L-Carnitine, pyridoxine hydrochloride, DL-α-tocopheryl acetate를 이용한 분유모델시스템의 마이얄반응생성물 저감화 조건 최적화

국문 초록 (Abstract)

본 연구는 분유모델시스템에 L-carnitine, pyridoxine hydrochloride, DL-α-tocopheryl acetate를 첨가하여 Maillard 반응에 의해 생성된 MRPs를 저감화 시키기 위한 최적조건을 찾기 위해 RSM의 CCD를 이용하였다. L-Carnitine (X₁), pyridoxine hydrochloride (X₂), DL-α-tocopheryl acetate (X₃)의 농도를 독립변수로 하고 형광도와 HMF 함량을 종속변수로 각각 설정하였다. 종속변수 회귀식의 결정계수(R<SUP>2</SUP>)는 각각 0.942, 0.861로 반응표면분석 모델에 적합하였다. 형광도와 HMF 함량은 L-carnitine과 pyridoxine hydrochloride의 농도가 낮을 때 DL-α-tocopheryl acetate의 농도가 감소할수록 그 값이 급격히 감소하였다. L-Carnitine의 농도가 높을 때 pyridoxine hydrochloride의 농도가 20 μM 이하로 감소할수록 형광도가 감소하였고 HMF 함량은 L-carnitine의 농도에 관계없이 pyridoxine hydrochloride의 농도가 20 μM 이하로 감소할수록 감소하는 경향을 나타냈다. 본 실험에서 분유모델시스템에서 생성된 MRPs를 저감화 할 수 있는 최적조건으로 L-carnitine, pyridoxine hydrochloride, DL-α-tocopheryl acetate의 농도는 각각 2.26, 15.77, 20.63 μM이었다. 이때 형광도는 77.4%였고 HMF 함량은 248.7 ppb로 각각 유단백질-유당 마이얄 반응생성물(LC, lactose+sodium caseinate)대비 MRPs를 22.6, 23.1% 감소시킬 수 있다고 예측할 수 있었다. 또한, RSM을 통해 찾은 최적 조건의 실험값으로 형광도는 79.3%였고 HMF 함량은 247.6 ppb로 각각 LC대비 MRPs를 20.7, 17.8% 감소 시켰다. 따라서, L-carnitine, pyridoxine hydrochloride, DL-α-tocopheryl acetate의 최적화된 혼합을 통하여 분유제조 시 MRPs 생성을 저감화 시킬 수 있을 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

The Maillard reaction is a non-enzymatic reaction between amino and carbonyl groups. During milk processing, lactose reacts with milk protein through this reaction. Infant formulas (IFs) are milk-based products processed with heattreatments, including spray-drying and sterilization. Because IFs contain higher Maillard reaction products (MRPs) than breast milk, formula-fed infants are subject to higher MRP exposure than breast milk-fed ones. In this study, we investigated the optimization of conditions for minimal MRP formation with the addition of L-carnitine (L-car), pyridoxine hydrochloride (PH), and DL-α-tocopheryl acetate (α-T) in an IF model system. MRP formation was monitored by response surface methodology using fluorescence intensity (FI) and 5-hydroxymethylfurfural (HMF) content. The optimal condition for minimizing the formation of MRPs was with 2.3 μM L-car, 15.8 μM PH, and 20.6 μM α-T. Under this condition, the predicted values were 77.4% FI and 248.7 ppb HMF.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• References

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