배양 조건이 Candida albicans의 phospholipase 생성에 미치는 영향 = Effects of Culture Condition on Secretion of Phospholipase from Candida albicans

다국어 초록 (Multilingual Abstract)

Background : The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathoenesis of Candida infections.
Objective : In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans.
Methods : C. albicans ATTCC 10231 was cultivated in various media at 37℃ for 3 days.
Phospholipase activity was measured by fatty acid soap precipitaiton in plate containing 0.04T lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar.
Results : Phospholipase was highly induced when C. albicans was cultivated in broth medium(containing glucose 2%, albumin 0.2% and Fe^++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate.
Conclusion : Highly induction of secreted phospholipase by albumin from C. albicans may be play an importans role in tissue invasion in the pathogenesis of C. albicans.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 1. 실험균주
• 2. 사용배지
• 3. Phospholipase 활성 측정
• 4. 성장곡선
• 실험결과
• 1. 탄소원에 따른 phospholipase의 생성능
• 2. 질소원에 따른 phospholipase 생성능
• 3. 인산염 농도에 따른 phospholipase의 생성능
• 4. 계면 활성제에 의한 영향
• 5. 배양 ph에 의한 영향
• 6. 금속 이온에 의한 영향
• 고찰
• 결론