알코올성 간 손상 조직에서 TGF-β1와 c-Myc, Erb-B2, Thymosin-β4 유전자 발현 융합 연구

국문 초록 (Abstract)

본 연구는 25% 에탄올에 손상된 간조직에서 TGF-β 1와 c-Myc, Erb-B2, Thymosin-β 4 유전자의 발현을 알아 보고 자 실시 하였다. 실험군은 2군으로 나누어 25% 에탄올로 간 손상을 유발한 실험군과 정제수를 투여한 대조군으로 나누어 실험하였다. 검사 결과는 25% 에탄올를 투여 했던 실험군은 대조군에 비하여 TGF-β 1, c-Myc 및 Thymosin-β 4 유전자의 발현 증가를 알 수 있었으며 Erb-B2 유전자는 뚜렷한 발현을 알 수 없었다. 또한 손상된 간 조직에서 헤마톡실린 에오진 염색을 통한 세포 손상을 관찰 할 수 있었다. 결론적으로 기존 임상에서 간 기능 관련 질병 예방과 질환 판정 시 혈청학적, 조직학적 검사 외에 TGF-β 1, c-Myc 및 Thymosin-β 4의 분자 진단 기법에 의한 유전자 발현 상태를 융합 검사함으로써 간 질환 판정의 보조 자료로 활용 될 수 있을 것으로 사료 된다.

다국어 초록 (Multilingual Abstract)

This study has been conducted to see the expression of TGF- β1, c-Myc, Erb-B2 and Thymosin- β4 genes in ethanol - damaged liver tissues. Experimental groups were divided into 2 groups, one where damaged liver was caused by 25% ethanol and normal group administered with purified water. Results of test showed the expression of TGF- β1, c-Myc, and Thymosin- β4 genes was higher in the experimental group treated with 25% ethanol than in the normal group. Erb-B2 gene was not expressed clearly. Thus, it is considered that we can expect to utilize TGF- β1, c-Myc 및 Thymosin- β4 as auxiliary data and find clinical meanings of diagnosis on hepatic diseases, In addition to serologic and histological examination by convergence examining the gene expression status by molecular diagnostic techniques in liver-related disease prevention and diagnosis through results of this study.

목차 (Table of Contents)

• 요약
• Abstract
• 1. 서론
• 2. 재료 및 방법
• 2.1 간 손상 모델
• 2.2 혈청학적 검사
• 2.3 Hematoxylin & Eosin 염색
• 2.4 전자현미경적 조직 관찰
• 2.5 유전자 검사
• 2.6 통계분석
• 3. 결과
• 3.1 전자현미경적 조직관찰
• 3.2 혈청 검사
• 3.3 Hematoxylin & Eosin 염색
• 3.4 유전자 검사
• 4. 고찰 및 결론
• REFERENCES

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