An alkaline protease producting bacteria was isolated from soil and identified as Bacillus sp. CW-1121. It was found that the production of alkaline protease reached to maximum when casein, (NH₄)₂HPO₄, MgSO₄, fructose as organic and inorganic ...
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
https://www.riss.kr/link?id=A3291223
이우제 ; 조영제 ; 손규목 ; 최정 ( Woo Je Lee ; Young Je Cho ; Gyu Mok Son ; Cheong Choi )
1991
-
400
SCIE,SCOPUS,KCI등재
학술저널
537-542(6쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
An alkaline protease producting bacteria was isolated from soil and identified as Bacillus sp. CW-1121. It was found that the production of alkaline protease reached to maximum when casein, (NH₄)₂HPO₄, MgSO₄, fructose as organic and inorganic ...
An alkaline protease producting bacteria was isolated from soil and identified as Bacillus sp. CW-1121. It was found that the production of alkaline protease reached to maximum when casein, (NH₄)₂HPO₄, MgSO₄, fructose as organic and inorganic nitrogen, inorganic salt and carbon source added in culture medium. The optimum temperature and pH for the purified enzyme activity were 40℃ and pH 10.0 respectively. The enzyme was relatively stable in the pH range of pH 9.0∼11.0 and at the temperature below 50℃. The activity of the purified enzyme was inhibited by Hg^(2+) and Fe^(2+), whereas Mn^(2+) and K^+ gave rather activating effects on the enzyme activity. PCMB, 2,4-DNP, ε-aminocaproic acid did not show inhibitory effect in the proteolytic activity of alkaline protease but ethylenediaminetetraacetic acid inhibited the enzyme activity. The reaction of this enzyme followed typical Michaels Menten kinetics with the K_m value of 1.25×10^(-4) M/1 with the V_(max) of 7.14 ㎍/min for casein.
해녀콩 ( Canavalia Lineate ) 잎에서 부분정제한 Ornithine Carbamoyltransferase 에 관하여
Vitamin A 와 Ethanol 이 Dimethylnitrosamine 에 의한 Mouse 조직내의 DNA , RNA 및 단백질의 손상도에 미치는 영향
Anti - Progesterone Monoclonal Antibody 에 대한 Anti - Idiotype 항체의 불임효과에 관한 연구