Bacillus sp. CW - 1121이 생성하는 Alkaline Protease의 생산 및 정제

국문 초록 (Abstract)

Alkaline protease의 생산능력이 강한 Bacillus sp. CW-1121을 토양으로부터 분리 동정하고, 이 균주에 의한 효소생산 조건을 규명한 결과 trypton yeast extract medium에 5일간 배양하였을때 최대의 활성을 나타내었다. 황산암모늄 염석, Sephadex G-150을 사용한 gel filtration과 DEAE-cellulose 이온교환 크로마토그래피를 통하여 이 효소를 정제하여 수율 16.71%, 정제도 5.72배의 효소를 얻었으며, 정제효소의 분자량은 SDS-polyacrylamide 전기영동에 의하여 55,000 정도로 추정된다.

다국어 초록 (Multilingual Abstract)

Alkaline protease producing bacteria were isolated from soil and identified as Bacillus sp. CW-1121. It was found that the production of alkaline protease reached to maximum in 5 day of fermentation at 40℃. The enzyme was purified by ammonium sulfate precipitation, gel filtration on Sephadex G-150 and DEAE-cellulose ion-exchange chromatography. The homogeneity of the purified enzyme was verified by polyacrylamide gel electrophoresis. The enzyme was purified 5.72 fold and yield of the enzyme purification was 16.71%. When the purified enzyme was applied to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight was estimated to be 55,000.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 문헌