초파리는 발생과 질병연구를 위한 모델 곤충으로 널리 이용되어 왔다. 본 연구에서도 초파리를 모델곤충으로 한 장질환 연구의 일환으로 다양한 병원균 감염 및 장질환 유발시 어떻게 장줄...
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https://www.riss.kr/link?id=A60153802
2012
English
KCI등재
학술저널
621-626(6쪽)
0
0
상세조회0
다운로드국문 초록 (Abstract)
초파리는 발생과 질병연구를 위한 모델 곤충으로 널리 이용되어 왔다. 본 연구에서도 초파리를 모델곤충으로 한 장질환 연구의 일환으로 다양한 병원균 감염 및 장질환 유발시 어떻게 장줄...
초파리는 발생과 질병연구를 위한 모델 곤충으로 널리 이용되어 왔다. 본 연구에서도 초파리를 모델곤충으로 한 장질환 연구의 일환으로 다양한 병원균 감염 및 장질환 유발시 어떻게 장줄기세포가 작용하는지를 이해하기 위해 초파리 장세포의 일차배양 방법을 확립하였다. 초파리 성충으로부터 장을 해부하고 다양한 효소를 처리하여 장세포를 분리한 후 배양하였다. 배양세포의 생존여부는 현미경 검경 및 MTS assay에 의해 확인한 결과 배양 후 9일째 최대 증식되었고 14일까지 생존함을 확인할 수 있었다. 또한 장줄기세포 및 장내분비세포의 존재도 immunostaining에 의해 확인하였다. 따라서 본 연구에서 구축된 초파리 일차배양 장세포는 다양한 유전자에 의한 장줄기 세포 조절연구뿐만 아니라 장에서 발생하는 다양한 질병을 연구하는 도구로 매우 유용할 것으로 추측된다.
다국어 초록 (Multilingual Abstract)
Drosophila melanogaster has been used as a useful model to study development and disease. In this study, we established the primary culture method of Drosophila in the intestine to understand how intestinal stem cells (ISCs) mediate tissue repair duri...
Drosophila melanogaster has been used as a useful model to study development and disease. In this study, we established the primary culture method of Drosophila in the intestine to understand how intestinal stem cells (ISCs) mediate tissue repair during infection and disease. To obtain intestinal cells, we separated intestines from adult flies and isolated single cells by enzymatic treatment. The survival of cultured cells was measured using MTS-analysis. The maximum growth rate of the cells was observed on the 9th day after seeding. In addition, the presence of ISCs and enteroendocrine cells was confirmed by delta and prospero staining. Accordingly, we supposed that Drosophila melanogaster gut cells established in this study are probably useful in studies about intestinal stem cell regulation and various diseases occurring in the intestine.
목차 (Table of Contents)
참고문헌 (Reference)
1 Amcheslavsky, A., "Tissue damage-induced intestinal stem cell division in Drosophila" 4 : 49-61, 2009
2 Benjamin, O., "The adult Drosophila posterior midgut is maintained by pluripotent stem cells" 439 : 470-474, 2005
3 Vaughn, J. L., "The Establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae)" 13 : 213-217, 1977
4 Palli, S. R., "Studies on apoptosis in a continuous midgut cell line, CF-203, of the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae), In Invertebrate cell culture. Novel directions and biotechnology applications. Science" Enfield 43-51, 1997
5 Wickham, T. J., "Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system" 8 : 391-396, 1992
6 Sicaeros, B., "Primary neuronal cultures from the brains of late stage Drosophila pupae" 4 : 200-, 2007
7 Evans, G. S., "Primary cultures for studies of cell regulation and physiology in intestinal epithelium" 56 : 399-417, 1994
8 Lüer, K., "Primary culture of single ectodermal precursors of Drosophila reveals a dorsoventral prepattern of intrinsic neurogenic and epidermogenic capabilities at the early gastrula stage" 116 : 377-385, 1992
9 Raziel, S. H., "Primary culture of insect midgut cells" 45 : 106-110, 2009
10 Juan, J. G., "Primary and continuous midgut cell cultures from Pseudaletia unipuncta (Lepidoptera: Noctuidae)" 37 : 353-359, 2001
1 Amcheslavsky, A., "Tissue damage-induced intestinal stem cell division in Drosophila" 4 : 49-61, 2009
2 Benjamin, O., "The adult Drosophila posterior midgut is maintained by pluripotent stem cells" 439 : 470-474, 2005
3 Vaughn, J. L., "The Establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae)" 13 : 213-217, 1977
4 Palli, S. R., "Studies on apoptosis in a continuous midgut cell line, CF-203, of the spruce budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae), In Invertebrate cell culture. Novel directions and biotechnology applications. Science" Enfield 43-51, 1997
5 Wickham, T. J., "Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system" 8 : 391-396, 1992
6 Sicaeros, B., "Primary neuronal cultures from the brains of late stage Drosophila pupae" 4 : 200-, 2007
7 Evans, G. S., "Primary cultures for studies of cell regulation and physiology in intestinal epithelium" 56 : 399-417, 1994
8 Lüer, K., "Primary culture of single ectodermal precursors of Drosophila reveals a dorsoventral prepattern of intrinsic neurogenic and epidermogenic capabilities at the early gastrula stage" 116 : 377-385, 1992
9 Raziel, S. H., "Primary culture of insect midgut cells" 45 : 106-110, 2009
10 Juan, J. G., "Primary and continuous midgut cell cultures from Pseudaletia unipuncta (Lepidoptera: Noctuidae)" 37 : 353-359, 2001
11 Wickham, T. J., "Optimization of growth methods and recombinant protein production in BTI Tn-5B1-4 insect cells using the baculovirus expression vector" 9 : 25-30, 1993
12 Wirtz, S., "Mouse models of inflammatory bowel disease" 59 : 1073-1083, 2007
13 Soin, T., "Juvenile hormone analogs do not affect directly the activity of the ecdysteroid receptor complex in insect culture cell lines" 54 : 429-438, 2008
14 Roda, G., "Intestinal epithelial cells in inflammatory bowel diseases" 16 : 4264-4271, 2010
15 Siegmund, B., "Innate and adaptive immunity in inflammatory bowel disease" 17 : 3178-3183, 2011
16 Lanzoni, G., "Inflammatory bowel disease: Moving toward a stem cell-based therapy" 14 : 4616-4626, 2008
17 Ren, F., "Hippo signaling regulates Drosophila intestine stem cell proliferation through multiple pathways" 107 : 21064-21069, 2010
18 Grace, T. D., "Establishment of four strains of cells from insect tissues grown in vitro" 195 : 788-789, 1962
19 Cha, S. J., "Establishment of a novel cell line from Plutella xylostella fat bodies" 29 : 121-126, 1999
20 Apidianakis, Y., "Drosophila melanogaster as a model for human intestinal infection and pathology" 4 : 21-30, 2011
21 Kapuscinski, J., "DAPI: a DNA-specific fluorescent probe" 70 : 220-233, 1995
22 Pan, C., "Comparative proteomic phenotyping of cell lines and primary cells to assess preservation of cell type-specific functions" 8 : 443-450, 2009
23 Davis, T. R., "Baculovirus expression of alkaline phosphatase as a reporter gene for evaluation of production, glycosylation, and secretion" 10 : 1148-1150, 1992
24 O’Reilly, D. R., "Baculovirus Expression Vectors: A Laboratory Manual" Freeman, W. H. and Company 1992
25 Noguchi, T., "A role for actin dynamics in individualization during spermatogenesis in Drosophila melanogaster" 130 : 1805-1816, 2003
26 Granados, R. R., "A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus" 64 : 260-266, 1994
Analysis of Hemolytic Microflora from the Ark Shell (Scapharca broughtonii)
Anti-Obesity Effect of Red Garlic Composites in Rats Fed a High Fat-Cholesterol Diet
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2011-08-03 | 학술지명변경 | 외국어명 : Korean Journal of Life Science -> Journal of Life Science | |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | |
2004-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.37 | 0.37 | 0.42 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.43 | 0.43 | 0.774 | 0.09 |