역 활성화에 의한 항암 효과 = Anti-tumor metastatic effect and activation of innate immunity by extract of Mori Radicis Cortex

다국어 초록 (Multilingual Abstract)

Objective: This study was carried out to investigate the anti-tumor metastasis effect and activation of innate immunity by extracts of Mori radicis cortex.
Methods: Anti-tumor metastatic experiment was conducted in vitro and in vivo by using colon 26-M3.1 carcinoma cell, L5178Y-R lymphoma cell and Hela cell. To observe the activation of innate immunity by extracts of Mori radicis cortex, we estimated IL-6, IL-10, IL-12, TNF-α from peritoneal macrophages. And we evaluated the activation of NK cell by using anti-asialo-GM1 serum.
Results: We found that the administration of Mori radicis cortex extracts significantly inhibited tumor metastasis. In an in vitro cytotoxicity analysis, Mori radicis cortex affected tumor cell growth above specific concentration. Mori radicis cortex also stimulated peritoneal macrophage, which was followed by the production of various cytokines such as IL-6, IL-10, IL-12, TNF-α. The depletion of NK cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Mori radicis cortex on tumor metastasis.
Conclusion: Mori radicis cortex appears to have considerable activity on the anti-metastasis by activation of innate immunity.

목차 (Table of Contents)

• Ⅰ. 緖論 = 1
• Ⅱ. 實驗 = 2
• 1. 材料 = 2
• 1) 藥材 = 2
• 2) 動物 = 2
• 2. 方法 = 2
• 1) 試藥 = 2
• 2) 細胞培養 = 3
• 3) 腫瘍 轉移 모델 = 3
• 4) 腫瘍細胞에 대한 細胞毒性 = 3
• 5) Macrophage에 대한 影響 = 4
• (1) Macrophage의 收集 = 4
• (2) Macrophage에 대한 細胞毒性 = 4
• (3) Macrophage의 cytokines 測定 = 4
• 6) NK cell 關聯 腫瘍 轉移 모델 = 5
• 7) 統計處理 = 5
• Ⅲ. 結果 = 6
• 1. 桑白皮 추출물 정맥투여 후 종양세포 전이 억제 = 6
• 2. 腫瘍 細胞에 대한 細胞毒性 影響 = 7
• (1) Colon26-M3.1 carcinoma cell에 대한 細胞毒性 效果 = 7
• (2) L5178Y-R lymphoma cell에 대한 細胞毒性 效果 = 8
• (3) Hela cell에 대한 細胞毒性 效果 = 9
• 3. Macrophage의 cytokine 분비에 미치는 影響 = 10
• (1) Macrophage에 대한 細胞毒性 效果 = 10
• (2) IL-6의 분비 = 11
• (3) IL-10의 분비 = 12
• (4) IL-12의 분비 = 13
• (5) TNF-α의 분비 = 14
• 4. NK cell 유무에 따른 종양 전이 억제 = 15
• Ⅳ. 考察 = 16
• Ⅴ. 結論 = 20
• Ⅵ. 參考文獻 = 21
• Abstract = 24