동위효소 및 RAPD분석에 의한 한국재래종 누에계통의 계통학적 특성 = Phylogenetic Relationships and Characterization of Korean Native Silkworm Strains Based on RAPDs and Isozyme Analysis, Bombyx mori

국문 초록 (Abstract)

집누에는 지리적인 특징에 의해 중국종, 일본종, 유럽종, 열대종 및 한국종으로 분류된다. 한국종계통은 품종수도 적을 뿐만 아니라 관련 연구도 미미한 실정이다. 본 연구는 한국재래종으로 추측되는 품종들을 국내 ? 외로부터 수집하여 동위효소 및 체액단백지유전자와 RAPD 다형분석을 실시하여 한국재래종 계통의 품종적 유연관계와 계통특성을 밝히고자 하였다.
1. 동위효소유전자의 분석결과, 몇 개의 유전자군에서 한국재래종과 타 지역종간에 유전자형 및 유전자 빈도의 차이가 명확히 나타났다.
2. RAPD의 결과를 UPGMA법에 의해 분석한 결과, 집누에군과 멧누에군으로 크게 구분되었으며 유전적 유사계수 0.6930을 기준으로 한국재래종, 일본종 및 중국종으로 그룹화되었다.
3. 이상의 결과를 종합하면, 한국재래종 누에계통은 하나의 지역종 원종계통으로 분류될 수 있는 명확한 유전적 특성을 가지는 것은 물론 한국종의 계통특성도 뚜렷한 것으로 확인되었다.

다국어 초록 (Multilingual Abstract)

This study was conducted to elucidate phylogenetic relationships and genetic characterization of silkworms that might be recohnized as the Korean native strains. Genetic characterization in isozymes and the proteins of larval hemolymph of 17 silkworms were observed by acrylamide gel eletrophoresis, on 12 genes; Bph, Bes, Ies, Amy-hc, Ict-A, -B,-D, -E -H,Pfl, Pst, Lp. Gene frequencies in each locus were compared other geographic strains. Korean native strains were remarkably different from others considered as the genetic characterization of Korean native strains. Phylogenetic relationships in Korean native strains were analysed using RAPD-PCR markers. A total of 40 primers were used and 346 bands of amplified DNA were generated from geographic strains, Genetic similarity based on the RAPD bands was used to construct phylogenetic dendrogram based on analysis of band sharing data of amplified markers. Genetic similarity ranged from 0.595 to 0.860. In the genetic relationship based on dendrogram, they were classified into Bombyxmori group (including 16 domesticated silkworm strains) and B. mandarina group. The Bombyx mori group was separated into three sub-groups at the genetic similarity of 0.6930, including Korean, Japanese and Chinese groups. According to this result, the Korean native variety can be considered as a clearly different variety from other geographic strains. It may be concluded that the Korean native strains are also one of original geographic rariety such as Japanese, Chinese, etc.