가시아메바(Acanthamoeba spp.)와 파울러자유아메바(Naegleria fowleri)는 자유생활아메바로 자연계에 널리 분포하며 사람과 동물에게 치명적인 질병을 일으킨다. 본 연구에서는 가사아메바와 파울러...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A105447044
김민정 (한국수자원공사) ; 이규철 (한국수자원공사 수자원연구원) ; 김건우 (한국수자원공사 수질연구센터) ; 이현지 (한국수자원공사 수질연구센터) ; 김민영 (한국수자원공사 수질연구센터) ; 서대근 (한국수자원공사) ; 이정엽 (K-water 수질분석연구센터) ; 조영철 (충북대학교) ; Kim, Min-jeong ; Lee, Gyu-Cheol ; Kim, Kunwoo ; Lee, Hyunji ; Kim, Min Young ; Seo, Dae Keun ; Lee, Jeong Yeob ; Cho, Young-Cheol
2018
English
KCI등재,SCOPUS
학술저널
98-104(7쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
가시아메바(Acanthamoeba spp.)와 파울러자유아메바(Naegleria fowleri)는 자유생활아메바로 자연계에 널리 분포하며 사람과 동물에게 치명적인 질병을 일으킨다. 본 연구에서는 가사아메바와 파울러...
가시아메바(Acanthamoeba spp.)와 파울러자유아메바(Naegleria fowleri)는 자유생활아메바로 자연계에 널리 분포하며 사람과 동물에게 치명적인 질병을 일으킨다. 본 연구에서는 가사아메바와 파울러자유아메바를 물 환경에서 조사하기 위해 기존에 보고된 네 종류의 분자생물학적 방법과 상용 real-time PCR 키트의 분석 민감도를 비교하였다. 그 결과 duplex real-time PCR 방법이 민감도가 가장 좋았으며, 동시에 두 종류의 자유생활아메바를 검출할 수 있었다. 따라서 이 방법을 사용하여 한국의 대전시에 위치한 3개 하천, 6개 지점을 대상으로 그 분포를 2회 조사하였다. 가시아메바는 12개 시료 중 10개 시료에서 검출되었으며(83.3%), 파울러자유아메바는 2개 시료에서 검출되었다(16.6%). 향후 이러한 유해 아메바로부터 먹는 물의 안전성을 확보하기 위해 지속적인 분포조사가 필요할 것이다.
다국어 초록 (Multilingual Abstract)
Naegleria fowleri and Acanthamoeba spp. are free-living amoebas that are widely distributed in natural environments. Although uncommon, infection with these protozoans can cause fatal disease in humans and animals. In this study, in order to select th...
Naegleria fowleri and Acanthamoeba spp. are free-living amoebas that are widely distributed in natural environments. Although uncommon, infection with these protozoans can cause fatal disease in humans and animals. In this study, in order to select the appropriate method to survey Naegleria fowleri and Acanthamoeba spp. in water samples, four molecular biology techniques and one commercially available kit for real-time PCR were compared. The results indicated that the duplex real-time PCR was the most sensitive, and could be used to simultaneously detect two different free-living amoebas. Using the duplex real-time PCR approach, the two free-living amoebas were surveyed in three local streams in Daejeon, Republic of Korea. The concentrated free-living amoebas were inoculated onto non-nutrient agar plates which had been spread with heat-inactivated Escherichia coli and incubated for 5~7 days. After incubation, gDNA was extracted and used as the template for amplification by duplex real-time PCR. Acanthamoeba spp. and N. fowleri was detected from ten (83.3%) and two (16.6%) of the twelve samples, respectively. As these two free-living amoebas can be fatal, continuous surveillance is needed to track their distribution in the aquatic environment for the drinking water safety.
참고문헌 (Reference)
1 정은영, "상수원수 및 정수처리공정별 가시아메바 분포특성에 관한 연구" 한국환경과학회 17 (17): 1121-1127, 2008
2 J. M. Schroeder, "Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge" American Society for Microbiology 39 (39): 1903-1911, 2001
3 Shady T. Awwad, "Updates in Acanthamoeba Keratitis" Ovid Technologies (Wolters Kluwer Health) 33 (33): 1-8, 2007
4 정해진, "The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea" 대한기생충학ㆍ열대의학회 43 (43): 1-13, 2005
5 Johan F De Jonckheere, "The impact of man on the occurrence of the pathogenic free-living amoeboflagellate Naegleria fowleri" Future Medicine Ltd 7 (7): 5-7, 2012
6 Denoncourt, A.M., "Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria" 5 : 240-, 2014
7 Govinda S. Visvesvara, "Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris , Naegleria fowleri , and Sappinia diploidea" Oxford University Press (OUP) 50 (50): 1-26, 2007
8 이양진, "Naegleria fowleri Lysate Induces Strong Cytopathic Effects and Pro-inflammatory Cytokine Release in Rat Microglial Cells" 대한기생충학ㆍ열대의학회 49 (49): 285-290, 2011
9 Y. Qvarnstrom, "Multiplex Real-Time PCR Assay for Simultaneous Detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri" American Society for Microbiology 44 (44): 3589-3595, 2006
10 J DEJONCKHEERE, "Molecular Definition and the Ubiquity of Species in the Genus" Elsevier BV 155 (155): 89-103, 2004
1 정은영, "상수원수 및 정수처리공정별 가시아메바 분포특성에 관한 연구" 한국환경과학회 17 (17): 1121-1127, 2008
2 J. M. Schroeder, "Use of Subgenic 18S Ribosomal DNA PCR and Sequencing for Genus and Genotype Identification of Acanthamoebae from Humans with Keratitis and from Sewage Sludge" American Society for Microbiology 39 (39): 1903-1911, 2001
3 Shady T. Awwad, "Updates in Acanthamoeba Keratitis" Ovid Technologies (Wolters Kluwer Health) 33 (33): 1-8, 2007
4 정해진, "The role of domestic tap water in Acanthamoeba contamination in contact lens storage cases in Korea" 대한기생충학ㆍ열대의학회 43 (43): 1-13, 2005
5 Johan F De Jonckheere, "The impact of man on the occurrence of the pathogenic free-living amoeboflagellate Naegleria fowleri" Future Medicine Ltd 7 (7): 5-7, 2012
6 Denoncourt, A.M., "Potential role of bacteria packaging by protozoa in the persistence and transmission of pathogenic bacteria" 5 : 240-, 2014
7 Govinda S. Visvesvara, "Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris , Naegleria fowleri , and Sappinia diploidea" Oxford University Press (OUP) 50 (50): 1-26, 2007
8 이양진, "Naegleria fowleri Lysate Induces Strong Cytopathic Effects and Pro-inflammatory Cytokine Release in Rat Microglial Cells" 대한기생충학ㆍ열대의학회 49 (49): 285-290, 2011
9 Y. Qvarnstrom, "Multiplex Real-Time PCR Assay for Simultaneous Detection of Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri" American Society for Microbiology 44 (44): 3589-3595, 2006
10 J DEJONCKHEERE, "Molecular Definition and the Ubiquity of Species in the Genus" Elsevier BV 155 (155): 89-103, 2004
11 G. Greub, "Microorganisms Resistant to Free-Living Amoebae" American Society for Microbiology 17 (17): 413-433, 2004
12 양혜원, "Loop-Mediated Isothermal Amplification Targeting 18S Ribosomal DNA for Rapid Detection of Acanthamoeba" 대한기생충학ㆍ열대의학회 51 (51): 269-277, 2013
13 Payal Sarkar, "Inactivation of Naegleria Fowleri by chlorine and ultraviolet light" American Water Works Association 104 (104): E173-E180, 2012
14 F. Marciano-Cabral, "Identification of Naegleria fowleri in Domestic Water Sources by Nested PCR" American Society for Microbiology 69 (69): 5864-5869, 2003
15 Augusto Julio Martinez, "Free-living, Amphizoic and Opportunistic Amebas" Wiley 7 (7): 583-598, 1997
16 Frederick L. Schuster, "Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals" Elsevier BV 34 (34): 1001-1027, 2004
17 Vincent Thomas, "Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality" Oxford University Press (OUP) 34 (34): 231-259, 2010
18 Eun-Kyung Moon, "Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions against Acanthamoeba castellanii" 대한기생충학ㆍ열대의학회 54 (54): 697-702, 2016
19 Cursons, R.T, "Effect of disinfectants on pahtogenic free-living amoebae: in axenic conditions" 40 : 62-66, 1980
20 De Jonckheere, J., "Differences in destruction of cysts of pathogenic and nonpathogenic Naegleria and Acanthamoeba by chlorine" 31 : 294-297, 1976
21 da Rocha-Azevedo, B., "Diagnosis of infections caused by pathogenic free-living amoebae" 251406-, 2009
22 Reveiller, F.L., "Development of a nested PCR assay to detect the pathogenic free-living amoeba Naegleria fowleri" 88 : 443-450, 2003
23 Aongart Mahittikorn, "Development of a Rapid, Simple Method for Detecting Naegleria fowleri Visually in Water Samples by Loop-Mediated Isothermal Amplification (LAMP)" Public Library of Science (PLoS) 10 (10): e0120997-, 2015
24 김종현, "Contact-Independent Cell Death of Human Microglial Cells due to Pathogenic Naegleria fowleri Trophozoites" 대한기생충학ㆍ열대의학회 46 (46): 217-221, 2008
25 William D. Mathers, "Confirmation of Confocal Microscopy Diagnosis of Acanthamoeba Keratitis Using Polymerase Chain Reaction Analysis" American Medical Association (AMA) 118 (118): 178-, 2000
26 Derda, M., "Comparative analyses of different genetic markers for the detection of Acanthamoeba spp. isolates" 59 : 472-477, 2014
27 Ashley M. Richards, "Cellular microbiology and molecular ecology of Legionella –amoeba interaction" Informa UK Limited 4 (4): 307-314, 2014
28 Ruqaiyyah Siddiqui, "Biology and pathogenesis of Acanthamoeba" Springer Nature 5 (5): 6-, 2012
29 V. Thomas, "Amoebae in domestic water systems: resistance to disinfection treatments and implication in Legionella persistence" Wiley 97 (97): 950-963, 2004
30 Kyung Il Im, "Acanthamoebiasis in Korea: two new cases with clinical cases review" Yonsei University College of Medicine (KAMJE) 39 (39): 478-, 1998
31 F. Marciano-Cabral, "Acanthamoeba spp. as Agents of Disease in Humans" American Society for Microbiology 16 (16): 273-307, 2003
32 Seal, D.V., "Acanthamoeba keratitis" 309 : 1019-, 1994
33 Lucia Maďarová, "A real-time PCR diagnostic method for detection of Naegleria fowleri" Elsevier BV 126 (126): 37-41, 2010
Draft genome sequence of lytic bacteriophage KP1 infecting bacterial pathogen Klebsiella pneumoniae
Complete genome sequence of Salmonella Thompson strain MFDS1004024 isolated from crab-stick
Draft genome sequence of Streptomyces sp. P3 isolated from potato scab diseased tubers
Complete genome sequence of Flavivirga eckloniae ECD14T isolated from a seaweed Ecklonia cava
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2023 | 평가예정 | 해외DB학술지평가 신청대상 (해외등재 학술지 평가) | |
2020-01-01 | 평가 | 등재학술지 유지 (해외등재 학술지 평가) | |
2013-12-02 | 학술지명변경 | 외국어명 : The Korean Journal of Microbiology -> Korean Journal of Microbiology | |
2010-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2008-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2006-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2001-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
1998-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.21 | 0.21 | 0.21 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.26 | 0.24 | 0.48 | 0.02 |