Shrimp appears to be an alternative fishery resource because of the reduction of fish resource, which represents the primary catching of fish around the Yellow and Southern Seas in Korea, and particularly, the share of big size oriental shrimp, medium...
Shrimp appears to be an alternative fishery resource because of the reduction of fish resource, which represents the primary catching of fish around the Yellow and Southern Seas in Korea, and particularly, the share of big size oriental shrimp, medium size shiba shrimp and spotted shrimp catches takes over 50% of total shrimp catch. Meantime, the natural odor of crustacea such as shrimp does spread an unique flavor which is quite different from those of mammalian meats. When stored for a long time, the unique odor is to fade away stinking with strong fish odor and off-flavor due to its decreased freshness.
This study intends to develop a shrimp reaction flavor which is capable of enhancing the favorite taste sensually by masking the fishy odor and off-flavor and by reinforcing the unique flavor of shrimp through Maillard reaction process using the shrimp hydrolysate and reactive precursors. In this study, the objectives are to optimize the enzymatic hydrolysis condition of shrimp as the pre-treatment process for shrimp reaction flavor production; to determine optimum precursors and reacting condition, which remove fish odor of the seafood inherent but further reinforce good shrimp flavor; finally to develop boiled-type shrimp reaction flavor used in the seafood sauces and fish meat paste and grilled-type reaction shrimp flavor used in the production of snack and seasoning. In order to obtain the optimum hydrolysis condition of spotted shrimp by the type of enzymes, the two independent variables of reaction pH and temperature are selected based on the central composite design system designating the hydrolysis and anti oxidation efficiencies as dependent variables by using a response surface methodology. The optimum hydrolysis conditions for proteolytic enzymes such as Alcalase, Flavourzyme, Protamax and Nutrase were pH 9.3 and 65.2℃, pH 4.1 and 54.5℃, pH 6.6 and 42.5℃, pH 6.1 and 45.2℃, respectively. Among the four enzymes, Alcalase showed the best anti oxidative activities of 24.9% DPPH-scavenging activity and 26.9% Fe-chelating activity.
Depending on the application purpose, boiled-type shrimp reaction flavor of producing the boiled flavor used in instant noodle soup and seafood sauce, and grilled-type shrimp reaction flavor used in the flavoring, snacks and cookies are manufactured. To manufacture boiled-type shrimp reaction flavor, methionine and sucrose are selected as reaction precursors designating the reactive manufacturing condition as pH 7.5 and 1hr reaction time. To manufacture grilled-type shrimp reaction flavor, methionine, threonine, glucosamine and xylose were selected as reaction precursors designating the reactive manufacturing condition as pH 7.5 and 2hr reaction time.
Upon executing the analysis on the volatile contents of sulfides and pyrazines which are presumed to further reinforce the unique shrimp flavor, no sulfides and pyrazines are identified from the hydrolyzate. From the boiled-type shrimp reaction flavor, two sulfur-containing compounds of dimethyl disulfide and dimethyl trisulfide and two nitrogen-containing compounds of 2,5-dimethyl pyrazine and 2,3-dimethyl pyrazine were identified. From the grilled-type shrimp reaction flavor, dimethyl disulfide and 7 pyrazines of pyrazine, methyl pyrazine, 2,5-dimethyl pyrazine, 2,3-dimethyl pyrazine, 2-ethyl-5-methyl pyrazine, trimethyl pyrazine and tetramethyl pyrazine were identified.
The boiled-type and grilled-type shrimp reaction flavors were made in the shapes of extracts and powders with the different mixing ingredients for pure type and seasoning type. The extracts were dried by vacuum drying, spray drying and freeze drying. The powders of grilled-type did show no sensory difference in terms of drying methods. As well, the comprehensive sensory score of seasoning type proved to be better than the pure type.