RH-531을 施用한 大豆의 Globulin 및 Glutelin의 加水分解에 의하여 生成되는 아미노酸의 組成 = The Amino Acid Composition of Globulin and Glutelin in The RH-531 Treared Soybeans

다국어 초록 (Multilingual Abstract)

The crude globulin and glutelin were fractionated from the RH-531 treated soybeans and after the hydrolysis of these proteins by 6N-HCl solution their amino acids were separated and identified by the thin layer chromatography. The results are summarized as follow.
1. The hydrolysis of globulin and glutelin prepared from six kinds of RH-531 treated soybeans offerded the mixtures of 15 kinds of amino acids, which were identified by the thin layer chromatography, respectively, as aspartic acid, lysine, glutamic acid, serine, glycine, arginine, threonine, alanine, histidine, tyrosine, valine, methionine, leucine, proline and phenyl alanine.
2. The yield of crude globulin was highest in sample 3 and except the control the yield of crude glutelin was also highest in sample 3. And the total yield of NaCl-soluble fraction and NaOH-soluble fraction was also highest in sample 3.
3. In the one-dimensional chromatogram, depending on the increase of RH-531 amount, the contents of the globulin-composing amino acids increased, while the contents of glutelin-composing amino acids decreased.
4. In the two dimensional chromatogram the change of the composition of amino acids of globulin and glutelin was hardly observed but, sometimes, methionine and histidine were not detected. On the other hand, the change in the contents of amino acids of these two proteins was observable and was similar to the results reported by another authors.